The Use of Stimulable Bioluminescence from Marine Dinoflagellates as a Means of Detecting Toxicity in the Marine Environment

Lapota, David; Moskowitz, Gwendolyn J.; Rosenberger, Dena E.; Grovhoug, J G

doi:10.1520/stp13141s

Cited by 5 publications

(14 citation statements)

References 5 publications

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“…The photocyte bioassay was more than 30 times less sensitive to Cd and Cu than the bioluminescence bioassay using the dinoflagellate Pyrocystis lunula, yet the two bioassays showed similar sensitivity to Pb (Heimann et al, 2002). In contrast, the photocyte bioassay showed sensitivity to Cu and Zn similar to that of the bioluminescence bioassay when using the dinoflagellate Lingulodinium polyedrum (formerly Gonyaulax polyedra; Lapota et al, 1993). Therefore, the photocyte bioassay has a sensitivity range comparable to that of other bioluminescence-based bioassays using eukaryotic cells.…”

Section: Discussionmentioning

confidence: 92%

“…Metals are known to affect bioluminescence capacity in eukaryotic organisms (Okamoto et al, 1999;Deheyn et al, 2000b;Sudhaharan and Reddy, 2000), yet bioluminescence has been used as a proxy for metal toxicity only for dinoflagellates (Lapota et al, 1993;Heimann et al, 2002) and brittlestars (present study). The photocyte bioassay was more than 30 times less sensitive to Cd and Cu than the bioluminescence bioassay using the dinoflagellate Pyrocystis lunula, yet the two bioassays showed similar sensitivity to Pb (Heimann et al, 2002).…”

Section: Discussionmentioning

confidence: 95%

“…Most commonly used are luminescent bacteria and dinoflagellates, as well as genetically engineered bacteria that use luminescence reporters (Danilov et al, 1985;Lapota et al, 1993;Kudryasheva et al, 1998;Heimann et al, 2002). The assumption is that decreases in bioluminescence reflect the toxicity of a given condition, because light emission is a measure of the health and metabolism of the luminescent organism.…”

Section: Introductionmentioning

confidence: 99%

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Chemical speciation and toxicity of metals assessed by three bioluminescence‐based assays using marine organisms

Deheyn

Bencheikh-Latmani

Latz

2004

Environmental Toxicology

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ABSTRACT:Metal toxicity is a function of the biology of the target organism and the chemical speciation of the metal. The toxicity of 11 metals was assessed with three cell-based bioassays based on marine organisms: the bacterium Photobacterium phosphoreum of the Microtoxா bioassay, an environmental strain of P. phosphoreum, and photocytes isolated from the brittlestar Ophiopsila californica. Metal speciation was calculated for three commonly used media: NaCl-based Microtoxா bioassay medium, artificial seawater glycerol, and artificial seawater. Decreased bioluminescence was considered a proxy for cell toxicity. In all three assays the elements Cd and Hg exhibited similar speciation as well as similar toxicity profiles. The element Cu was toxic in all three assays despite different metal speciation for the P. phosphoreum bioassay. The element Ag was toxic to both bacterial strains but not to photocytes despite a similar chemical speciation for all three assays. In general, the Microtoxா bioassay was sensitive to all metals (except Pb), whereas the photocytes were the least sensitive to the metals. The heightened response of the Microtoxா bioassay probably resulted from a combination of the limited complexing power of the medium and the greater sensitivity of the bacterial strain.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chemical speciation and toxicity of metals assessed by three bioluminescence‐based assays using marine organisms

Deheyn

Bencheikh-Latmani

Latz

2004

Environmental Toxicology

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“…The most used dinoflagellate species in bioluminescence test are Ceratocorys horrida, Lingulodinium polyedrum (formerly Gonyaulax polyedra), Pyrocystis fusiformis, Pyrocystis lunula, Pyrocystis noctiluca, and Pyrophacus steinii. Lapota et al (1993) developed a toxicity bioassay based on these organisms to evaluate toxic effects of different substances (Lapota et al 1994). Commercial toxicity bioluminescence assays based on dinoflagellates include Lumitox ® (Lumitox Gulf L.C., USA) and QwikLite ® .…”

Section: Gerronema Viridilucensmentioning

confidence: 99%

Luminescent Microbial Bioassays and Microalgal Biosensors as Tools for Environmental Toxicity Evaluation

Hurtado-Gallego

Pulido-Reyes

González-Pleiter

et al. 2021

Handbook of Cell Biosensors

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This chapter deals with toxicity bioassays and biosensors based on luminescent microorganisms that report on global toxicity of a sample in such a way that luminescence is reduced or inhibited in the presence of toxic compounds that impair metabolism. Both natural and recombinant microorganisms are considered. A detailed description of their main characteristics and environmental applications is reported. Bioassays for detecting oxidative stress (both bioluminescent and fluorescent bioreporters) are also mentioned and discussed. Oxidative stress is caused by an unbalance between reactive oxygen species (ROS) and the Author contributed equally with all other contributors.

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“…Nonetheless, it is important to emphasize that bioassays that employ organisms of different trophic levels, should always be considered. In fact, the use of bioluminescent dinoflagellates as pollutants bioindicators can be considered a valuable tool, given the efficiency and low cost of carrying out such tests [ 21 , 22 , 23 , 24 , 25 , 26 , 27 ]. Furthermore, as they are eukaryotic cells, they can be considered better toxicity models for humans when compared to assays using bacteria [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Bioluminescent Dinoflagellates as a Bioassay for Toxicity Assessment

Perin

Moraes

Galeazzo

et al. 2022

IJMS

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Dinoflagellates bioluminescence mechanism depends upon a luciferin–luciferase reaction that promotes blue light emission (480 nm) in specialized luminogenic organelles called scintillons. The scintillons contain luciferin, luciferase and, in some cases, a luciferin-binding protein (LBP), which prevents luciferin from non-enzymatic oxidation in vivo. Even though dinoflagellate bioluminescence has been studied since the 1950s, there is still a lack of mechanistic understanding on whether the light emission process involves a peroxidic intermediate or not. Still, bioassays employing luminous dinoflagellates, usually from Gonyaulax or Pyrocystis genus, can be used to assess the toxicity of metals or organic compounds. In these dinoflagellates, the response to toxicity is observed as a change in luminescence, which is linked to cellular respiration. As a result, these changes can be used to calculate a percentage of light inhibition that correlates directly with toxicity. This current approach, which lies in between fast bacterial assays and more complex toxicity tests involving vertebrates and invertebrates, can provide a valuable tool for detecting certain pollutants, e.g., metals, in marine sediment and seawater. Thus, the present review focuses on how the dinoflagellates bioluminescence can be applied to evaluate the risks caused by contaminants in the marine environment.

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The Use of Stimulable Bioluminescence from Marine Dinoflagellates as a Means of Detecting Toxicity in the Marine Environment

Cited by 5 publications

References 5 publications

Chemical speciation and toxicity of metals assessed by three bioluminescence‐based assays using marine organisms

Chemical speciation and toxicity of metals assessed by three bioluminescence‐based assays using marine organisms

Luminescent Microbial Bioassays and Microalgal Biosensors as Tools for Environmental Toxicity Evaluation

Bioluminescent Dinoflagellates as a Bioassay for Toxicity Assessment

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