The Use of Cultured Autologous Epidermis in the Treatment of Extensive Burn Wounds

Teepe, R.G.C.; Kreis, R.W.; Koebrugge, Eline J.; Kempenaar, Johanna; Vloemans, A.F.P.M.; Hermans, R.P.; Boxma, H.; Dokter, J.; Hermans, J.; Ponec, Maria; Vermeer, B.J.

doi:10.1097/00005373-199003000-00004

Cited by 113 publications

(29 citation statements)

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“…[53][54][55][56][57] Originally, sandwich and intermingled techniques were used with autografts and human allografts from a skin bank. Nowadays, cultured allografts often are used, as are artificial skin products and other modalities.…”

Section: Discussionmentioning

confidence: 99%

Results of an Internet Survey on the Treatment of Partial Thickness Burns, Full Thickness Burns, and Donor Sites

Hermans¹

2007

Journal of Burn Care & Research

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The objective of this study was to analyze which materials and methods are used for the management of partial and full thickness burns, as well as donor sites. An Internet survey was used to poll directors of burn centers around the world on their preferences for local treatment of different types of burns and donor sites. Results were tabulated and expressed as a percentage of the total number of answers for a given indication. Although many new wound care materials have been launched in the last decade, few of these actually are used widely. The most commonly used materials for partial thickness burns and donor sites are still silver sulphadiazine 1% cream, other antimicrobial ointments and creams and impregnated gauze type dressings. Of the newly available treatment modalities, only two silver dressings were chosen frequently as a primary option for the management of partial thickness burns and donor sites. For full thickness burns, the primary choice is excision and grafting. The diversity of dressings and techniques indicated as preferred in this survey, including many that are known to have side effects, indicates that there is no consensus on topical treatment of partial thickness burns and donor sites. Many respondents prefer "tried and true" materials over newer dressings, particularly if the latter have not been tested in a clinical trial.

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Section: Discussionmentioning

confidence: 99%

Results of an Internet Survey on the Treatment of Partial Thickness Burns, Full Thickness Burns, and Donor Sites

Hermans¹

2007

Journal of Burn Care & Research

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“…We have investigated the possible functional role of this epithelial integrin in normal human keratinocytes, cultured in vitro as originally described by Rheinwald and Green (12,13). Under these conditions (14,15), keratinocyte colonies eventually fuse, giving rise to a tridimensional tissue (stratified squamous epithelium) that can be successfully and permanently transplanted onto patients presenting large skin defects (16)(17)(18)(19)(20)(21) and that maintains characteristics of the original donor site (22,23). Thus, physiological interactions regulating cell adhesion, growth, and differentiation in a complex tissue are potentially accessible for experimental investigations.…”

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confidence: 99%

Polarized integrin mediates human keratinocyte adhesion to basal lamina.

Luca

Tamura²,

Kajiji³

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

198

125

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Epithelial cell interactions with matrices are critical to tissue organization. Indirect immunofluorescence and immunoprecipitations of cell lysates prepared from stratified cultures of human epidermal cells showed that the major integrins expressed by keratinocytes are ad3,4 (also called a644) and a2pl/a3.l1. The adP4 integrin is localized at the surface of basal cells in contact with the basement membrane, whereas a2fil/a3fl1 integrins are absent from the basal surface and are localized only on the lateral surface of basal and spinous keratinocytes. Anti-fl4 antibodies potently inhibited keratinocyte adhesion to matrigel or purified laiinin, whereas anti-fl1 antibodies were ineffective. Only anti-fl4 antibodies were able to detach established keratinocyte colonies. These data suggest that aEP4 mediates keratinocyte adhesion to basal lamina, whereas the ,BI subfamily is involved in cell-cell adhesion of keratinocytes.

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“…Secondary infection by some bacteria, however, is a possible problem when implanting such cultured skin substitutes. Therefore, protecting wound skin using skin substitutes is the primary treatment, followed by drug therapies with antibacterial ointments (7,8). A greater therapeutic effect may be obtained by direct application of a cultured skin substitute containing antibiotics as a drug formulation on wound skin.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Evaluation of Gene-transfected Cultured Skin as a Novel Drug Delivery System for Severely Burned Skin

et al. 2007

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The Use of Cultured Autologous Epidermis in the Treatment of Extensive Burn Wounds

Cited by 113 publications

References 0 publications

Results of an Internet Survey on the Treatment of Partial Thickness Burns, Full Thickness Burns, and Donor Sites

Results of an Internet Survey on the Treatment of Partial Thickness Burns, Full Thickness Burns, and Donor Sites

Polarized integrin mediates human keratinocyte adhesion to basal lamina.

Preparation and Evaluation of Gene-transfected Cultured Skin as a Novel Drug Delivery System for Severely Burned Skin

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