The use of spa and phage typing for characterization of a MRSA population in a Belgian hospital: Comparison between 2002 and 2007

Wildemauwe, Christa; Brouwer, D. De; Godard, C.; Buyssens, P.; Dewit, J; Joseph, R. L.; Vanhoof, R.

doi:10.1016/j.patbio.2009.07.024

Cited by 8 publications

(8 citation statements)

References 15 publications

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“…Molecular typing is thus essential to determine the origin of the isolates, its clonal relationship and also for epidemiological investigations that play an important role in the prevention and treatment of infections. [ 22 ] The present study confirmed the presence of 209 (80.7%) MRSA isolates which is found to be much higher than previous findings. [ 6 16 ] The antibiotic resistance rates of MRSA to various classes of antibiotics used in this study were higher than the global average.…”

Section: Discussionsupporting

confidence: 85%

Genotyping of methicillin resistant staphylococcus aureus from tertiary care hospitals in Coimbatore, South India

Neetu

Sevanan

2016

J Global Infect Dis

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Background:Globally, methicillin resistant Staphylococcus aureus (MRSA) is one of the most common pathogens that causes hospital- and community-acquired infections. The use of molecular typing methods is essential for determining the origin of the isolates, their clonal relations, and also epidemiological investigations.Objective:The purpose of this study was to determine the prevalence of antibiotic-resistant MRSA investigate the accessory gene regulator (agr) and staphylococcal cassette chromosome mec (SCCmec) types and perform multilocus sequence typing (MLST). Furthermore, the minimum inhibitory concentration of MRSA isolates was determined for vancomycin and daptomycin.Materials and Methods:Two hundred and fifty-nine MRSA isolates were collected from Tertiary Care Hospitals in Coimbatore. Disk diffusion method was employed to assess the sensitivity of MRSA isolates to selected antibiotics and genetic analysis was performed using SCCmec, agr, and MLST typing by multiplex-polymerase chain reaction strategy. Minimal inhibitory concentration (MIC) was determined using Ezy MIC (vancomycin) and Biomerieux (daptomycin) E-test strip.Results:Of 259 MRSA isolates, 209 (80.7%) were confirmed as methicillin resistant. Antibiotic susceptibility pattern revealed that all the MRSA isolates were 100% sensitive to linezolid, rifampicin, teicoplanin, and vancomycin. MIC results showed that of 209 MRSA isolates, 10 were found to be vancomycin intermediate S. aureus and 100% of the MRSA isolates were daptomycin-susceptible. The agr group I and SCCmec Type III were the major type among MRSA isolates. In addition to these MLST typing revealed the prevalence of sequence type (ST) 239 (SLV of ST8) among the MRSA isolates.Conclusion:This study confirms that ST239 (Brazilian clone) of MRSA is predominant in this region which is responsible for the hospital-acquired MRSA infections. Thus, the study also suggests that vancomycin and daptomycin can still be used as an alternative drug for treating severe MRSA infections.

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Section: Discussionsupporting

confidence: 85%

Genotyping of methicillin resistant staphylococcus aureus from tertiary care hospitals in Coimbatore, South India

Neetu

Sevanan

2016

J Global Infect Dis

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“…SCCmec typing provides useful information about the resistance of genes to methicillin and to identify the origin of strains [28,29]. In our study, SCCmec typeIII was the most common type, which was similar to other reports in Iran and other countries [11,18,22,26,27,30,31].…”

Section: Discussionsupporting

confidence: 88%

Characterization of SCCmec, spa types and Multi Drug Resistant of meticillin-resistant Staphylococcus aureus isolates among inpatients and outpatients in a referral hospital in Shiraz, Iran.

Hashemizadeh

Hadi

Mohebi

et al. 2019

Preprint

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Objectives: Molecular typing such as spa typing is used to control and prevent of Staphylococcus. aureus widespread in hospitals and community acquired. Hence, the aim of this study was to find the common types of S. aureus strains circulating in Shiraz via spa and SCCmec typing methods. Results: Total of 159 S. aureus isolates were collected from two tertiary hospitals in Shiraz. Isolates were identified by biochemical tests. Antimicrobial susceptibility tests were performed by standard disk diffusion method and then genetic analysis of bacteria was performed using SCCmec and spa typing. In this study 31.4% of the isolates were methicillin-resistant S. aureus. The majority of isolates were SSC mec type ІІІ. Spa type t030 was the most prominent type among MRSA strains. In this study, for the first time in Iran, spa003, t386, t1877, t314, t186, t1816, t304, t325, t345 were reported. It was shown that there is a possibility that these spa types are natives to this region. Our findings showed that SCCmec ІІ, ІІІ and IV disseminate from hospital to community and vice versa. Thus, effective monitoring of MRSA in hospital and community is desirable. Keyword: Staphylococcus aureus , methicillin-resistant S. aureus (MRSA), SCCmec typing, spa typing

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“…Those three types are usually identified among hospital-acquired (HA)-MRSA. However, while MRSA spa type t121 was uncommonly found in Belgian hospitals [37] it has been commonly recovered in hospitals in Europe and in the United States [38]. This spa type has also been found in bulk tank milk in the United States [39].…”

Section: Discussionmentioning

confidence: 99%

Epidemiology and molecular characterization of methicillin-resistant Staphylococcus aureus nasal carriage isolates from bovines

et al. 2014

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BackgroundStaphylococcus aureus is a common bacterium usually found on skin and mucous membranes of warm blooded animals. Resistance in S. aureus has been increasingly reported though depending on the clonal lineage. Indeed, while hospital acquired (HA)-methicillin resistant S. aureus (MRSA) are typically multi-resistant, community associated (CA)-MRSA are by large more susceptible to many antibiotics. Although S. aureus isolated from animals are often susceptible to most antibiotics, multi-resistant livestock associated (LA)-MRSA have been recovered from bovine mastitis.In this study, we investigated the prevalence and types of MRSA present in the nose of healthy bovines of different age groups and rearing practices. Since no validated methods for MRSA isolation from nasal swabs were available, we compared two isolation methods. Molecular characterization was performed by means of spa-typing, MLST, SCCmec typing and microarray analysis for the detection of antimicrobial resistance and virulence genes.ResultsMRSA between herd prevalence in bovines was estimated at 19.8%. There was a marked difference between rearing practices with 9.9%, 10.2% and 46.1% of the dairy, beef and veal calve farms respectively being MRSA positive. No significant difference was observed between both isolation methods tested. Most isolates were ST398 spa type t011 or closely related spa types. Few ST239 spa type t037 and t388 and ST8 spa type t121 were also found. SCCmec types carried by these strains were mainly type IV(2B), IV(2B&5) and type V. Type III and non-typeable SCCmec were recovered to a lesser extent. All isolates were multi-resistant to at least two antimicrobials in addition to the expected cefoxitin and penicillin resistance, with an average of resistance to 9.5 different antimicrobials. Isolates selected for microarray analysis carried a broad range of antimicrobial resistance and virulence genes.ConclusionMRSA were mainly present in veal farms, compared to the lower prevalence in dairy or beef farms. Multi-resistance in these strains was high. Though mainly CC398 spa t011 was found, the genetic diversity was higher than what was found for pigs in Belgium. CC8 strains, a typically human lineage but also recently found also in association with bovines, has been retrieved here also.

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The use of spa and phage typing for characterization of a MRSA population in a Belgian hospital: Comparison between 2002 and 2007

Cited by 8 publications

References 15 publications

Genotyping of methicillin resistant staphylococcus aureus from tertiary care hospitals in Coimbatore, South India

Genotyping of methicillin resistant staphylococcus aureus from tertiary care hospitals in Coimbatore, South India

Characterization of SCCmec, spa types and Multi Drug Resistant of meticillin-resistant Staphylococcus aureus isolates among inpatients and outpatients in a referral hospital in Shiraz, Iran.

Epidemiology and molecular characterization of methicillin-resistant Staphylococcus aureus nasal carriage isolates from bovines

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