The Use of Zein and Shuanghuangbu for Periodontal Tissue Engineering

Abstract

Aim Tissue engineering is a promising area with a broad range of applications in the fields of regenerative medicine and human health. The emergence of periodontal tissue engineering for clinical treatment of periodontal disease has opened a new therapeutic avenue. The choice of scaffold is crucial. This study was conducted to prepare zein scaffold and explore the suitability of zein and Shuanghuangbu for periodontal tissue engineering. Methodology A zein scaffold was made using the solvent casting/particulate leaching method with sodium chloride (NaCl) particles as the porogen. The physical properties of the zein scaffold were evaluated by observing its shape and determining its pore structure and porosity. Cytotoxicity PDLCs. Altogether, these findings provide the basis for in vivo testing on animals.

Alimentary ‘green’ proteins as electrospun scaffolds for skin regenerative engineering

“…Wheat gluten was most likely the first plant protein to have been electrospun (Woerdeman et al ., ), paving the way for fabrication of nanofibrous or submicron fibrous scaffolds from other plant‐derived proteins. For example, corn zein has been shown to be a suitable biomaterial in the form of films (Sun et al ., ) and porous scaffolds (Gong et al ., ; Wang et al ., ; Tu et al ., ; Xu et al ., ), and has been electrospun by different groups (Yao et al ., ; Selling et al ., ) using a variety of solvents.…”

Stem cell culture media enriched with plant‐derived compounds: Cell differentiation enhancement

“…Dental pulp SCs (DPSCs) are isolated from tooth pulp and share a differential potential similar to MSCs as they are able to transdifferentiate into mesoderm (osteocytes, chondrocytes, adipocytes and smooth muscle cells) and ectoderm (neurocytes) lineages 62 . Due to the ease of obtaining them and their potential to differentiate into bone cell phenotype, DPSCs are currently studied to treat dental diseases like periodontal diseases consisting of a chronic infection that affects teeth function leading to their loss 63 . Therefore, the use of DPSCs to stimulate bone regeneration represents a promising alternative for treatment.…”

“…62 Due to the ease of obtaining them and their potential to differentiate into bone cell phenotype, DPSCs are currently studied to treat dental diseases like periodontal diseases consisting of a chronic infection that affects teeth function leading to their loss. 63 Therefore, the use of DPSCs to stimulate bone regeneration represents a promising alternative for treatment. However, the efficiency of DPSC therapy can be improved through the addition of phytochemicals promoting osteogenic differentiation.…”

Status of Plant Protein-Based Green Scaffolds for Regenerative Medicine Applications

“…In a similar study, a zein porous scaffold was produced by means of a solvent casting/particulate leaching process. The scaffold with good biocompatibility, proper porosity (64.1%–78.0%), and well interconnectivity was suitable for the development of periodontal ligament cells (PDLCs) [201]. In another study, Wu et al [202] created porous scaffolds of zein/PCL composites through a solvent casting/particulate leaching technique to regenerate bone.…”