The use of separation techniques in the analysis of some antiepileptic drugs: A critical review

Mohamed, Fardous A.; Bakr, Marwa F.; Rageh, Azza H.; Mostafa, Aya M.

doi:10.1080/10826076.2016.1266654

Cited by 10 publications

(3 citation statements)

References 59 publications

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“…Optimal separation for all analytes tested was achieved on the Luna Phenyl-Hexyl column by gradient elution using a mixture of potassium phosphate buffer (25 mM, pH 5.1) and methanol as the mobile phase with a flow rate of 1 mL/min at 210 nm. To the best of our knowledge, this is the first HPLC-UV method using a Phenyl-Hexyl column to separate AEDs, as other published methods use C18 columns and acetonitrile as an organic modifier [5][6][7]. The instrumentation and chromatographic conditions are described in detail in Section 3.2.…”

Section: Optimization Of the Analytical Methodsmentioning

confidence: 99%

“…To date, several methods based on high-performance liquid chromatography (HPLC) coupled to ultraviolet or mass spectrometry detection for the simultaneous quantification of AEDs in human plasma or serum have been reported and reviewed elsewhere [5][6][7][8]. Some of the methods also include quantification of the main metabolites, such as 10-monohydroxycarbazepine (MHD) and carbamazepine-10,11-epoxide (CBZ-E), pharmacologically active metabolites of OXC and CBZ, respectively [9][10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

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Simple HPLC-UV Method for Therapeutic Drug Monitoring of 12 Antiepileptic Drugs and Their Main Metabolites in Human Plasma

Milosheska,

Roškar

2023

Molecules

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The objective of the present report was to develop and validate a simple, selective, and reproducible high-performance liquid chromatography method with UV detection suitable for routine therapeutic drug monitoring of the most commonly used antiepileptic drugs and some of their metabolites. Simple precipitation of plasma proteins with acetonitrile was used for sample preparation. 10,11-dihydrocarbamazepine was used as an internal standard. Chromatographic separation of the analytes was achieved by gradient elution on a Phenyl–Hexyl column at 40 °C, using methanol and potassium phosphate buffer (25 mM; pH 5.1) as a mobile phase. The method was validated according to the FDA guidelines for bioanalytical method validation. It showed to be selective, accurate, precise, and linear over the concentration ranges of 1–50 mg/L for phenobarbital, phenytoin, levetiracetam, rufinamide, zonisamide, and lacosamide; 0.5–50 mg/L for lamotrigine, primidone, carbamazepine and 10-monohydroxycarbazepine; 0.2–10 mg/L for carbamazepine metabolites: 10,11-trans-dihydroxy-10,11-dihydrocarbamazepine and carbamazepine-10,11-epoxide; 0.1–10 mg/L for oxcarbazepine; 2–100 mg/L for felbamate and 3–150 mg/L for ethosuximide. The suitability of the validated method for routine therapeutic drug monitoring was confirmed by quantification of the analytes in plasma samples from patients with epilepsy on combination antiepileptic therapy.

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Section: Optimization Of the Analytical Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simple HPLC-UV Method for Therapeutic Drug Monitoring of 12 Antiepileptic Drugs and Their Main Metabolites in Human Plasma

Milosheska,

Roškar

2023

Molecules

View full text Add to dashboard Cite

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“…Pregabalin: 2016 Direct Separation of Pregabalin enantiomers using a Zwitterionic chiral selector and analysis by two HPLC methods including detection by MS and UV [ 394 ]; results of a national study of Pregabalin abuse in France [ 395 ]; preparation and evaluation of floating tablets of pregabalin prepared in differing concentrations of xanthan and guar gum [ 396 ]; review article on the use of separation techniques including HP-TLC, HPLC, GC and electrophoresis in the determination of antiepileptic drugs including pregabalin (also includes eslicarbazepine acetate, levetiracetam, lacosamide, oxcarbazepine, and retigabine) [ 397 ]; study of the abuse potential of pregabalin [ 398 , 399 ]; validated fluorometric UHPLC method to measure pregabalin [ 400 ]; Thermomyces lanuginosus lipase for the efficient production of (S)-2-carboxyethyl-3-cyano-5-methylhexaoic acid used as chiral intermediate for pregabalin [ 401 ]; 2017 gabapentin diversion and misuse from 2002-2015 based on law enforcement-derived data [ 402 ]; abuse and misuse of Pregabalin and Gabapentin [ 403 ]; Pregabalin misuse and abuse reported to US Poison Centers [ 404 ]; synthesis of (+/−)-Pregabalin by using a three-step sequential-flow system with heterogeneous catalysts [ 405 ]; survey of the use of pregabalin among users of illicit drugs in Southern Germany [ 406 ]; systematic review of the effectiveness of policies restricting access to pregabalin [ 407 ]; Pregabalin abuse in Munich [ 408 , 409 ]; 2018 literature review of the abuse potential of pregabalin [ 410 ]; formulation of controlled-release tablets containing 150 mg pregabalin [ 411 ]; pregabalin immediate release tablets were prepared by direct compression method using central composite design with response surface methodology [ 412 ]; Preparation and evaluation of non-effervescent tablets containing pregabalin [ 413 ]; synthesis method of racemic Pregabalin, Baclofen and 3-Phenibut involving Lossen rearrangement [ 414 ]; Pregabalin misuse in methadone maintenance treatment patients in Israel [ 415 ]; 2019 validated GC/MS for the evaluation and quantification of pregabalin in pharmaceutical preparations [ 416 ];…”

Section: Routine and Improved Analyses Of Abused Substancesmentioning

confidence: 99%