The Use of Fibrin Glue in Skin Grafts and Tissue-Engineered Skin Replacements: A Review

Currie, Lachlan; Sharpe, Justin R.; Martin, Robert H.

doi:10.1097/00006534-200111000-00045

Cited by 367 publications

(257 citation statements)

References 90 publications

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“…The use of fibrin glue as a carrier of diverse elements is not new in literature however the discovery that it would be able to keep the NGF active longer, gradually liberating it in the nerve repair was considered a great advance in microsurgery 6 .…”

Section: Many Authors Have Recently Enhanced the Results Of End-mentioning

confidence: 99%

“…However Zeng et al 3 described a liberation peak in 18 hours which is progressively reduced until its complete extinction in 14 days. Differently Currie et al 6 referred that the fibrin glue is no longer found in the repair site 10 days after its application, limiting the NGF action for a longer period of time.…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, the role of fibrin glue as a carrier and stabilizer of NGF is recent in literature, stimulating the debate over the subject 6 . For example, the period during which the fibrin glue would keep NGF active in the repair still has to be defined.…”

Section: Discussionmentioning

confidence: 99%

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Nerve growth factor with fibrin glue in end-to-side nerve repair in rats

Silva

Aydos

et al. 2012

Acta Cir. Bras.

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PURPOSE:To determine the effects of end-to-side nerve repair performed only with fibrin glue containing nerve growth in rats. METHODS:Seventy two Wistar rats were divided into six equal groups: group A was not submitted to nerve section; group B was submitted to nerve fibular section only. The others groups had the nerve fibular sectioned and then repaired in the lateral surface of an intact tibial nerve, with different procedures: group C: ETS with sutures; group D: ETS with sutures and NGF; group E: ETS with FG only; group F: ETS with FG containing NGF. The motor function was accompanied and the tibial muscle mass, the number and diameter of muscular fibers and regenerated axons were measured. RESULTS:All the analyzed variables did not show any differences among the four operated groups (p>0.05), which were statistically superior to group B (p<0.05), but inferior to group A (p>0.05). CONCLUSION:The end-to-side nerve repair presented the same recovery pattern, independent from the repair used, showing that the addition of nerve growth factor in fibrin glue was not enough for the results potentiating.Key words: Suture Techniques. Fibrin Tissue Adhesive. Microsurgery. Rats. RESUMO OBJETIVO:Determinar os efeitos do reparo nervoso término-lateral realizado apenas com cola de fibrina contendo fator de crescimento nervoso em ratos. MÉTODOS:Setenta e dois ratos Wistar foram distribuídos em seis grupos: A -não submetido à secção nervosa; B -secção do nervo fibular (sem reparo); Os outros grupos tiveram o nervo fibular seccionado e então reparado na superfície lateral do nervo tibial

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Section: Many Authors Have Recently Enhanced the Results Of End-mentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Nerve growth factor with fibrin glue in end-to-side nerve repair in rats

Silva

Aydos

et al. 2012

Acta Cir. Bras.

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“…Tissue welding with lasers offers many advantages and has been studied in different settings during the last decades. The purpose of this study was to analyze the feasibility of a laser-mediated fixation of collagen scaffolds in comparison to clinically established methods [21].…”

Section: Discussionmentioning

confidence: 99%

Laser‐mediated fixation of collagen‐based scaffolds to dermal wounds

et al. 2010

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Background and Objective: Collagen scaffolds are popular for the reconstitution of dermal equivalents. Usually, these scaffolds are fixed with sutures or staples and in many cases these devices have to be removed in a second procedure. Laser-mediated tissue welding in a wet environment is a potential alternative for collagen scaffold fixation and may be advantageous to suture, staple, and tissue glue fixation. Materials and Methods: Welding was performed with a continuous-wave diode laser system emitting radiation at a wavelength of 968 nm. Tensile strength after fixation to porcine skin and laser parameters were determined in vitro. In vivo, 24 excisional deep partial thickness wounds were created on flanks of two Goettingen mini pigs and covered with collagen scaffolds. These were randomized and fixated with either (1) staples, (2) fibrin glue, or (3) laser-mediated welding. Tissue biopsies for histological analysis were periodically performed and analyzed for wound healing progression, epidermal thickness, and extracellular matrix formation. Results: Biomechanical stability after laser welding was time dependent. A dwell time of up to 10 seconds led to a strong bonding with a tensile strength of more than 30 g. In vivo, the wound healing process was macroscopically comparable in all groups and showed no significant differences. Microscopic analysis determined a more progressed and quicker wound closure in both the laser and staples group compared to the fibrin glue fixated scaffold. Laser-mediated fixation led to a significantly reduced epidermal thickness when compared with stapling or fibrin glue (P < 0.05). Conclusions: Laser tissue welding is a feasible approach for temporary fixation of collagen scaffolds to the wound bed. It improves wound healing properties and may lead to faster wound healing and cosmetically better scarring. Laser tissue welding is thus a very interesting and promising alternative to currently established fixation methods in a single step, no touch procedure

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“…Fibrin hydrogels harbor a great wound healing potential. They are among the most often used native hydrogels to induce angiogenesis and wound healing per se [25,26] and have been widely used as a drug delivery system [27,28].…”

Section: Introductionmentioning

confidence: 99%

The angiogenic response to PLL-g-PEG-mediated HIF-1α plasmid DNA delivery in healthy and diabetic rats

et al. 2013

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Impaired angiogenesis is a major clinical problem and affects wound healing especially in diabetic patients. Improving angiogenesis is a reasonable strategy to increase diabetes-impaired wound healing. Recently, our lab described a system of transient gene expression due to pegylated poly-l-lysine (PLL-g-PEG) polymer-mediated plasmid DNA delivery in vitro. Here we synthesized peptide-modified PLL-g-PEG polymers with two functionalities, characterized them in vitro and utilized them in vivo via a fibrin-based delivery matrix to induce dermal wound angiogenesis in diabetic rats. The two peptides were 1) a TG-peptide to covalently bind these nanocondensates to the fibrin matrix (TG-peptide) for a sustained release and 2) a polyR peptide to improve cellular uptake of these nanocondensates. In order to induce angiogenesis in vivo we condensed modified and non-modified polymers with plasmid DNA encoding a truncated form of the therapeutic candidate gene hypoxia-inducible transcription factor 1 (HIF-1 ). HIF-1 is the primarily oxygen-dependent regulated subunit of the heterodimeric transcription factor HIF-1, which controls angiogenesis among other physiological pathways. The truncated form of HIF-1 lacks the oxygen-dependent degradation domain (ODD) and therefore escapes degradation under normoxic conditions. PLL-g-PEG polymer-mediated HIF-1 -ΔODD plasmid DNA delivery was found to lead to a transiently induced gene expression of angiogenesis-related genes Acta2 and Pecam1 as well as the HIF-1 target gene Vegf in vivo. Furthermore, HIF-1 gene delivery was shown to enhance the number endothelial cells and smooth muscle cells -precursors for mature blood vessels -during wound healing. We show that -depending on the selection of the therapeutic target gene -PLL-g-PEG nanocondensates are a promising alternative to viral DNA delivery approaches, which might pose a risk to health. AbstractImpaired angiogenesis is a major clinical problem and affects wound healing especially in diabetic patients. Improving angiogenesis is a reasonable strategy to increase diabetes-impaired wound healing. Recently, our lab described a system of transient gene expression due to pegylated poly-L-lysine (PLL-g-PEG) polymermediated plasmid DNA delivery in vitro. Here we synthesized peptide-modified PLLg-PEG polymers with two functionalities, characterized them in vitro and utilized them in vivo via a fibrin-based delivery matrix to induce dermal wound angiogenesis in diabetic rats. The two peptides were 1) a TG-peptide to covalently bind these nanocondensates to the fibrin matrix (TG-peptide) for a sustained release and 2) a polyR peptide to improve cellular uptake of these nanocondensates. In order to induce angiogenesis in vivo we condensed modified and non-modified polymers with plasmid DNA encoding a truncated form of the therapeutic candidate gene hypoxiainducible transcription factor 1α (HIF-1α). HIF-1α is the primarily oxygen-dependent regulated subunit of the hetero-dimeric transcription factor HIF-1, which controls angiogenesis...

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The Use of Fibrin Glue in Skin Grafts and Tissue-Engineered Skin Replacements: A Review

Cited by 367 publications

References 90 publications

Nerve growth factor with fibrin glue in end-to-side nerve repair in rats

Nerve growth factor with fibrin glue in end-to-side nerve repair in rats

Laser‐mediated fixation of collagen‐based scaffolds to dermal wounds

The angiogenic response to PLL-g-PEG-mediated HIF-1α plasmid DNA delivery in healthy and diabetic rats

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