The Use of Ultraviolet Resonance Raman Spectroscopy in the Analysis of Ionizing-Radiation-Induced Damage in DNA

Shaw, Conor; Jirásek, A

doi:10.1366/000370209787944325

Cited by 12 publications

(11 citation statements)

References 31 publications

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“…Spectroscopic techniques are powerful tools for detecting and studying DNA lesions. Their chemical sensitivity makes them the most accurate tool for the detection of even small structural changes [32]. Moreover, techniques based on the interaction of electromagnetic field with chemical bonds enables us to receive the fingerprint spectra of the investigated molecules, allowing for the simultaneous detection of various functional groups typical for the investigated sample.…”

Section: Introductionmentioning

confidence: 99%

Molecular Spectroscopic Markers of DNA Damage

Sofińska¹,

Wilkosz²,

Szymoński³

et al. 2020

Molecules

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Every cell in a living organism is constantly exposed to physical and chemical factors which damage the molecular structure of proteins, lipids, and nucleic acids. Cellular DNA lesions are the most dangerous because the genetic information, critical for the identity and function of each eukaryotic cell, is stored in the DNA. In this review, we describe spectroscopic markers of DNA damage, which can be detected by infrared, Raman, surface-enhanced Raman, and tip-enhanced Raman spectroscopies, using data acquired from DNA solutions and mammalian cells. Various physical and chemical DNA damaging factors are taken into consideration, including ionizing and non-ionizing radiation, chemicals, and chemotherapeutic compounds. All major spectral markers of DNA damage are presented in several tables, to give the reader a possibility of fast identification of the spectral signature related to a particular type of DNA damage.

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Section: Introductionmentioning

confidence: 99%

Molecular Spectroscopic Markers of DNA Damage

Sofińska¹,

Wilkosz²,

Szymoński³

et al. 2020

Molecules

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“…Other studies have applied RS for cellular biochemical analysis of apoptosis (Verrier et al 2004, Zoladek et al 2011, necrosis (Kunapareddy et al 2008), cell death (Notingher et al 2003), non-proliferation (Short et al 2005) and cell cycle (Swain et al 2008, Matthews et al 2010. In radiobiological applications, RS has detected molecular alterations in irradiated aqueous DNA (Sailer et al 1996, Shaw andJirasek 2009), sodium hyaluronate (Synytsya et al 2011), biological membranes (Verma 1986, Verma and Rastogi 1990, Verma and Sonwalkar 1991, Verma et al 1993, Sailer et al 1997, and skin and muscle tissues (Lakshmi et al 2002, Synytsya et al 2004. A recent clinical RS study discriminated between responding and non-responding cervical cancers post-irradiation (Vidyasagar et al 2008), yet made no conclusions regarding the differences in the biochemical composition between tissues or the molecular basis for RS discrimination.…”

Section: Introductionmentioning

confidence: 99%

Biochemical signatures ofin vitroradiation response in human lung, breast and prostate tumour cells observed with Raman spectroscopy

et al. 2011

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This work applies noninvasive single-cell Raman spectroscopy (RS) and principal component analysis (PCA) to analyze and correlate radiation-induced biochemical changes in a panel of human tumour cell lines that vary by tissue of origin, p53 status and intrinsic radiosensitivity. Six human tumour cell lines, derived from prostate (DU145, PC3 and LNCaP), breast (MDA-MB-231 and MCF7) and lung (H460), were irradiated in vitro with single fractions (15, 30 or 50 Gy) of 6 MV photons. Remaining live cells were harvested for RS analysis at 0, 24, 48 and 72 h post-irradiation, along with unirradiated controls. Single-cell Raman spectra were acquired from 20 cells per sample utilizing a 785 nm excitation laser. All spectra (200 per cell line) were individually post-processed using established methods and the total data set for each cell line was analyzed with PCA using standard algorithms. One radiation-induced PCA component was detected for each cell line by identification of statistically significant changes in the PCA score distributions for irradiated samples, as compared to unirradiated samples, in the first 24-72 h post-irradiation. These RS response signatures arise from radiation-induced changes in cellular concentrations of aromatic amino acids, conformational protein structures and certain nucleic acid and lipid functional groups. Correlation analysis between the radiation-induced PCA components separates the cell lines into three distinct RS response categories: R1 (H460 and MCF7), R2 (MDA-MB-231 and PC3) and R3 (DU145 and LNCaP). These RS categories partially segregate according to radiosensitivity, as the R1 and R2 cell lines are radioresistant (SF(2) > 0.6) and the R3 cell lines are radiosensitive (SF(2) < 0.5). The R1 and R2 cell lines further segregate according to p53 gene status, corroborated by cell cycle analysis post-irradiation. Potential radiation-induced biochemical response mechanisms underlying our RS observations are proposed, such as (1) the regulated synthesis and degradation of structured proteins and (2) the expression of anti-apoptosis factors or other survival signals. This study demonstrates the utility of RS for noninvasive radiobiological analysis of tumour cell radiation response, and indicates the potential for future RS studies designed to investigate, monitor or predict radiation response.

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“…It was shown that a DUV resonant Raman spectrum varies with microenvironment of nucleotide bases in a nucleic acid . The spectrum was used for analyzing nucleic acid conformation, such as supercoiling, folding, and melting …”

Section: Resonant Raman Scattering For Imaging and Analysismentioning

confidence: 99%

Deep‐Ultraviolet Biomolecular Imaging and Analysis

Kumamoto

Taguchi

Kawata

2018

Advanced Optical Materials

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Deep‐ultraviolet light, 200–300 nm in wavelength, interacts with nucleic acids and proteins strongly compared to visible and infrared light. In this article, the interaction between deep‐ultraviolet photons and biomolecules is discussed. Especially, the absorption and autofluorescence of biomolecules by the deep‐ultraviolet excitation are examined. Applications of deep‐ultraviolet absorption and autofluorescence to label‐free biomolecular imaging and analysis of cells and tissues are shown. Resonant Raman scattering at nucleotide bases and aromatic amino acids as another important topic of deep‐ultraviolet photonics is reviewed in biomolecular imaging and analysis. The discussion extends to the recent progress in the development of deep‐ultraviolet microscopes as well as a variety of deep‐ultraviolet optical devices such as light sources, detectors, and objectives. The issue of photodamage due to deep‐ultraviolet irradiation on cells and tissues is also discussed. It has been recently suppressed with use of lanthanide ions. The experimental results of the photodamage suppression are shown. For surface‐enhanced resonant Raman scattering, autofluorescence enhancement, and tip‐enhanced resonant Raman scattering microscopy, plasmonic materials applicable in the deep‐ultraviolet range are discussed.

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The Use of Ultraviolet Resonance Raman Spectroscopy in the Analysis of Ionizing-Radiation-Induced Damage in DNA

Cited by 12 publications

References 31 publications

Molecular Spectroscopic Markers of DNA Damage

Molecular Spectroscopic Markers of DNA Damage

Biochemical signatures ofin vitroradiation response in human lung, breast and prostate tumour cells observed with Raman spectroscopy

Deep‐Ultraviolet Biomolecular Imaging and Analysis

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