The Use of Enrichment Serology for Salmonella Detection in Human Foods and Animal Feeds

Abstract

Samples (2208) of food raw materials and products were examined for the presence of salmonellae by use of conventional salmonella, detection procedures and the enrichment serology (ES) techniques described by Sperber & Deibel (1980); 348 samples were positive for salmonellae by the conventional procedures. Using the ES technique with a 24 h elective enrichment step, 93-98% of samples positive by the conventional procedures were also positive by the ES technique. Selective enrichment of food samples using tetrathionate broth containing novohiocin, incubated at 41 ', led to the best recovery of salmonellae by both the conventional and ES techniques. ENRIUHMENT S E R o L o w , an accelerated procedure for detecting salmonellae in foods, was described by Sperber t Deibel in 1969. This involved pre-enrichment and * Enrichment serology with 24 h elective enrichment. b Selective enrichment followed by plating on brilliant green agar after 24 h or 48 h. 0 Not tested.

Improved Salmonella recovery from moderate to highly polluted waters

“…The findings are in agreement with previous results reported for sodium novobiocin addition to the enrichment broth (Jeffries 1959) or to the plating media (Hargrove et al 1971 ;Boothroyd & Baird-Parker 1973;Hoben et al 1973;Restaino et al 1977). The method described in the We especially thank Manuel Serra for his generous contributions to the statistical analysis.…”

“…Several authors (Jeffries 1959;Hargrove et al 1971;Boothroyd & Baird-Parker 1973;Hoben et al 1973;Restaino et al 1977) This antibiotic inhibits the growth of Proteus, Pseudomonas and Escherichia coli which are usually present in large numbers in surface waters. A much modified Rappaport medium (R10/43"C) recently described (Vassiliadis et al 1978) permits the recovery of a wide range of salmonellas.…”

Influence of Resident Salmonella on Contamination of Broiler Flocks

“…Salmonella were isolated by enrichment serology (Sperber and Deibel, 1969;Boothroyd and Baird-Parker, 1973) as adapted for poultry products by Lahellec and Colin (1977) and by conventional method. The conventional method entailed preenrichment at 37 C for 18 h in buffered peptone solution followed by enrichment in tetrathionate broth (no.…”

A MINIATURIZED ENRICHMENT SEROLOGY METHOD FOR RAPID DETECTION of SALMONELLA FROM POULTRY MEAT and REARING FARMS ENVIRONMENT

“…In the present study, we applied the same concept of miniaturization to the enrichment serology technique used for Salmonella detection introduced by Sperber and Deibel (1969) and adapted by Boothroyd and Baird-Parker (1973). This agglutination technique uses polyclonal flagellar antisera.…”