The Use of Electrophoretic Isozymes to Detect Tungro Infected Rice

Suranto, Suranto; Arief, Andi Adri; Supyani,

doi:10.17503/agrivita.v39i2.643

Cited by 6 publications

(6 citation statements)

References 8 publications

(10 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This means that using this enzyme activity, ten local sweet potatoes can be distinguished. Recent studies dealing with peroxidase isozyme had been conducted by Suranto et al (2017), who noted that not only the total number of isozyme bands was useful in distinguishing the difference between the healthy and the virus-infected plants, but this enzyme activity on both samples was also able to compare, especially through their band thickness, between the healthy and the diseased plants. Other interesting results of the above two isozymes were reported by Hidayati et al (2018) dealing with Cucurbita moschata at different altitudes.…”

Section: Peroxidase Isozymementioning

confidence: 99%

“…This occurrence may have been correlation with defense mechanism of the plant, particularly when they grow under unfavorable environmental condition. This phenomenon was partly explained by Suranto et al (2017) in detecting rice infected virus. These studies were conducted to have a look at whether variation of morphological character of ten local sweet potatoes would be shown also on their appearance of pollen morphology and also the isozyme banding patterns of peroxidase.…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia

2019

Self Cite

View full text Add to dashboard Cite

Abstract. Suranto, Wahyuni D, Edy Purwanto E. 2019. Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia. Biodiversitas 20: 2511-2518. Sweet potato (Ipomoea batatas Lam) has been recorded to have many cultivars and variations in the morphological characters not only for the leaves but also for the tubers. Sweet potatoes had economically significant role, especially as an additional food due to its high content of carbohydrate and other nutrient values. In order to test whether the morphological variation in this species was also expressed at the micro character; such as pollen morphology and enzyme activity, ten sweet potato cultivars were used in this study. The morphological characters such as shape and structure of the pollen features were examined using Scanning Electron Microscopy (SEM). Accordingly, Polyacrylamide Gel Electrophoresis (PAGE) was used to look-at the banding pattern of peroxidase isozymes. Morphological characters of leaves, flowers, and tuber were observed qualitatively both for the typical shape and colors. More detail of flower shapes was drown manually especially their petal edges. The results showed that there was variation among the ten sweet potato cultivars confirmed not only on their morphological characters but also the isozyme banding patterns of ten cultivars tested. At least, two cultivar Antin 3 and Papua Solossa had uniques characters especially the purple stem and yellow tuber and dark skin on the tuber, respectively. In addition, the structural analysis of pollen showed that Beta 1 cultivar has very large pollen; 116,50 μm (axis polar) and the contour surface of exine pollen was concave with the aperture shape and ornamentation were prolate spheroidal and echinate respectively. This unique character was also shown by its peroxidase isozyme bands. Eleven bands of leaf and 13 bands of stem samples were detected respectively. The typical Rf values of 0.31, 0.48 and 0.41 were detected for the leaf, while for stem samples were 0.14, 0.20 and 0.26. The Beta 1 cultivar showed very thick bands in between Rf values of 0.13 and 0.26. Meanwhile, the unique band pattern of Papua Salosa was the absence of band at 0.38.

show abstract

Section: Peroxidase Isozymementioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia

2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Penelusuran virulensi serangga khususnya pada N. virescens dapat dikategorikan sebagai pendekatan deteksi dini berdasarkan vektor. Walaupun sudah ada deteksi penyakit tungro berdasarkan DNA virus pada tanaman (Suranto et al 2017;Ladja et al 2018), ekspresi kandidat protein masih perlu diidentifikasi identitasnya sebagai protein tertentu dan dipelajari interaksi proteinprotein menggunakan Mass-spectrometry (MS) (Szabo and Janaky 2015).…”

Section: Kemampuan Penularan Tungrounclassified

Hubungan Antara Profil Protein Populasi Vektor Wereng Hijau Nephotettix virescens dan Gejala Penularan Tungro pada Tanaman Padi

Senoaji

Rahardjo

Tarno

2021

j. penelit. pertan. tanam. pangan

View full text Add to dashboard Cite

Nephotettix virescens is the most effective vector for transmitting tungro disease to rice plants. Two different viral particles cause Tungro transmission. Disease control was often not anticipated in the field, especially when planting is asynchronous, that cause been detected lately. At the cellular level, vector interactions with viruses indicate vector proteins response to viral in the body of vector insects which involved in virus transmission in plants. This study aims to describe the relationship between the differentiation of N. virescens vector protein profiles on the types of tungro symptoms resulting from the transmission to develop techniques for early detection and control of the transmission process. The workflow of this study is screening on vector insects to obtain protein candidates thought to have a role in tungro transmission that had never been previously reported. The results of this study suggested that proteins with estimated molecular weights of 132, 73, and 49 kDa are candidates for proteins that can be used for screening purposes or virulent vector tracing as an early warning alternative to control tungro disease in endemic areas.

show abstract

“…Beside fertilizer selection, a crop farming system needs an early detection system to prevent harvesting failure [45]. Thus, the promising approach of sustainable agriculture has to do some innovations, not only for natural resource usage but also for micro and macrofaunal supporting plant growth or as a natural predator of pests and diseases.…”

Section: The Challenges and Opportunities Of Indigenous Sources Development As Organic Fertilizer Materialsmentioning

confidence: 99%

Potential Availability of Indigenous Resources to Reduce Agriculture Environmental Problems in Klaten, Indonesia

Soebandiono¹,

Muhibuddin²,

Purwanto³

et al. 2021

Int. J. Adv. Sci. Eng. Inf. Technol.

View full text Add to dashboard Cite

Inorganic fertilizer usage in high doses for rice cultivation in Indonesia has become a sustainable agriculture threat. Klaten has approximately more than 50% of the total areas were used for agricultural land. It needs a huge amount of agricultural input, especially fertilizer requirements. It is better to select a fertilizer that concerns soil conservation and is more environmentally friendly, i.e. organic fertilizer implementation, utilizing the materials to ensure its adequate availability. This research aims to identify the potential availability of organic fertilizer materials, which the farmers usually use in Klaten, and analyze how farmers' existing conditions can utilize the indigenous sources as organic fertilizer materials. This research used a descriptive method by using the data from field observation. The results showed that the potential availability of organic fertilizers in Klaten, such as Local Microorganism (MOL) of the banana hump, papaya fruit, and pineapple fruit, are 24,493.55 liters per year. They can cover about 979,742 ha paddy field area; Plant Growth Promoting Rhizobacteria (PGPR) of the bamboo root is 210,290 liters per year, and it can cover about 8,412 ha paddy field area; and animal manure composted of cows, goats, sheep, buffaloes, horses, and chickens' dung is 101,841.91 tons per year, and it can cover about 20,368 ha paddy field area. The majority of the farmers used indigenous organic fertilizer because of their benefit for low-cost production and the ease of getting it.

show abstract

The Use of Electrophoretic Isozymes to Detect Tungro Infected Rice

Cited by 6 publications

References 8 publications

Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia

Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia

Hubungan Antara Profil Protein Populasi Vektor Wereng Hijau Nephotettix virescens dan Gejala Penularan Tungro pada Tanaman Padi

Potential Availability of Indigenous Resources to Reduce Agriculture Environmental Problems in Klaten, Indonesia

Contact Info

Product

Resources

About