The Use of Scaffolds in Cartilage Regeneration

Kalkan, Rasime; Nwekwo, Chidi Wilson; Adalı, Terin

doi:10.1615/critreveukaryotgeneexpr.2018024574

Cited by 24 publications

(35 citation statements)

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“…Therefore, scaffolds must possess adequate parameters, such as correct architecture, biocompatibility, degradability, or specific chemical and physical properties. This can be achieved through the choice of appropriate materials, additives, such as pore precursors, and manufacturing methods [ 17 , 57 ].…”

Section: Scaffold For Articular Cartilage Repair: Requirements Mamentioning

confidence: 99%

“…Scaffolds for cartilage tissue engineering should provide an appropriate environment and enable cell adhesion, migration, and development by having an appropriate architecture, controlled degradability, adequate mechanical parameters, and good biocompatibility. Many structural features, including porosity, pore size, interconnectivity, and permeability, play a meaningful role in AC development and cartilage regeneration [ 15 , 17 , 51 , 57 ]. A three-dimensional design for scaffolds is necessary to prevent the dedifferentiation of chondrocytes into fibroblast-like cells or the chondrogenesis of MSCs [ 50 , 58 , 59 , 60 ].…”

Section: Scaffold For Articular Cartilage Repair: Requirements Mamentioning

confidence: 99%

“…Scaffolds should maintain appropriate parameters in their stiffness, strength, and flexibility, conducive to integration and further tissue development. These parameters are important during cultivation and especially after implantation into the body due to the conditions in the knee [ 15 , 19 , 44 , 50 , 51 , 57 , 58 , 59 , 63 , 64 , 65 ]. Moreover, the parameters of scaffolds should be adapted to the types of cells.…”

Section: Scaffold For Articular Cartilage Repair: Requirements Mamentioning

confidence: 99%

“…Therefore, appropriate materials for the production of scaffolds should be selected. Such materials can be made of synthetic or natural polymers or a combination of both (i.e., hybrid materials (hybrid)) [ 17 , 25 , 51 , 57 , 64 , 76 ]. Natural materials such as collagen [ 64 , 69 , 77 ], hyaluronic acid (HA) [ 78 , 79 ], chitosan (CH) [ 80 , 81 ], chondroitin sulfate (CS) [ 82 , 83 ], and fibrin [ 25 , 84 , 85 ] are widely used in the production of scaffolds for cartilage regeneration.…”

Section: Scaffold For Articular Cartilage Repair: Requirements Mamentioning

confidence: 99%

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Review of Synthetic and Hybrid Scaffolds in Cartilage Tissue Engineering

2020

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Cartilage tissue is under extensive investigation in tissue engineering and regenerative medicine studies because of its limited regenerative potential. Currently, many scaffolds are undergoing scientific and clinical research. A key for appropriate scaffolding is the assurance of a temporary cellular environment that allows the cells to function as in native tissue. These scaffolds should meet the relevant requirements, including appropriate architecture and physicochemical and biological properties. This is necessary for proper cell growth, which is associated with the adequate regeneration of cartilage. This paper presents a review of the development of scaffolds from synthetic polymers and hybrid materials employed for the engineering of cartilage tissue and regenerative medicine. Initially, general information on articular cartilage and an overview of the clinical strategies for the treatment of cartilage defects are presented. Then, the requirements for scaffolds in regenerative medicine, materials intended for membranes, and methods for obtaining them are briefly described. We also describe the hybrid materials that combine the advantages of both synthetic and natural polymers, which provide better properties for the scaffold. The last part of the article is focused on scaffolds in cartilage tissue engineering that have been confirmed by undergoing preclinical and clinical tests.

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Section: Scaffold For Articular Cartilage Repair: Requirements Mamentioning

confidence: 99%

Section: Scaffold For Articular Cartilage Repair: Requirements Mamentioning

confidence: 99%

Section: Scaffold For Articular Cartilage Repair: Requirements Mamentioning

confidence: 99%

Section: Scaffold For Articular Cartilage Repair: Requirements Mamentioning

confidence: 99%

See 2 more Smart Citations

Review of Synthetic and Hybrid Scaffolds in Cartilage Tissue Engineering

2020

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“…Tissue engineering techniques are focused on developing biological substitutes to replace the damaged AC (Johnstone et al, 2013;Kalkan, Nwekwo, & Adali, 2018). Biomaterials as solid porous and viscoelastic polymers (hydrogels) have been fabricated and evaluated for its use in cartilage regeneration (DeKosky et al, 2010;Duarte Campos, Drescher, Rath, Tingart, & Fischer, 2012;Vinatier & Guicheux, 2016).…”

Section: Introductionmentioning

confidence: 99%

Chitosan‐based scaffold counteracts hypertrophic and fibrotic markers in chondrogenic differentiated mesenchymal stromal cells

Manferdini

Gabusi

Sartore

et al. 2019

J Tissue Eng Regen Med

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Cartilage tissue engineering remains problematic because no systems are able to induce signals that contribute to native cartilage structure formation. Therefore, we tested the potentiality of gelatin‐polyethylene glycol scaffolds containing three different concentrations of chitosan (CH; 0%, 8%, and 16%) on chondrogenic differentiation of human platelet lysate‐expanded human bone marrow mesenchymal stromal cells (hBM‐MSCs). Typical chondrogenic (SOX9, collagen type 2, and aggrecan), hypertrophic (collagen type 10), and fibrotic (collagen type 1) markers were evaluated at gene and protein level at Days 1, 28, and 48. We demonstrated that 16% CH scaffold had the highest percentage of relaxation with the fastest relaxation rate. In particular, 16% CH scaffold, combined with chondrogenic factor TGFβ3, was more efficient in inducing hBM‐MSCs chondrogenic differentiation compared with 0% or 8% scaffolds. Collagen type 2, SOX9, and aggrecan showed the same expression in all scaffolds, whereas collagen types 10 and 1 markers were efficiently down‐modulated only in 16% CH. We demonstrated that using human platelet lysate chronically during hBM‐MSCs chondrogenic differentiation, the chondrogenic, hypertrophic, and fibrotic markers were significantly decreased. Our data demonstrate that only a high concentration of CH, combined with TGFβ3, creates an environment capable of guiding in vitro hBM‐MSCs towards a phenotypically stable chondrogenesis.

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Photo‐Cross‐Linkable, Injectable, and Highly Adhesive GelMA‐Glycol Chitosan Hydrogels for Cartilage Repair

Paul,

Schrobback,

Tran

et al. 2023

Adv Healthcare Materials

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Hydrogels provide a promising platform for cartilage repair and regeneration. Although hydrogels have shown some efficacy, they still have shortcomings including poor mechanical properties and suboptimal integration with surrounding cartilage. Herein, we develop hydrogels that are injectable, cytocompatible, mechanically robust, and highly adhesive to cartilage. Our approach uses GelMA‐glycol chitosan (GelMA‐GC) that is crosslinkable with visible light and photo‐initiators (lithium acylphosphinate (LAP) and tris (2,2′‐bipyridyl) dichlororuthenium (II) hexahydrate ([RuII(bpy)3]2+ and sodium persulfate (Ru/SPS)). Ru/SPS‐cross‐linked hydrogels have higher compressive and tensile modulus, and most prominently higher adhesive strength with cartilage, which also depends on inclusion of GC. Tensile and push‐out tests of the Ru/SPS‐cross‐linked GelMA‐GC hydrogels demonstrate adhesive strength of approximately 100 kPa and 46 kPa, respectively. Hydrogel precursor solutions behave in a Newtonian manner and are injectable. After injection in focal bovine cartilage defects and in situ cross‐linking, this hydrogel system remains intact and integrated with cartilage following joint manipulation ex vivo. Cells remain viable (> 85%) in the hydrogel system, and further show tissue regeneration potential after three weeks of in vitro culture. These preliminary results provide further motivation for future research on bioadhesive hydrogels for cartilage repair and regeneration.This article is protected by copyright. All rights reserved

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The Use of Scaffolds in Cartilage Regeneration

Cited by 24 publications

References 0 publications

Review of Synthetic and Hybrid Scaffolds in Cartilage Tissue Engineering

Review of Synthetic and Hybrid Scaffolds in Cartilage Tissue Engineering

Chitosan‐based scaffold counteracts hypertrophic and fibrotic markers in chondrogenic differentiated mesenchymal stromal cells

Photo‐Cross‐Linkable, Injectable, and Highly Adhesive GelMA‐Glycol Chitosan Hydrogels for Cartilage Repair

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