The use of probiotics, essential oils and fatty acids in the control of American foulbrood and other bee diseases

Kuzyšinová, Katarína; Mudroňová, Dagmar; Toporčák, J.; Molnár, Ladislav; Javorský, P.

doi:10.1080/00218839.2016.1252067

Cited by 23 publications

(16 citation statements)

References 58 publications

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“…Probiotics have been suggested as a possible tool for improving the resistance of honeybees to entomopathogenic bacteria ( Alippi & Reynaldi, 2006 ; Audisio et al, 2011 ; Evans & Armstrong, 2006 ; Killer et al, 2014 ; Kuzysinova et al, 2016 ), which provides an opportunity to test the effects of symbionts against M. plutonius ( Endo & Salminen, 2013 ; Killer et al, 2014 ; Wu et al, 2014 ). Rada et al (1997) isolated lactobacilli, enterococci, and some bifidobacteria during studies of honeybee gut microbiota; however, these researchers did not perform tests against pathogenic bacteria but only characterized B. asteroides using biochemical tests and analyzed the sensitivity of these bacteria to veterinary drugs.…”

Section: Discussionmentioning

confidence: 99%

Bacterial community associated with worker honeybees (Apis mellifera) affected by European foulbrood

Erban

Ledvinka

Kamler

et al. 2017

PeerJ

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BackgroundMelissococcus plutonius is an entomopathogenic bacterium that causes European foulbrood (EFB), a honeybee (Apis mellifera L.) disease that necessitates quarantine in some countries. In Czechia, positive evidence of EFB was absent for almost 40 years, until an outbreak in the Krkonose Mountains National Park in 2015. This occurrence of EFB gave us the opportunity to study the epizootiology of EFB by focusing on the microbiome of honeybee workers, which act as vectors of honeybee diseases within and between colonies.MethodsThe study included worker bees collected from brood combs of colonies (i) with no signs of EFB (EFB0), (ii) without clinical symptoms but located at an apiary showing clinical signs of EFB (EFB1), and (iii) with clinical symptoms of EFB (EFB2). In total, 49 samples from 27 honeybee colonies were included in the dataset evaluated in this study. Each biological sample consisted of 10 surface-sterilized worker bees processed for DNA extraction. All subjects were analyzed using conventional PCR and by metabarcoding analysis based on the 16S rRNA gene V1–V3 region, as performed through Illumina MiSeq amplicon sequencing.ResultsThe bees from EFB2 colonies with clinical symptoms exhibited a 75-fold-higher incidence of M. plutonius than those from EFB1 asymptomatic colonies. Melissococcus plutonius was identified in all EFB1 colonies as well as in some of the control colonies. The proportions of Fructobacillus fructosus, Lactobacillus kunkeei, Gilliamella apicola, Frischella perrara, and Bifidobacterium coryneforme were higher in EFB2 than in EFB1, whereas Lactobacillus mellis was significantly higher in EFB2 than in EFB0. Snodgrassella alvi and L. melliventris, L. helsingborgensis and, L. kullabergensis exhibited higher proportion in EFB1 than in EFB2 and EFB0. The occurrence of Bartonella apis and Commensalibacter intestini were higher in EFB0 than in EFB2 and EFB1. Enterococcus faecalis incidence was highest in EFB2.ConclusionsHigh-throughput Illumina sequencing permitted a semi-quantitative analysis of the presence of M. plutonius within the honeybee worker microbiome. The results of this study indicate that worker bees from EFB-diseased colonies are capable of transmitting M. plutonius due to the greatly increased incidence of the pathogen. The presence of M. plutonius sequences in control colonies supports the hypothesis that this pathogen exists in an enzootic state. The bacterial groups synergic to both the colonies with clinical signs of EFB and the EFB-asymptomatic colonies could be candidates for probiotics. This study confirms that E. faecalis is a secondary invader to M. plutonius; however, other putative secondary invaders were not identified in this study.

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Section: Discussionmentioning

confidence: 99%

Bacterial community associated with worker honeybees (Apis mellifera) affected by European foulbrood

Erban

Ledvinka

Kamler

et al. 2017

PeerJ

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“…In addition, two acyl-CoA Delta 11-desaturase-like genes related to fatty acid metabolism and biosynthesis of unsaturated fatty acids were significantly up-regulated after CSBV infection, while two long chain fatty acids genes were significantly down-regulated. It was reported that fatty acids were used to control the American foulbrood and other bee diseases of honeybee (Kuzyšinová et al, 2016). These evidences suggested that fatty acid metabolism and biosynthesis in host immune response could be critical factors to resistance CSBV.…”

Section: Discussionmentioning

confidence: 99%

Identification of Immune Response to Sacbrood Virus Infection in Apis cerana Under Natural Condition

Deng

Zhao²,

Shen

et al. 2020

Front. Genet.

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Chinese sacbrood virus (CSBV) is a serious threat to eastern honeybees (Apis cerana), especially larvae. However, the pathological mechanism of this deadly disease remains unclear. Here, we employed mRNA and small RNA (sRNA) transcriptome approach to investigate the microRNAs (miRNAs) and small interfering RNAs (siRNAs) expression changes of A. cerana larvae infected with CSBV under natural condition. We found that serine proteases involved in immune response were down-regulated, while the expression of siRNAs targeted to serine proteases were up-regulated. In addition, CSBV infection also affected the expression of larvae cuticle proteins such as larval cuticle proteins A1A and A3A, resulting in increased susceptibility to CSBV infection. Together, our results provide insights into sRNAs that they are likely to be involved in regulating honeybee immune response.

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“…Several natural compounds were studied for antagonistic activity against P. larvae in vitro [ 57 , 58 , 59 ], however, the identified cytotoxic effects on bees had limited their practical application. Alternatives, such as prevention and control methods of the AFB pathogen are an area of great interest.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antagonistic Effect of Gut Bacteriota Isolated from Indigenous Honey Bees and Essential Oils against Paenibacillus Larvae

Kačániová

Terentjeva

Žiarovská

et al. 2020

IJMS

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The aim of study was to isolate and identify the gut bacteria of Apis mellifera and to evaluate antagonistic effect of the bacteriota against Paenibacillus larvae, which causes American foulbrood (AFB) in honeybees. The dilution plating method was used for the quantification of selected microbial groups from digestive tract of bees, with an emphasis on the bacteriota of the bees’ intestines. Bacteria were identified using mass spectrometry (MALDI-TOF-MS Biotyper). Overall, five classes, 27 genera and 66 species of bacteria were identified. Genera Lactobacillus (10 species) and Bacillus (8 species) were the most abundant. Gram-negative bacteria were represented with 16 genera, whereas Gram-positive with 10 genera. Delftia acidovorans and Escherichia coli were the most abundant in the digestive tract of honey bee. Resistance to a selection of antimicrobials was assessed for the bacterial isolates from bee gut and confirmed against all antimicrobials included in the study, with the exception of cefepime. Lactobacillus spp., especially L. kunkeei, L. crispatus and L. acidophilus. showed the strongest antimicrobial activity against P. larvae, the causal pathogen of AFB. Antimicrobial activity of essential oils against isolated bacteria and two isolates of P. larvae were assessed. Application of a broad selection of plant essential oils indicated that Thymus vulgaris had the highest antimicrobial activity against P. larvae.

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The use of probiotics, essential oils and fatty acids in the control of American foulbrood and other bee diseases

Cited by 23 publications

References 58 publications

Bacterial community associated with worker honeybees (Apis mellifera) affected by European foulbrood

Bacterial community associated with worker honeybees (Apis mellifera) affected by European foulbrood

Identification of Immune Response to Sacbrood Virus Infection in Apis cerana Under Natural Condition

In Vitro Antagonistic Effect of Gut Bacteriota Isolated from Indigenous Honey Bees and Essential Oils against Paenibacillus Larvae

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