The use of derivatized magnetoliposomes for extraction of antibodies from aqueous solutions

Dumitrascu, Gabriela; Kumbhar, Amar; Zhou, Weilie; Rosenzweig, Zeev

doi:10.1109/20.951351

Cited by 11 publications

(6 citation statements)

References 7 publications

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“…However, conversely to typical Stern−Volmer quenching behavior, which is driven by collisions between quencher and luminescent molecules, the quenching of the luminescence of the QDs is attributed to ion binding followed by a redox reaction on the surface of the QDs. This results in a nonreversible luminescence quenching similar to the one observed when antibodies against fluorescence dyes such as fluorescein, rhodamine, and Texas Red bind to their corresponding antigens . Since the response of the CdS QDs to copper ions is not reversible, they cannot be used for real-time monitoring of large ion waves.…”

Section: Resultsmentioning

confidence: 99%

“…This results in a nonreversible luminescence quenching similar to the one observed when antibodies against fluorescence dyes such as fluorescein, rhodamine, and Texas Red bind to their corresponding antigens. 36 Since the response of the CdS QDs to copper ions is not reversible, they cannot be used for real-time monitoring of large ion waves. Nevertheless the large dynamic range enables multiple determinations of copper ion concentrations in diluted copper ion solutions.…”

Section: Resultsmentioning

confidence: 99%

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Luminescent CdS Quantum Dots as Selective Ion Probes

2002

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Water-soluble luminescent CdS quantum dots (QDs) capped by polyphosphate, L-cysteine, and thioglycerol were synthesized in aqueous solution. The ligands were found to have a profound effect on the luminesence response of CdS QDs to physiologically important metal cations. Polyphosphate-capped CdS QDs were sensitive to nearly all mono- and divalent cations, showing no ion selectivity. Conversely, thioglycerol-capped CdS QDs were sensitive to only copper and iron ions. Similar concentrations of physiologically relevant cations, such as zinc, sodium, potassium, calcium, and magnesium ions did not affect the luminescence of thioglycerol-capped CdS QDs. On the other hand, L-cysteine-capped CdS QDs were sensitive to zinc ions and insensitive to other physiologically important cations, such as copper, calcium, and magnium ions. To demonstrate the detection capability of these new ion probes, L-cysteine and thioglycerol-capped CdS QDs were used to detect zinc and copper ions in physiological buffer samples. The detection limits were 0.8 microM for zinc (II) and 0.1 microM for copper (II) ions. The emission enhancement of the QDs by zinc (II) is attributed to activation of surface states, whereas the effective reduction of copper (II) to copper (I) may explain the emission decrease of the thioglycerol-capped CdS QDs when charged with copper ions. Unlike organic fluorescent dyes, the thioglycerol-capped luminescent CdS QDs discriminate between copper and zinc ions and are therefore suitable for the analysis of copper ions in biological samples in the presence of physiological concentrations of zinc ions. The interference of iron ions with zinc and copper ion detection is attributed to an inner filter effect, which is eliminated by adding fluoride ions to form the colorless complex FeF6(3-). To the best of our knowledge, this is first use of luminescent semiconductor quantum dots as selective ion probes in aqueous samples.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Luminescent CdS Quantum Dots as Selective Ion Probes

2002

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“…This is a major advantage over conventional magnetic separation techniques. For example, the detection and extraction of antibodies from aqueous solutions, using fluorescent magnetoliposomes, has been reported by Rosenzweig and co-workers [ 72 ]. The fluorescent magnetoliposomes consist of cobalt platinum alloy nanoparticles encapsulated in liposomes, which have been labelled with BODIPY-Fluorescein dye.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Multifunctional Magnetic-fluorescent Nanocomposites for Biomedical Applications

2008

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Nanotechnology is a fast-growing area, involving the fabrication and use of nano-sized materials and devices. Various nanocomposite materials play a number of important roles in modern science and technology. Magnetic and fluorescent inorganic nanoparticles are of particular importance due to their broad range of potential applications. It is expected that the combination of magnetic and fluorescent properties in one nanocomposite would enable the engineering of unique multifunctional nanoscale devices, which could be manipulated using external magnetic fields. The aim of this review is to present an overview of bimodal “two-in-one” magnetic-fluorescent nanocomposite materials which combine both magnetic and fluorescent properties in one entity, in particular those with potential applications in biotechnology and nanomedicine. There is a great necessity for the development of these multifunctional nanocomposites, but there are some difficulties and challenges to overcome in their fabrication such as quenching of the fluorescent entity by the magnetic core. Fluorescent-magnetic nanocomposites include a variety of materials including silica-based, dye-functionalised magnetic nanoparticles and quantum dots-magnetic nanoparticle composites. The classification and main synthesis strategies, along with approaches for the fabrication of fluorescent-magnetic nanocomposites, are considered. The current and potential biomedical uses, including biological imaging, cell tracking, magnetic bioseparation, nanomedicine and bio- and chemo-sensoring, of magnetic-fluorescent nanocomposites are also discussed.

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“…Recently, gold-based bimetallic nanoparticles are emerging as potential catalysts for fuel-cell reactions. − An example is the use of gold−platinum alloys as electrocatalysts for methanol oxidation 29 and oxygen reduction 30 reactions. The use of bimetallic nanoparticles for bioanalytical and biomedical probes has also been reported. In these examples, an understanding of the nanoparticle assembly and the interparticle interactions is very important.…”

Section: Introductionmentioning

confidence: 99%

Assembly of Bimetallic Gold−Silver Nanoparticles via Selective Interparticle Dicarboxylate−Silver Linkages

et al. 2005

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This article describes findings of an investigation of the thin-film assembly of binary metal nanoparticles via interparticle linkages of dicarboxylic acid mediators at selective metal sites. Decanethiol-(DT-) capped gold-silver (Au-Ag) alloy nanoparticles of ∼3-nm core sizes were chosen as a model system because of the unique carboxylate-Ag + binding chemistry and the well-defined thiolate-capping capability at Au. The dicarboxylate-mediated thin-film assembly of Au-Ag nanoparticles was characterized using UV-vis, TEM, XPS, FTIR, and NMR techniques. The results from controlling the oxidative or reductive conditions reveal that the oxidation of Ag to Ag + on the alloy nanocrystal surface played an important role in the selective ligand exchange and interparticle linkage by dicarboxylic acids (e.g., hexadecanedioic acid, HDA). The formation of the thin-film assembly is dependent on both the alloy composition of the Au-Ag nanoparticles and the chain length of the dicarboxylic acids. The degree for an HDA-DT/AuAg exchanging and linking system is determined to yield a value of 16% of DTs per Au-Ag particle being displaced by HDAs. In comparison to similar thin-film assemblies of Au-Ag and Au nanoparticles by other mediators, the results support the conclusion that the HDA-mediated assembly of Au-Ag nanoparticles occurs via -CO 2 --Ag + linkages at silver sites forming a mediator (HDA)-template (DT) nanostructure. The nanostructured thin-film assemblies exhibit interesting optical and electronic properties, which have important implications for the exploration of binary nanoparticle-structured materials for sensing and catalytic applications where fine-tuning of either the binary nanocrystal cores or the bifunctional interparticle structures is desired.

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The use of derivatized magnetoliposomes for extraction of antibodies from aqueous solutions

Cited by 11 publications

References 7 publications

Luminescent CdS Quantum Dots as Selective Ion Probes

Luminescent CdS Quantum Dots as Selective Ion Probes

Multifunctional Magnetic-fluorescent Nanocomposites for Biomedical Applications

Assembly of Bimetallic Gold−Silver Nanoparticles via Selective Interparticle Dicarboxylate−Silver Linkages

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