The Use of Sodium Taurodeoxycholate for Excystation of Eimeria tenella Sporozoites

Patton, W. H.; Brigman, G. P.

doi:10.2307/3280315

Cited by 20 publications

(4 citation statements)

References 12 publications

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“…The in vitro excystation procedure was a modification of a previously reported method for excystation of Eimeria tenella (22). Aliquots (200 l) of control and disinfectant-treated oocysts (ϳ2 ϫ 10 6 ) were suspended in 500 l HBSS, pH 7.2, containing 0.25% (wt/vol) bovine trypsin and 0.8% (wt/vol) sodium taurodeoxycholate and incubated for 90 min at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Efficacy of Two Peroxygen-Based Disinfectants for Inactivation of Cryptosporidium parvum Oocysts

Quílez

Sánchez-Acedo

Avendaño

et al. 2005

Appl Environ Microbiol

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Two commercial peroxygen-based disinfectants containing hydrogen peroxide plus either peracetic acid (Ox-Virin) or silver nitrate (Ox-Agua) were tested for their ability to inactivate Cryptosporidium parvum oocysts. Oocysts were obtained from naturally infected goat kids and exposed to concentrations of 2, 5, and 10% Ox-Virin or 1, 3, and 5% Ox-Agua for 30, 60, and 120 min. In vitro excystation, vital dyes (4,6-diamidino-2-phenylindole and propidium iodide), and infectivity in neonatal BALB/c mice were used to assess the viability and infectivity of control and disinfectant-treated oocysts. Both disinfectants had a deleterious effect on the survival of C. parvum oocysts, since disinfection significantly reduced and in some cases eliminated their viability and infectivity. When in vitro assays were compared with an infectivity assay as indicators of oocyst inactivation, the excystation assay showed 98.6% inactivation after treatment with 10% Ox-Virin for 60 min, while the vital-dye assay showed 95.2% inactivation and the infectivity assay revealed 100% inactivation. Treatment with 3% Ox-Agua for 30 min completely eliminated oocyst infectivity for mice, although we were able to observe only 74.7% inactivation as measured by excystation assays and 24.3% with vital dyes (which proved to be the least reliable method for predicting C. parvum oocyst viability). These findings indicate the potential efficacy of both disinfectants for C. parvum oocysts in agricultural settings where soil, housing, or tools might be contaminated and support the argument that in comparison to the animal infectivity assay, vital-dye and excystation methods overestimate the viability of oocysts following chemical disinfection.

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Section: Methodsmentioning

confidence: 99%

Efficacy of Two Peroxygen-Based Disinfectants for Inactivation of Cryptosporidium parvum Oocysts

Quílez

Sánchez-Acedo

Avendaño

et al. 2005

Appl Environ Microbiol

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“…All dilutions of the stock pMIMs, sporozoites, and merozoites were made in Saline A. Sporozoites of Eimeria acervulina (PBEL isolate 12), E. brunetti (PBEL isolate 32), E. maxima (PBEL isolate 68), E. necatrix (PBEL isolate 56), and E. tenella (PBEL isolate 80) were freshly excysted (Patton and Brigman 1979). Merozoites were obtained fresh from intestinal tissue of infected chickens (Jenkins and Dame 1987).…”

Section: In Vitro Studies On Lysismentioning

confidence: 99%

Evaluation of the effect of peptidyl membrane-interactive molecules on avian coccidia

Martin

Danforth

Jaynes

et al. 1999

Parasitology Research

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This study examined the lytic effect of seven different synthetic peptidyl membrane-interactive molecules (Peptidyl-MIMs) on sporozoites of five different species of Eimeria infecting chickens and merozoites of two different species that infect chickens. All Peptidyl-MIMs (pMIMs) demonstrated antiparasitic effects at concentrations of 1-50 microM during incubation periods varying from 1 to 20 min. In addition, electron microscopy showed that ultrastructural degeneration of the pellicle of sporozoite stages of the parasites occurred within 5-10 min of exposure to 5-microM concentrations of three different pMIMs. Pore-like openings were seen in the pellicle of the sporozoites at the ultrastructural level, which indicated that the pMIMs had the same mechanism of action on the parasites as that reported from studies done on bacteria. A reduction in lesion scores was seen in chickens treated orally with 10-, 50-, or 75-microM concentrations of two different proteolytic stabilized (methylated) pMIMs after challenge with three different species of avian coccidia in battery-cage trials. Collectively these data indicate that pMIMs may be useful in the control of coccidiosis in poultry.

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“…Numbers of oocysts per given volume were determined by the procedure described by Roberson et al (1973). Excystation of sporocysts was done by the method of Patton & Brigman (1979), and sporozoites were isolated by the method of Wagenbach (1969), with a glass-bead column (2.5 cm x 16 cm). Sporozoites were suspended in 0.02 M-Tris/HCl buffer, pH 7.4, and sonicated for two periods of 2 min at 5 min intervals at 0°C with a sonic Dismembrator model 300 (Fisher) operating at full power ).…”

Section: Purification Of Chicken Caecal Arylsulphatasementioning

confidence: 99%

Comparison of arylsulphatases from Eimeria tenella (parasite) and chicken caecum (host)

Farooqui¹,

Hanson²

1987

Biochemical Journal

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Chicken caecal arylsulphatase was purified 700-fold by (NH4)2SO4 fractionation, concanavalin A-Sepharose and cyclic AMP-Sepharose chromatographies. The purified enzyme was a glycoprotein of Mr 97,000. It hydrolysed p-nitrocatechol sulphate, cerebroside 3-sulphate and ascorbic acid 2-sulphate and was strongly inhibited by Na2SO4 (Ki = 50 microM) and Na3PO4 (Ki = 20 microM). Arylsulphatase from Eimeria tenella sporozoites was purified 28-fold by (NH4)2SO4 fractionation. Arylsulphatase of E. tenella sporozoites was not a glycoprotein. It had an Mr of 49,000. It was inhibited by Na2SO4 (Ki = 300 microM), sodium phosphate (Ki = 90 microM) and heparin. It hydrolysed ascorbic acid 2-sulphate, but cerebroside 3-sulphate was not desulphated. The kinetic parameters of chicken caecal arylsulphatase were different from those of the E. tenella enzyme.

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The Use of Sodium Taurodeoxycholate for Excystation of Eimeria tenella Sporozoites

Cited by 20 publications

References 12 publications

Efficacy of Two Peroxygen-Based Disinfectants for Inactivation of Cryptosporidium parvum Oocysts

Efficacy of Two Peroxygen-Based Disinfectants for Inactivation of Cryptosporidium parvum Oocysts

Evaluation of the effect of peptidyl membrane-interactive molecules on avian coccidia

Comparison of arylsulphatases from Eimeria tenella (parasite) and chicken caecum (host)

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