The use of DNA SCAR markers for differentiation of Kamchatka mykiss (Parasalmo (Oncorhynchus) mykiss)

Introduction of ISSR markers for Kamchatka mykiss (Parasalmo (Oncorhynchus) mykiss) (Walbaum) (Salmonidae, Salmoniformes)

“…Thus, the results of this study are consistent with the data obtained in our previous work with the use of allozymes [2] as well as SCAR and microsatellite markers [3,4] and demonstrates the applicability of ISSR markers for further differentiation of the Kam chatka mykiss populations. Cost efficiency, good reso lution, and simplicity of this method make it possible to perform screening of a large number of populations in a fairly short time and with a minimum of equip ment.…”

“…The phyloge netic subdivision of the Kamchatka mykiss popula tions was studied insufficiently and primarily by our group of authors. In the previous studies, the geo graphical differentiation was determined using alloz ymes [2], SCAR markers developed in our laboratory [3], and microsatellite locus analysis [4]. The aim of the present study was to develop new techniques and increase the number of such population significant genetic mark ers by analyzing poorly studied DNA regions.…”

“…Earlier, we have created seven original ISSR markers specifically for this fish species [7]. In the present study, we evalu ated the efficiency of using these markers for genetic differentiation of myukiss populations in Kamchatka.The study was performed on the same material ( Table 1) that was used in the previous study per formed with SCAR markers [3].The DNA isolation method, the tests for determi nation of DNA quantity and quality, the selection of ISSR primers, and PCR conditions were described in detail in our methodical work on the development of ISSR markers [7]. The reproducibility of results in dif ferent experiments was tested three times using seven pairs of primers.…”

“…The study was performed on the same material ( Table 1) that was used in the previous study per formed with SCAR markers [3].…”