The use of nanovibration to discover specific and potent bioactive metabolites that stimulate osteogenic differentiation in mesenchymal stem cells

Abstract

Figure 2. Metabolic profiles of MSCs in 3D culture for 7 days with osteogenic media or nanovibration. (a) Heatmap (red = up-regulations, blue = down-regulations)and (b) principle component analysis depicting differences in nanovibration and OGM metabolic profiles during MSC differentiation. Metabolic profiles of MSCs induced by nanovibrational stimulation show a greater similarity to unstimulated controls at day 7 of culture and to OGM-stimulated cells at day 14. (c) Metabolic profiles were compared by Ingenuity Pathway Analysis (IPA). The metabolic profiles of nanovibrated cells lead to a stronger predicted activation of ERK1/2 relative to the metabolic profiles of cells induced by OGM. In contrast, the metabolic profiles of OGMstimulated cells lead to a stronger predicted down regulation of JNK, while those of nanovibrated cells predict JNK's activation rather than inhibition. Networks are built from d=1, r=4.

Nanozyme-reinforced hydrogel as a H2O2-driven oxygenerator for enhancing prosthetic interface osseointegration in rheumatoid arthritis therapy

“…Furthermore, the genes involved in osteogenic differentiation of BMSCs were determined by real-time quantitative polymerase chain reaction (RT-qPCR). The relative mRNA of osteogenic gene markers including alkaline phosphatase (ALP), bone morphogenetic protein-2 (BMP-2), runt-related transcription factor 2 (RUNX-2), and osteocalcin (OCN) were selected as representative examples 39,40 . According to Fig.…”

Extracellular vesicle cargo metabolome changes in response to the mesenchymal stromal cell microenvironment and influences cell quiescence and activation in a human breast cancer cell model

“…As discussed, bioactive metabolites are emerging as powerful signalling molecules that can drive cell processes (23, 73), as previously demonstrated in the control of stem cell differentiation (24,74,75). However, their role in cancer dormancy is not understood.…”

“…Metabolites can both act as bioactive compounds and fuel biological processes and, as such, are a growing focus of research (23,24,(73)(74)(75). We therefore assessed whether the pQ-EV metabolite cargo could cause a metabolic shift in MCF7 spheroids towards dormancy.…”

An ossifying landscape: materials and growth factor strategies for osteogenic signalling and bone regeneration

“…An interesting new strategy to induce osteogenesis utilizes a nanovibrational bioreactor which delivers ultra-precise vibrations to MSCs for guiding their differentiation into bone cells [16,67,68]. We have shown that stimulating cells with vibrations in the form of nano-meter amplitude results in elevated expression of osteogenic biomarkers such as osteocalcin, osteopontin, alkaline phosphatase and BMP-2.…”