The Use of Water-Soluble and Basic Carbodiimides in Peptide Synthesis

Aqueous Microwave-Assisted Solid-Phase Synthesis Using Boc-Amino Acid Nanoparticles

“…We also successfully prepared water-dispersible Boc-Tyr(BrZ)-OH, which has a hydrophobic BrZ side chain protecting group [11]. WSCI [31], DMTMM [32,33] and combinations of an additive sulfo-HOSu [34] were examined [24]. Reaction temperature was 70 °C using a MW power of 70 W (Figure 1).…”

Decellularization, Stabilization and Functionalization of Collagenous Tissues Used as Cardiovascular Biomaterials

“…The crosslinking agents used in collagen-rich biomaterials can use both primary amino groups and acid groups of polypeptide chains. Historically, a water soluble carbodiimide (1-ethyl-3-(3-dimethyl amino propyl) carbodiimide / EDAC) was first used for the modification of carboxylic groups in proteins for peptide synthesis (Sheehan & Hlavka, 1956) and to promote crosslinking in gelatin (Sheehan & Hlavka, 1957). The mechanism for the reaction between carboxylic groups and EDAC leading to amide bond formation is as follows: The addition of a carboxylic acid diimide produces an isourea ester, an O-acyl isourea.…”

The use of graft copolymers as enzyme supports immobilization of proteins and enzymes on a hydrolyzed nylon‐co‐acrylonitrile system

“…The condensing agent used was I-cyclohexyl-3 (2-morpholinoethyl)-carbodiimide metho-p-toluene sulphonate (CMC). 4 Line et al? used CMC to immobilize pepsin onto amino-alkylated porous glass, and similarly Wondolowski and Woychick' immobilized p-galactosidase, although in both instances the activities of the resulting polymers were very low.…”