2018
DOI: 10.3390/fermentation4010018
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The Use of UV-Vis Spectroscopy in Bioprocess and Fermentation Monitoring

Abstract: Real-time analytical tools to monitor bioprocess and fermentation in biological and food applications are becoming increasingly important. Traditional laboratory-based analyses need to be adapted to comply with new safety and environmental guidelines and reduce costs. Many methods for bioprocess fermentation monitoring are spectroscopy-based and include visible (Vis), infrared (IR) and Raman. This paper describes the main principles and recent developments in UV-Vis spectroscopy to monitor bioprocess and ferme… Show more

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Cited by 35 publications
(6 citation statements)
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References 53 publications
(254 reference statements)
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“…The UV-Visible spectrophotometer (Thermoscientific Genesys 180) used to prepare the calibration curve of drug to show the relation between concentration and absorbance to figure out the drug content and drug release study. The stock solution (1 mg/mL) was prepared in dimethyl sulfoxide (DMSO) and further dilutions (20,40,60,80,100, 120 µg/mL) were prepared and scanned at maximum absorbance (λmax) ( (Roberts et al, 2018;Rombaut et al, 2015).…”
Section: Fourier-transform Infrared Spectroscopy (Ftir)mentioning
confidence: 99%
“…The UV-Visible spectrophotometer (Thermoscientific Genesys 180) used to prepare the calibration curve of drug to show the relation between concentration and absorbance to figure out the drug content and drug release study. The stock solution (1 mg/mL) was prepared in dimethyl sulfoxide (DMSO) and further dilutions (20,40,60,80,100, 120 µg/mL) were prepared and scanned at maximum absorbance (λmax) ( (Roberts et al, 2018;Rombaut et al, 2015).…”
Section: Fourier-transform Infrared Spectroscopy (Ftir)mentioning
confidence: 99%
“…The spectra of materials can be measured in the wavelength range of 800 nm to 2500 nm using a ultraviolet spectrophotometer (UV), visible spectrophotometer (vis), and near-infrared spectrophotometer (NIR). 99,145,146 Composite quantification with these instruments uses ultraviolet and visible light in the wavelength range of 200 and 780 nm. 99 Studies show that these instruments induce analyte electronic transitions such as π → π*, n → π*, n → σ*, δ → δ and charge transfer transitions.…”
Section: Selected Instrumental Characterization Of Fabricated Pani Co...mentioning
confidence: 99%
“…Ewanick et al [49,50] UV-Vis spectroscopy is often not considered as a method for real-time monitoring of fermentations because it cannot detect many key compounds (e.g. glucose or ethanol) and because the light scattering caused by the suspended solid particles dominates the absorption process [35,52]. However, in the context of cellulose to ethanol fermentation, the technique gains special relevance because many of the inhibitors present in lignocellulosic hydrolysate including furfural, HMF or acetic acid absorb in this region [53].…”
Section: Vibrational Spectroscopymentioning
confidence: 99%