The Use of Microorganisms in the Study of Fluorinated Compounds

Gottingen, Federal Republic of Germany 3-Chlorobenzoate-grown cells of Pseudomonas sp. B13 readily cometabolized monofluorobenzoates. A catabolic pathway for the isomeric fluorobenzoates is proposed on the basis of key metabolites isolated. Only 4-fluorobenzoate was utilized and totally degraded after a short period of adaptation. The isoenzymes for total degradation of chlorocatechols, being found during growth with 3chlorobenzoate or 4-chlorophenol, were not induced in the presence of fluorobenzoates. Correspondingly, only the ordinary enzymes of the benzoate pathway were detected in 4-fluorobenzoate-grown cells. Ring cleavage of 3-fluorocatechol was recognized as a critical step in 3-fluorobenzoate degradation. 2-Fluoro-cis,cismuconic acid was identified as a dead-end metabolite from 2-and 3-fluorobenzoate catabolism. During 2-fluorobenzoate cometabolism, fluoride is eliminated by the initial dioxygenation.

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Critical Reactions in Fluorobenzoic Acid Degradation by Pseudomonas sp. B13

Schreiber

Hellwig

Dorn

et al. 1980

Appl Environ Microbiol

104

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Anaerobic degradation of 2-fluorobenzoate by benzoate-degrading, denitrifying bacteria

Schennen¹,

Braun²,

Knackmuss³

1985

J Bacteriol

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Three strains of anaerobically benzoate-degrading, denitrifying bacteria of the genus Pseudomonas were able to grow on 2-fluorobenzoate as the sole carbon and energy souFce. Fluoride ion release was stoichiometric, and the reduction of dissolved organic carbon indicated total degradation. Cells grown anaerobically with benzoate were adapted for immediate growth with 2-fluorobenzoate, and both compounds were substrates for an inducible benzoyl-coenzyme A synthetase, the initial enzyme of anaerobic degradation. It is proposed that fluoride is eliminated gratuitously by a regioselective reaction in a sequence common to both carbon sources. Benzoate, but not 2.fluorobenzoate, was oxidized by aerobically grown cells.

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The Use of Microorganisms in the Study of Fluorinated Compounds

Cited by 2 publications

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Critical Reactions in Fluorobenzoic Acid Degradation by Pseudomonas sp. B13

Critical Reactions in Fluorobenzoic Acid Degradation by Pseudomonas sp. B13

Anaerobic degradation of 2-fluorobenzoate by benzoate-degrading, denitrifying bacteria

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