The use of ultra-high pressure liquid chromatography with tandem mass spectrometric detection in the analysis of agrochemical residues and mycotoxins in food – Challenges and applications

O’Mahony, John; Clarke, Lesa; Whelan, Michelle; O’Kennedy, Richard; Lehotay, Steven J.; Danaher, Martin

doi:10.1016/j.chroma.2013.01.007

Cited by 50 publications

(28 citation statements)

References 97 publications

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“…Operation in multiple-reaction monitoring (MRM) mode permits the measurement of multiple analytes (typically more than 150) in a single analytical run and thus it is not surprising that LC-MS/MS has become the method of choice for routine pesticide multi-residue analysis [1,2]. This trend is also observed in the field of veterinary drugs [3][4][5], mycotoxins [6][7][8], and in food safety and quality analysis in general [9,10]. Similar developments have taken place in other analytical domains including environmental analysis [11], forensic and clinical toxicology [12,13], and in human sports doping [14,15].…”

Section: Introductionmentioning

confidence: 93%

Identification in residue analysis based on liquid chromatography with tandem mass spectrometry: Experimental evidence to update performance criteria

Mol

Zomer

López

et al. 2015

Analytica Chimica Acta

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Section: Introductionmentioning

confidence: 93%

Identification in residue analysis based on liquid chromatography with tandem mass spectrometry: Experimental evidence to update performance criteria

Mol

Zomer

López

et al. 2015

Analytica Chimica Acta

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“…However, the most popular technique is high performance liquid chromatography (HPLC) with UV/Vis, fluorescence (FL) [17][18][19], or mass spectrometry (MS) detection [20][21][22]. Recently, ultrahigh performance liquid chromatography (UHPLC) coupled with tandem mass spectrometry (MS/MS) has become very popular, especially for multiclass determination of mycotoxins and for multiresidue determination with other contaminants [23][24][25][26]. Because of the complexity of food matrices, an extraction and clean-up purification step is usually required before analysis.…”

Section: Current Analytical Methods For Determination Of Mycotoxins Imentioning

confidence: 99%

Mycotoxin Analysis: New Proposals for Sample Treatment

Arroyo‐Manzanares

Huertas-Pérez

Garcı́a-Campaña

et al. 2014

Advances in Chemistry

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Mycotoxins are toxic secondary metabolites produced by different fungi, with different chemical structures. Mycotoxins contaminate food, feed, or raw materials used in their production and cause diseases and disorders in humans and livestock. Because of their great variety of toxic effects and their extreme heat resistance, the presence of mycotoxins in food and feed is considered a high risk to human and animal health. In order to ensure food quality and health consumers, European legislation has set maximum contents of some mycotoxins in different matrices. However, there are still some food commodities susceptible to fungal contamination, which were not contemplated in this legislation. In this context, we have developed new analytical techniques for the multiclass determination of mycotoxins in a great variety of food commodities (some of them scarcely studied), such as cereals, pseudocereals, cereal syrups, nuts, edible seeds, and botanicals. Considering the latest technical developments, ultrahigh performance liquid chromatography coupled to tandem mass spectrometry has been chosen as an efficient, fast, and selective powerful analytical technique. In addition, alternative sample treatments based on emerging methodologies, such as dispersive liquid-liquid microextraction and QuEChERS, have been developed, which allow an increased efficiency and sample throughput, as well as reducing contaminant waste.

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“…Several validated methods are available for the measurement of mycotoxins and the literature on this subject has included many comprehensive and critical reviews [8,[15][16][17][18][19]. For the quantitative analysis of mycotoxins high performance liquid chromatography (HPLC), gas chromatography (GC) and thin layer chromatography (TLC) as well as multiple toxin mass spectrometry (MS) methods are used In addition to safety issues, natural toxin contamination of food is of great economic concern; much effort is therefore devoted to the development of novel, rapid, inexpensive, simple and sensitive multiple mycotoxin screening methods.…”

Section: General Rightsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a nanoarray capable of the rapid and simultaneous detection of zearalenone, T2-toxin and fumonisin

McNamee

Bravin

Rosar

et al. 2017

Talanta

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K. (2017). Development of a nanoarray capable of the rapid and simultaneous detection of zearalenone, T2-toxin and fumonisin. Talanta, 164, 368-376. DOI: 10.1016/j.talanta.2016 Published in: Talanta Document Version: Peer reviewed version Queen's University Belfast -Research Portal: Link to publication record in Queen's University Belfast Research Portal Publisher rights © 2017 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/bync-nd/4.0/ which permits distribution and reproduction for non-commercial purposes, provided the author and source are cited. General rightsCopyright for the publications made accessible via the Queen's University Belfast Research Portal is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights.Take down policy The Research Portal is Queen's institutional repository that provides access to Queen's research output. Every effort has been made to ensure that content in the Research Portal does not infringe any person's rights, or applicable UK laws. If you discover content in the Research Portal that you believe breaches copyright or violates any law, please contact openaccess@qub.ac.uk. Fusarium mycotoxins such as trichothecenes, zearalenone and fumonisins occur on a worldwide basis in cereal grains, animal feeds and forages. Practical solutions for multiple mycotoxin determination in samples are required by industry and regulators for cost effective screening purposes. The feasibility of developing a novel multiplex nanoarray for the simultaneous and semi-quantitative detection of three regulated mycotoxins: zearalenone (ZEA), T2-toxin (T2) and fumonisin B1 (FUM) was examined. Additionally, the assay was also able to detect HT2 toxin and fumonisin B2 and B3 due to the cross reactivity profiles of the antibodies used. Individual mycotoxin conjugates specific to the three mycotoxins were nano-spotted onto wells of a microtitre plate. Optimisation of assay parameters and antibodies was undertaken with both individual and multiplex calibration curves generated. A competitive assay format was employed enabling a calibration curve for concentration analysis and duplicate results for up to 40 samples in 70 min for the three target mycotoxins. The characteristics and performance of the nanoarray were evaluated including sensitivity and specificity for each target. Additionally, intra and inter spotting precision, cross reactivity, matrix effects and sample analysis in maize and wheat (n=8) was performed. Sensitivity, determined as the concentration causing 50 % inhibition, was 70.1, 2.8 and 90.9 ppb in PBS, 172.4, 3.2 and 129.3 ppb in methanol, 197.4, 0.7 and 216.7 ppb in wheat and 43.6, 0.5 and 25.9 ppb in maize for ZEA, T2 and FUM respectively. Intra spotting precision was 6, 11 and 10 % for PBS and 5, 11 and 12 % for methanol for ZEA, T2 and FUM respectively. Inter spotti...

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The use of ultra-high pressure liquid chromatography with tandem mass spectrometric detection in the analysis of agrochemical residues and mycotoxins in food – Challenges and applications

Cited by 50 publications

References 97 publications

Identification in residue analysis based on liquid chromatography with tandem mass spectrometry: Experimental evidence to update performance criteria

Identification in residue analysis based on liquid chromatography with tandem mass spectrometry: Experimental evidence to update performance criteria

Mycotoxin Analysis: New Proposals for Sample Treatment

Development of a nanoarray capable of the rapid and simultaneous detection of zearalenone, T2-toxin and fumonisin

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