The use of in vitro fertilisation and embryo transfer techniques for the enhancement of genetic diversity in the captive population of the Lion-tailed macaque Macaca silenus

Collection and fertilization potential of sperm from the Sulawesi crested black macaque (Macaca nigra)

“…The sperm numbers we obtained from M. nigru were substantially less than those obtained by RPE from the rhesus monkey ( M . rnulattu) [Harrison, 1980;Gould & Mann, 19881, the stump-tailed macaque (Macucu urctoides) [Roussel & Austin, 19681, or the long-tailed macaque (Macucu fasciculuris) [ b u s s e l & Austin, 1968), but were close to sperm numbers obtained from the lion-tailed macaque (M. silenus) [Cranfield et al, 1988;Schaffer et al, 19891. We use a penile band technique for semen collection in the rhesus monkey and routinely recover samples of 300 x lo6 sperm or more [Lanzendorfet al, 19901.…”

In vitro fertilization‐embryo transfer in nonhuman primates: The technique and its applications

“…Acrosomal status has also been monitored but the ranking of freezing protocols remained unchanged (Tollner et al, 1990). The sperm penetration assay might provide a more rig- Roussel and Austin, 1967Roussel and Austin, 1967Roussel and Austin, 1967Roussel and Austin, 1967Leverage et al, 1972Cho and Honjo, 1973Cho et al, 1975Mahone and Dukelow, 1978Tollner et al, 1990Denis et al, 1976Cranfield et al, 1988Kraemer and Vera Cruz, 1969Roussel and Austin, 1967Gould and Styperek, 1989 freezer "Glycerol was used as cryoprotectant. Where multiple extenders were compared, only the most successful is included in the table. orous test of fertilizing ability (Gould and Styperek, 1989), but it is cumbersome and technically demanding.…”