The use of mosquitoes (Aedes aegypti) to detect ciguatoxin in surgeon fishes (Ctenochaetus striatus)

Chungue, Eliane; Bagnis, R.; Parc, Francis

doi:10.1016/0041-0101(84)90150-8

Cited by 22 publications

(6 citation statements)

References 10 publications

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“…These tests are based on feeding cats, mongoose, chicken, crayfish, mosquito and dipteran larvae with flesh or viscera of suspect fish. Observations of symptoms, behaviour, growth, body temperature, and survival time of the animals over time are used to characterise and quantify toxin levels [ 165 – 170 ]. Of the 37 animal species tested by Banner et al [ 165 ], only five were found to be sensitive to the oral administration of ciguateric fish.…”

Section: Detection Of Ciguatoxinsmentioning

confidence: 99%

Ciguatoxins: Cyclic Polyether Modulators of Voltage-gated Iion Channel Function

2006

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Abstract:Ciguatoxins are cyclic polyether toxins, derived from marine dinoflagellates, which are responsible for the symptoms of ciguatera poisoning. Ingestion of tropical and subtropical fin fish contaminated by ciguatoxins results in an illness characterised by neurological, cardiovascular and gastrointestinal disorders. The pharmacology of ciguatoxins is characterised by their ability to cause persistent activation of voltage-gated sodium channels, to increase neuronal excitability and neurotransmitter release, to impair synaptic vesicle recycling, and to cause cell swelling. It is these effects, in combination with an action to block voltage-gated potassium channels at high doses, which are believed to underlie the complex of symptoms associated with ciguatera. This review examines the sources, structures and pharmacology of ciguatoxins. In particular, attention is placed on their cellular modes of actions to modulate voltage-gated ion channels and other Na + -dependent mechanisms in numerous cell types and to current approaches for detection and treatment of ciguatera.

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Section: Detection Of Ciguatoxinsmentioning

confidence: 99%

Ciguatoxins: Cyclic Polyether Modulators of Voltage-gated Iion Channel Function

2006

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“…Animal bioassay tests were developed during the 20th century. These tests were based on feeding cats [ 58 ], mongooses [ 59 ], chickens [ 60 ], or Dipteran larvae [ 61 ] with the flesh or viscera of suspected fish, or treating mosquitoes [ 62 ] or brine shrimp larvae [ 63 ] with fish extracts, and observing signs of intoxication and death of animals over time. Symptoms of cats and mongooses after being fed with toxic fish have been found to be similar in some respects to those of humans [ 59 ].…”

Section: Detection and Quantification Of Ciguatoxinsmentioning

confidence: 99%

“…The response of chickens to being fed contaminated fish liver has been found to be roughly quantitative. The mosquito bioassay test, where mosquitoes ( Aedes aegypti ) are intrathoracically injected, requires much smaller amounts of fish samples (8 g) than the tests discussed above, is much cheaper due to the low cost of mosquitoes, and is able to provide a LD 50 value in 2 h [ 62 ]. This test requires, however, a fish extract, therefore use of laboratory, as it is not practical to conduct the extraction procedure under field conditions.…”

Section: Detection and Quantification Of Ciguatoxinsmentioning

confidence: 99%

Advances in Detecting Ciguatoxins in Fish

Pasinszki

Lako

Dennis

2020

Toxins

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Ciguatera fish poisoning (CFP) is currently the most common marine biotoxin food poisoning worldwide, associated with human consumption of circumtropical fish and marine invertebrates that are contaminated with ciguatoxins. Ciguatoxins are very potent sodium-channel activator neurotoxins, that pose risks to human health at very low concentrations (>0.01 ng per g of fish flesh in the case of the most potent Pacific ciguatoxin). Symptoms of CFP are nonspecific and intoxication in humans is often misdiagnosed. Presently, there is no medically approved treatment of ciguatera. Therefore, to mitigate the risks of CFP, reliable detection of ciguatoxins prior to consumption of fish tissue is acutely needed, which requires application of highly sensitive and quantitative analytical tests. During the last century a number of methods have been developed to identify and quantify the concentration of ciguatoxins, including in vivo animal assays, cell-based assays, receptor binding assays, antibody-based immunoassays, electrochemical methods, and analytical techniques based on coupling of liquid chromatography with mass spectrometry. Development of these methods, their various advantages and limitations, as well as future challenges are discussed in this review.

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“…Selon Endean et al (1993), l'US est la masse minimale d'extrait de poissons (en g) nécessaire pour tuer une souris de 20 g dans les 24 h qui suivent son injection i.p.. Pour des raisons d'économie d'extrait, l'utilisation de la DL 50 (dose létale 50, i.e. la dose nécessaire à administrer pour obtenir une mortalité de 50 % des animaux) fut souvent substituée à celle de la dose létale minimale (DLM) (Chungue et al, 1984). D'autres auteurs (Vernoux, 1994 ; reprennent cette notion et l'expriment en équivalents-gramme de chair d'origine (US/g) rapportés à 1 g de souris (USg/g), valeur qui révèle la comestibilité de la chair pisciaire (une chair étant considérée comme comestible si sa toxicité est inférieure à 0,5 USg/g).…”

Section: Le « Test Souris » : Un Modèle De Référenceunclassified

Détection des ciguatoxines : avantages et inconvénients des différentes méthodes biologiques utilisées

et al. 2005

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Ciguatera is a seafood intoxication that results from ingestion of reef fish contaminated with ciguatoxins at levels orally toxic for humans. Precursors of those toxins, gambiertoxins, are produced by benthic dinoflagellates (genus Gambierdiscus), and then accumulated and biotransformed by herbivorous and carnivorous fishes into ciguatoxins, more toxic for humans. In the absence of specific treatment, that disease remains a health problem with otherwise adverse socio-economic impacts. Thus a cost-effective means of detecting ciguatoxins in fish has long been searched for. Many assays have been developed, including in vivo, in vitro, chemical or immunochemical approaches. This review focuses on some biological methods, from the well-standardised mouse assay to the specific radio-labelled ligand binding assay that is performed on rat brain synaptosomes. In addition to the mouse, the chick and the mongoose were still recently used, in particular for preliminary tests before ciguatoxin extraction from fish, since assays in these animals can directly assay the whole flesh. In contrast, various other in vivo methods, such as the kitten, mosquito and diptera larvae assays, were abandoned despite their interesting results. Finally, the mouse neuroblastoma and rat brain synaptosome assays, carried out in vitro as alternative approaches to animal-using assays, are highly sensitive and much more specific than the in vivo methods to detect ciguatoxins.

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The use of mosquitoes (Aedes aegypti) to detect ciguatoxin in surgeon fishes (Ctenochaetus striatus)

Cited by 22 publications

References 10 publications

Ciguatoxins: Cyclic Polyether Modulators of Voltage-gated Iion Channel Function

Ciguatoxins: Cyclic Polyether Modulators of Voltage-gated Iion Channel Function

Advances in Detecting Ciguatoxins in Fish

Détection des ciguatoxines : avantages et inconvénients des différentes méthodes biologiques utilisées

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