The use of Nile Red to monitor the aggregation behavior in ternary surfactant–water–organic solvent systems

Stuart, Marc C. A.; Pas, JC van de; Engberts, Jbfn; Pas, J.C. van de

doi:10.1002/poc.919

Cited by 222 publications

(223 citation statements)

References 19 publications

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“…The solutions were vortexed and allowed to equilibrate overnight at 20 C. Subsequently, NR fluorescence was measured after vortexing and 10 min equilibration on a Varian Cary Eclipse Fluorescence Spectrophotometer (Agilent Technologies, Santa Clara, CA) using a 10 mm quartz cuvette, 5 nm excitation slit, 5 nm emission slit, 1 nm resolution, 0.5 s averaging time, 120 nm/min scan speed, and a Savitzky-Golay smoothing factor of 9. The temperature was controlled with a Peltier device at 20 C. SMA solutions with NR at different pH were excited at 490 nm because at that wavelength NR molecules in a more hydrophobic environment are selectively excited (18), which is convenient for the detection of a possible collapse of the polymer chain. SMA solutions prepared at different polymer concentrations to detect a possible CAC were excited at 550 nm, as mentioned in Stuart et al (18).…”

Section: Nile Red Fluorescence Experimentsmentioning

confidence: 99%

“…The temperature was controlled with a Peltier device at 20 C. SMA solutions with NR at different pH were excited at 490 nm because at that wavelength NR molecules in a more hydrophobic environment are selectively excited (18), which is convenient for the detection of a possible collapse of the polymer chain. SMA solutions prepared at different polymer concentrations to detect a possible CAC were excited at 550 nm, as mentioned in Stuart et al (18). The emission was recorded in the 550-700 nm region.…”

Section: Nile Red Fluorescence Experimentsmentioning

confidence: 99%

“…This fluorophore is solvatochromic, which means that its absorption and emission spectra are sensitive to the polarity of the environment in which it resides. In water, NR emits~660 nm with low intensity, but when it resides in a hydrophobic solvent like t-butanol, NR shows an emission of~620 nm with relatively high intensity (18). Thus, when SMA collapses into a conformation that contains hydrophobic domains, NR should be able to partition into those domains and show a blue shift in emission accompanied by an increase in intensity.…”

Section: The Number Of Charges Per Unit Length Of Polymer Determines mentioning

confidence: 99%

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Effect of Polymer Composition and pH on Membrane Solubilization by Styrene-Maleic Acid Copolymers

Scheidelaar

Koorengevel

Walree

et al. 2016

Biophysical Journal

129

170

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The styrene-maleic acid (SMA) copolymer is rapidly gaining attention as a tool in membrane research, due to its ability to directly solubilize lipid membranes into nanodisk particles without the requirement of conventional detergents. Although many variants of SMA are commercially available, so far only SMA variants with a 2:1 and 3:1 styreneto-maleic acid ratio have been used in lipid membrane studies. It is not known how SMA composition affects the solubilization behavior of SMA. Here, we systematically investigated the effect of varying the styrene/maleic acid on the properties of SMA in solution and on its interaction with membranes. Also the effect of pH was studied, because the proton concentration in the solution will affect the charge density and thereby may modulate the properties of the polymers. Using model membranes of 1,2-dimyristoyl-sn-glycero-3-phosphocholine lipids at pH > pH agg , we found that membrane solubilization is promoted by a low charge density and by a relatively high fraction of maleic acid units in the polymer. Furthermore, it was found that a collapsed conformation of the polymer is required to ensure efficient insertion into the lipid membrane and that efficient solubilization may be improved by a more homogenous distribution of the maleic acid monomer units along the polymer chain. Altogether, the results show large differences in behavior between the SMA variants tested in the various steps of solubilization. The main conclusion is that the variant with a 2:1 styrene-to-maleic acid ratio is the most efficient membrane solubilizer in a wide pH range.

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Section: Nile Red Fluorescence Experimentsmentioning

confidence: 99%

Section: Nile Red Fluorescence Experimentsmentioning

confidence: 99%

Section: The Number Of Charges Per Unit Length Of Polymer Determines mentioning

confidence: 99%

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Effect of Polymer Composition and pH on Membrane Solubilization by Styrene-Maleic Acid Copolymers

Scheidelaar

Koorengevel

Walree

et al. 2016

Biophysical Journal

129

170

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“…22 A solution of each dendron was prepared in PBS buffer at concentrations well above their CACs: 200 M for Chol-SS-G2 and 20 M for Chol 2 -SS-G2. Nile Red was then added and its fluorescence was determined as it resided in the hydrophobic interior of the self-assembled 65 nanostructure.…”

Section: Triggered Degradation Disassembly and Loss Of Selfassembledmentioning

confidence: 99%

Double-degradable responsive self-assembled multivalent arrays – temporary nanoscale recognition between dendrons and DNA

et al. 2014

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Article:Barnard, A., Posocco, P., Fermeglia, M. et This article reports self-assembling dendrons which bind DNA in a multivalent manner. The molecular design directly impacts on self-assembly which subsequently controls the way these multivalent nanostructures bind DNA -this can be simulated by multiscale modelling. Incorporation of an S-S linkage between the multivalent hydrophilic dendron and the hydrophobic units responsible for self-10 assembly allows these structures to undergo triggered reductive cleavage, with dithiothreitol (DTT) inducing controlled breakdown, enabling the release of bound DNA. As such, the high-affinity selfassembled multivalent binding is temporary. Furthermore, because the multivalent dendrons are constructed from esters, a second slow degradation step causes further breakdown of these structures. This two-step double-degradation mechanism converts a large self-assembling unit with high affinity for 15 DNA into small units with no measurable binding affinity -demonstrating the advantage of selfassembled multivalency (SAMul) in achieving highly responsive nanoscale binding of biological targets.

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“…13 In the presence of 3 the emission intensity of NR showed a significant increase together with a blue shift of the maximum emission wavelength (l max ) from 660 nm in pure water to 622 nm in aqueous solutions of 3 ([3] = 1.0 mM) (Fig. 1b and ESIw), indicative of the formation of hydrophobic domains.…”

mentioning

confidence: 98%

Size control and compartmentalization in self-assembled nano-structures of a multisegment amphiphile

et al. 2010

Self Cite

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A ''multisegment amphiphile'' has been synthesized by covalently connecting two well known building blocks, a gelator and a micelle forming surfactant. Self-assembly results in the formation of compartmentalized nano-object displaying properties inherited from both parents.Amphiphiles continue to attract widespread attention in science and technology, not only because of their fascinating self-assembly properties, but also because their numerous, diverse applications in e.g. detergent formulations, catalysis, and drug delivery. Despite their large chemical diversity, amphiphiles share a common structure consisting of a hydrophilic and a hydrophobic segment, giving rise to a limited range of morphologies like micelles, vesicles, bilayers, and the corresponding inverted phases. 1 More recently, other building blocks such as small molecule gelators and peptides have been used to construct novel self-assembled architectures like ribbons, helices and tubes 2 with regular, well-defined structural features at molecular length scales. 3 Nevertheless, small molecule based self-assembled structures cannot yet compete with the sophisticated architectures found in natural systems, especially with regard to the levels of compartmentalization, size control, and hierarchical structure formation. 4 Currently, such multicompartment structures of defined size and 3-50 nm structural features can most easily be fabricated by controlled phase separation of multiple covalently connected segments in block copolymers, giving rise to a variety of multicompartment assemblies 5 and hierarchically structured materials. 6 Only few examples have been reported of self-assembled structures from small molecules with a block structure. 7 The challenge remains to develop self-assembling systems based on small molecules that allow size control, hierarchical structure formation and compartmentalization at (sub)-molecular length scales. Previously we and others showed that the orthogonal self-assembly of certain hydrogelators and surfactants is a valid strategy towards new nanoarchitectures, including selfassembled interpenetrating networks and various organizations of vesicles or micelles with fibrous networks (Scheme 1a). 8,9 We anticipated that the covalent connection of two orthogonally self-assembling molecular building blocks would give rise to microphase separation phenomena at the molecular length scale.Here we present a first example of such a ''multisegment amphiphile'', as the small molecule counterpart of block copolymers (Scheme 1b). We found that this multisegment amphiphile self-assembles into well-defined aggregates with compartments and structural features at molecular length scales inherited from its parent compounds. Our results suggest that this behavior results from strong spatial constraints on a phase separating system at the molecular scale.A first requirement for a multisegment amphiphile is that the self-assembly of each segment is driven by incompatible interactions. In this study we have combined a low-molecularweight ...

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The use of Nile Red to monitor the aggregation behavior in ternary surfactant–water–organic solvent systems

Cited by 222 publications

References 19 publications

Effect of Polymer Composition and pH on Membrane Solubilization by Styrene-Maleic Acid Copolymers

Effect of Polymer Composition and pH on Membrane Solubilization by Styrene-Maleic Acid Copolymers

Double-degradable responsive self-assembled multivalent arrays – temporary nanoscale recognition between dendrons and DNA

Size control and compartmentalization in self-assembled nano-structures of a multisegment amphiphile

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