The use of electrochemical impedance spectroscopy for biosensing

Lisdat, Fred; Schäfer, Daniel

doi:10.1007/s00216-008-1970-7

Cited by 763 publications

(480 citation statements)

References 170 publications

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“…The experimental data of the peak current as a function of scan rates (not shown) were further studied to calculate the surface coverage    of the deposited polymeric film in (mol cm 2 ) applying Eq. (1) [34].…”

Section: Electrochemical Polymerization Of 4-adpa Using CV Methodsmentioning

confidence: 99%

Fabrication and surface characterization of poly(4-aminodiphenylamine) film modified electrode and its application for lead and cadmium determination

Al-Hinaai

Al-Harthi

Khudaish

2014

SQU Journal for Science

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Poly(4-aminodiphenylamine), Padpa, film was synthesized electrochemically on a glassy carbon electrode by potentiodynamic repetitive cycling of 4-aminodiphenylamine (4-adpa) in 1.0 M HCl. The mechanistic steps involved the oxidation of the protonated monomer to diimine species followed by its dimerization to form the mono-charged radical intermediate, which was considered as the initiation step for the progress of polymerization. The electrochemical properties and surface morphology of the film modified electrode were characterized using electrochemical and various surface scanning techniques. The XPS data demonstrated the existence of =N bonding responsible for polymer formation, while the AFM image revealed a uniform and symmetrical fiber structure with low energy dissipation. The modified electrode was primarily applied as an environmental sensor for the simultaneous and selective determination of Cd 2+

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Section: Electrochemical Polymerization Of 4-adpa Using CV Methodsmentioning

confidence: 99%

Fabrication and surface characterization of poly(4-aminodiphenylamine) film modified electrode and its application for lead and cadmium determination

Al-Hinaai

Al-Harthi

Khudaish

2014

SQU Journal for Science

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“…Electrical detection methods are appealing because of their low cost, low power consumption, ease of miniaturization, and potential multiplexing capability [38,39]. The basis of these detection systems stems from a binding-induced change in some electrical property of the circuit of which the sensor is a vital component.…”

Section: Electrochemicalmentioning

confidence: 99%

“…Electrochemical impedance spectroscopy (EIS) is a method for probing the features of an electrode surface modified by such immunocomplexes. In EIS, sensing is accomplished by measuring changes in the resistance and/or capacitance of the electrode-solution interface upon binding of a target molecule to a receptor functionalized surface [38,39]. EIS does not require the use of enzyme labels to report binding events which contrasts with other electrical measurements, such as amperometry, potentiometry and voltammetry.…”

Section: Amperometric Sensorsmentioning

confidence: 99%

Trends and Perspectives in Immunosensors

2012

Antibodies Applications and New Developments

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Immunosensors are devices that comprise both a biomolecular recognition system, such as an antibody and its corresponding antigen, and a transducer to translate the high affinity and specific binding event into a physical signal.Antibodies are produced by an immunological response to the presence of a foreign substance called an antigen. Antibodies may be immobilised onto a variety of platforms including bulk planar surfaces and nanoparticles by either covalent or adsorption strategies. Different interfaces between the bio-components and the detector are available to monitor in 'real-time' the signal generated by biological interactions. The transducers detect, for example, the change in electron transfer, absorbance, fluorescence, refractive index, mass change or heat transfer as the antibody binds to the antigen of interest. Such analytical devices have allowed a wide range of analytes to be identified and quantified such as pathogens, toxins, environmental food contaminants and disease biomarkers.The demand for sensitive, rapid, 'on-site' techniques has taken advantage of the latest advances in microfluidics and nanotechnology. This chapter will highlight current trends in immobilisation, micro/nano-fluidics/ and transducers utilised. A number of examples outlining the exploitation of these elements in immunosensors and their successful applications will be described.

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“…Electrochemical impedance sensors are fast emerging as rapid, reliable and cost effective. EIS is a simple but powerful technique for monitoring the changes on the surface of electrodes [8][9][10][11]. This technique is advantageous because it can be used to monitor events happening over a broad range of times.…”

Section: B Ide As Impedimetric Sensormentioning

confidence: 99%

Evaluation of Nanomaterials-biomolecule hybrids for signal enhancement of impedimetric biosensors

Singh

Dash

Whited

et al. 2011

2011 11th IEEE International Conference on Nanotechnology

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-Five different Nano-materials (NMs) were used for making hybrid conjugates with Protein-A (PrA), a biomolecule that binds specifically with antibodies (Abs). The choice of NMs (Gold, CdS, SrTiO 3 , Graphene Oxide and Polystyrene) exhibit both inorganic and organic NMs, with a wide range of electrical characteristics i.e., from metal, semiconductor to non conducting dielectric materials. The NMs-PrA conjugates prepared were characterized using UVvisible spectroscopy and fluorescence microscopy before use for biosensor application. These hybrids were than compared for their relative efficacy in enhancing the biosensor signal as recorded using faradaic electrochemical impedance spectroscopy (EIS). A nanogap interdigitated electrodes array (IDEA) sensor was coated with rabbit IgG antibodies and the binding signal from NM-PrA was compared with that of PrA binding alone. The EIS signal was analyzed by fitting the data to a Randles equivalent circuit. Values of two prime circuit elements i.e., charge transfer resistance (R-Ct) and double layer capacitance (C-dl) were determined from the best fit. The difference between the two signals provided the insight into the mechanisms for different NMs and the relative efficiency in signal enhancement.

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The use of electrochemical impedance spectroscopy for biosensing

Cited by 763 publications

References 170 publications

Fabrication and surface characterization of poly(4-aminodiphenylamine) film modified electrode and its application for lead and cadmium determination

Fabrication and surface characterization of poly(4-aminodiphenylamine) film modified electrode and its application for lead and cadmium determination

Trends and Perspectives in Immunosensors

Evaluation of Nanomaterials-biomolecule hybrids for signal enhancement of impedimetric biosensors

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