The use of dentin matrix scaffold and dental follicle cells for dentin regeneration

Guo, Weihua; He, Yueqiu; Zhang, Xiaojun; Lu, Wei; Wang, Chunmei; Yu, Hua; Liu, Yuan; Li, Yuan; Zhou, Yuefei; Zhou, Jing; Zhang, Manjing; Deng, Zhihong; Jin, Yan

doi:10.1016/j.biomaterials.2009.08.034

Cited by 144 publications

(141 citation statements)

References 53 publications

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“…If this environment is created, locally derived progenitor/stem cells adjacent to an injury can be recruited for pulp regeneration (Kim et al, 2010a(Kim et al, , 2010b. The delivery of therapeutic agents can be conducted via both protein-and gene-based approaches (Nakashima and Reddi, 2003;Nakashima, 2005;Nakashima and Akamine, 2005; Batouli et al, 2003;Gronthos et al, 2000Gronthos et al, , 2002Prescott et al, 2008;Miura et al, 2003;Cordeiro et al, 2008SHED Sonoyama et al, 2006Huang et al, 2008;Sonoyama et al, 2008SCAP Morsczeck et al, 2005Wu et al, 2008;Guo et al, 2009;Tsuchiya et al, 2010DFPCs Iohara et al, 2009SPCs Hu et al, 2006Sloan and Smith, 2007;Morsczeck et al, 2008BMSCs Biofactors BMPs Iohara et al, 2004Nakashima and Reddi, 2003;Nakashima, 2005TGFβ Chan et al, 2005Liu et al, 2007FGF-2 Ishimatsu et al, 2009DSP Suzuki et al, 2009DPP Suzuki et al, 2009BSP Decup et al, 2000DMP-1 Srinivasan et al, 1999Smith et al, 1995;He et al, 2003;Narayanan et al, 2003;He and George, 2004;Almushayt et al, 2006;Prescott et al, 2008...…”

Section: Pulp Tissue-engineering Triadmentioning

confidence: 99%

“…Dentine chips Cordeiro et al, 2008;Guo et al, 2009MTA Bogen et al, 2008Kuratate et al, 2008 Fibrin Bashutski andWang, 2008;Danilović et al, 2008Collagen Prescott et al, 2008Iohara et al, 2009;Kim et al, 2010aGelatin Ishimatsu et al, 2009PGA Mooney et al, 1996Bohl et al, 1998;Buurma et al, 1999PLG Huang et al, 2010PLGA El-Backly et al, 2008Composites Shi et al, 2005Mao et al, 2010 BMSCs, bone marrow mesenchymal stem cells; BSP, bone sialoprotein; DFPCs, dental follicle progenitor cells; DMP-1, dentine matrix protein-1; DPP, dentine phosphoprotein; DPSCs, dental pulp stem cells; DSP, dentine sialoprotein; FGF-2, fibroblast growth factor-2; MEPE, matrix extracellular phosphoglycoprotein; MTA, mineral trioxide aggregate; PDLSCs, periodontal ligament stem cells; PGA, polyglycolic acid; PLG, poly-D,L-lactide and glycolide; PLGA, poly(lactic-co-glycolic) acid; SCAP, stem cells from apical papilla; SHED, stem cells from exfoliated deciduous teeth; SPCs, side-population cells (from the pulp); TGFβ, transforming growth factor-β.…”

Section: Pulp Tissue-engineering Triadmentioning

confidence: 99%

“…Although the origin, identification and localization of these dental pulp stem remain under consideration, optimiztic research on stem cells permits us to believe that knowledge about DPSCs will lead to their use in clinical therapeutics for potential tooth and pulp regeneration (Yen and Sharpe, 2008). Besides the two principal properties (self-renewal and multilineage differentiation) of stem cells, DPSCs have some other advantages associated with their application in tissue-engineering: (a) access to the collection site of these cells is easy and produces very low morbidity; (b) extraction of stem cells from pulp tissue is highly efficient; (c) they have an extensive differentiation e6 H.-H. Sun et al Dentine and periodontal regeneration Morsczeck et al, 2005;Wu et al, 2008;Guo et al, 2009;Tsuchiya et al, 2010 DFPCs, dental follicle progenitor cells; DPSCs, dental pulp stem cells; SCAP, stem cells from apical papilla; SHED, stem cells from exfoliated deciduous teeth; PDLSCs, periodontal ligament stem cells.…”

Section: Stem Cells Of Dental Originmentioning

confidence: 99%

“…In general, this strategy involves harvesting tissue biopsies, expanding cell populations and subsequent transplantation back into the disinfected pulp space, normally via information-enriched biomaterials, with an attempt to regenerate lost pulp tissues (components that may be involved in this process are shown in Table 1) meaningful insights into the development of dental tissues and cellular differentiation processes (Morsczeck et al, 2008). To date, as reviewed by Huang recently, five types of human dental stem cells have been isolated and characterized (Huang, 2009b): (a) DPSCs (Gronthos et al, 2000(Gronthos et al, , 2002Batouli et al, 2003); (b) SHED ; (c) SCAP (Sonoyama et al, 2006;Huang et al, 2008;Sonoyama et al, 2008); (d) periodontal ligament stem cells (PDLSCs) (Seo et al, 2004;Shi et al, 2005;Sonoyama et al, 2006); and (e) dental follicle progenitor cells (DFPCs) (Morsczeck et al, 2005;Wu et al, 2008;Guo et al, 2009;Tsuchiya et al, 2010). All of these stem cells demonstrated multipotentiality and the capability to regenerate multiple dental/periodontal tissues in in vitro and in vivo investigations (Huang, 2009b) (Table 2).…”

Section: Stem Cells Of Dental Originmentioning

confidence: 99%

“…The knowledge gained from adult stem cell biology, especially associated with dentine, bone and periodontal tissue regeneration, provides additional opportunities for eventual tooth organogenesis (Butler and Ritchie, 1995). Finally, the success of regenerative endodontic therapy is dependent upon close cooperation between medical doctors and biomaterials scientists to create new clinical treatment modalities that will allow clinicians to yield a functional pulp tissue within cleaned and shaped root canal systems to revitalize teeth (Murray and García-Godoy, 2006;Guo et al, 2009). In the future, pulp tissue may be regenerated by an in vivo approach, which involves the delivery into root canals of a combination of autologous or allogenic stem cells and appropriate scaffolds incorporated with specific growth factors; or pulp implantation (an ex vivo approach) involving the surgical implantation of engineered pulp tissue grown in the laboratory and relying on the tissue-engineering triad.…”

Section: Closing Remarks and Future Perspectivesmentioning

confidence: 99%

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Biological approaches toward dental pulp regeneration by tissue engineering

Sun

Jin

et al. 2010

J Tissue Eng Regen Med

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Root canal therapy has been the predominant approach in endodontic treatment, wherein the entire pulp is cleaned out and replaced with a gutta-percha filling. However, living pulp is critical for the maintenance of tooth homeostasis and essential for tooth longevity. An ideal form of therapy, therefore, might consist of regenerative approaches in which diseased/necrotic pulp tissues are removed and replaced with regenerated pulp tissues to revitalize the teeth. Dental pulp regeneration presents one of the most challenging issues in regenerative dentistry due to the poor intrinsic ability of pulp tissues for self-healing and regrowth. With the advent of modern tissue engineering and the discovery of dental stem cells, biological therapies have paved the way to utilize stem cells, delivered or internally recruited, to generate dental pulp tissues, where growth factors and a series of dentine extracellular matrix molecules are key mediators that regulate the complex cascade of regeneration events to be faithfully fulfilled.

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Section: Pulp Tissue-engineering Triadmentioning

confidence: 99%

Section: Pulp Tissue-engineering Triadmentioning

confidence: 99%

Section: Stem Cells Of Dental Originmentioning

confidence: 99%

Section: Stem Cells Of Dental Originmentioning

confidence: 99%

Section: Closing Remarks and Future Perspectivesmentioning

confidence: 99%

See 3 more Smart Citations

Biological approaches toward dental pulp regeneration by tissue engineering

Sun

Jin

et al. 2010

J Tissue Eng Regen Med

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Stem and Progenitor Cells of Dental and Gingival Tissue Origin

Morsczeck¹,

Huang²,

Shi³

2013

Stem Cells in Craniofacial Development and Regeneration

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Nanofibrous Spongy Microspheres Enhance Odontogenic Differentiation of Human Dental Pulp Stem Cells

Kuang

Zhang

Jin

et al. 2015

Adv Healthcare Materials

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While dental caries afflicts nearly 100% adults and 60–70% children, dentin regeneration is challenging due to its complicated anatomical structure and the shortage of odontoblasts. In this study, a novel injectable cell carrier, nanofibrous spongy microspheres (NF-SMS), was developed for dentin regeneration. Biodegradable and biocompatible poly(L-lactic acid)-block-poly(L-lysine) copolymers were synthesized and fabricated into NF-SMS using self-assembly and thermally-induced phase separation techniques. We hypothesized that NF-SMS with interconnected pores throughout the nanofibrous microspheres could enhance the proliferation and odontogenic differentiation of human dental pulp stem cells (hDPSCs), compared to nanofibrous microspheres (NF-MS) without pore structure and conventional solid microspheres (S-MS) with neither nanofibers nor pore structure. During the first 9 d in culture, hDPSCs proliferated significantly faster on NF-SMS than on NF-MS and S-MS (p < 0.05). Following in vitro odontogenic induction, all the examined odontogenic markers including ALP content, OCN, BSP, COL1, DSPP gene expression levels, calcium content and DSPP protein content were found significantly higher in the NF-SMS group than in the NF-MS and S-MS control groups. Furthermore, 6 wk after subcutaneous injection of hDPSCs and microspheres into nude mice, histological analysis showed that NF-SMS supported superior dentin-like tissue formation compared to NF-MS and S-MS. Taken together, the results supported our hypothesis and suggest that the novel NF-SMS have great potential as an injectable cell carrier for dentin regeneration.

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The use of dentin matrix scaffold and dental follicle cells for dentin regeneration

Cited by 144 publications

References 53 publications

Biological approaches toward dental pulp regeneration by tissue engineering

Biological approaches toward dental pulp regeneration by tissue engineering

Stem and Progenitor Cells of Dental and Gingival Tissue Origin

Nanofibrous Spongy Microspheres Enhance Odontogenic Differentiation of Human Dental Pulp Stem Cells

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