The Use of Zwitterionic Methylmethacrylat Coated Silicone Inhibits Bacterial Adhesion and Biofilm Formation of Staphylococcus aureus

Woitschach, Franziska; Kloss, M.; Schlodder, Karsten; Rabes, Anne; Mörke, Caroline; Oschatz, Stefan; Senz, Volkmar; Borck, Alexander; Grabow, Niels; Reisinger, Emil C.; Sombetzki, Martina

doi:10.3389/fbioe.2021.686192

Cited by 9 publications

(9 citation statements)

References 59 publications

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“…The in vivo model presented in this study can be extended to any implantation duration to additionally investigate drug-resistant behavior of the bacteria against the tested material. Antimicrobial material grafting or functionalization implement their antimicrobial function via immobilization of antimicrobial peptides [29] or physicochemical changes such as polycationic [30] or zwitterionic coatings [31] that may interrupt the net negative charge of the bacteria membrane. But under in vivo conditions in the bloodstream, various other physical, chemical and biological conditions may act on these materials that might affect the physicochemical properties and therefore impact the life-span of the antimicrobial properties.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a Murine Model for Testing Antimicrobial Implant Materials in the Blood Circulation System

et al. 2021

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Medical device-related infections are becoming a steadily increasing challenge for the health care system regarding the difficulties in the clinical treatment. In particular, cardiovascular implant infections, catheter-related infections, as well as infective endocarditis are associated with high morbidity and mortality risks for the patients. Antimicrobial materials may help to prevent medical device-associated infections and supplement the currently available therapies. In this study, we present an easy-to-handle and simplified in vivo model to test antimicrobial materials in the bloodstream of mice. The model system is composed of the implantation of a bacteria-laden micro-stent scaffold into the murine tail vein. Our model enables the simulation of catheter-related infections as well as the development of infective endocarditis specific pathologies in combination with material testing. Furthermore, this in vivo model can cover two phases of the biofilm formation, including both the local tissue response to the bacterial biofilm and the systemic inflammatory response against circulating bacteria in the bloodstream that detached from a mature biofilm.

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Section: Discussionmentioning

confidence: 99%

Evaluation of a Murine Model for Testing Antimicrobial Implant Materials in the Blood Circulation System

et al. 2021

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“…Two thermoplastic polyurethanes (TPU) and liquid silicone rubber (LSR) were used, either with or without modification. Further details on the preparation are described in our previous work ( Woitschach et al., 2021a ; Woitschach et al., 2021b ). After modification, all samples were subjected to a sterilization process with ethylene oxide (ETO).…”

Section: Methodsmentioning

confidence: 99%

“…In our previous work, we investigated the biofilm formation ability of two different staphylococcal strains on liquid silicone rubber (LSR) surfaces modified with PMMA-MPC, PSU, and PPSP compared to two of the most commonly used thermoplastic polyurethanes (TPU). We found an inhibitory effect for Staphylococcus aureus (ATCC35556) on LSR coated with PMMA-MPC ( Woitschach et al., 2021a ). In this study, we would like to extend our protocol for analyzing bacterial colonization and biofilm formation on these materials by Enterococcus faecalis .…”

Section: Introductionmentioning

confidence: 91%

Bacterial Adhesion and Biofilm Formation of Enterococcus faecalis on Zwitterionic Methylmethacrylat and Polysulfones

Woitschach

Kloss

Schlodder

et al. 2022

Front. Cell. Infect. Microbiol.

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Biofilm-associated implant infections represent a major challenge for healthcare systems around the world due to high patient burden and enormous costs incurred. Enterococcus faecalis (E. faecalis) is the most prevalent enterococcal species identified in biofilm-associated infections. The steadily growing areas of application of implants demand a solution for the control of bacterial infections. Therefore, the development of modified anti-microbial implant materials and the testing of the behavior of different relevant bacterial strains towards them display an indispensable task. Recently, we demonstrated an anti-microbial effect of zwitterionic modified silicone rubber (LSR) against Staphylococcus aureus. The aim of this study was to evaluate bacterial colonization and biofilm formation of another clinically relevant strain, E. faecalis, on this material in comparison to two of the most commonly used thermoplastic polyurethanes (TPUs) and other modified LSR surfaces. By generating growth curves, crystal violet, and fluorescence staining, as well as analyzing the expression of biofilm-associated genes, we demonstrated no anti-microbial activity of the investigated materials against E. faecalis. These results point to the fact that anti-microbial effects of novel implant materials do not always apply across the board to all bacterial strains.

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“…We were able to show another positive effect. In our previous work, we could show that PMMA-MPC has a significant anti-biofilm formation effect on S. aureus [20].…”

Section: Introductionmentioning

confidence: 92%

“…Endotoxin levels for all materials were beneath 0.2 EU/mL. For further specifications of all investigated materials, see Woitschach et al [20]. Discoidal samples of thermanox (TMX), a well-characterized polyester, were used as reference material.…”

Section: Polymer Functionalizationmentioning

confidence: 99%

In Vitro Study of the Interaction of Innate Immune Cells with Liquid Silicone Rubber Coated with Zwitterionic Methyl Methacrylate and Thermoplastic Polyurethanes

et al. 2021

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The biocompatibility of medical devices, such as implants and prostheses, is strongly determined by the host’s immune response to the implanted material. Monocytes and macrophages are main actors of the so-called foreign body reaction. The innate immune system macrophages (M) can be broadly classified into the pro-inflammatory M1-type and the anti-inflammatory, pro-healing M2-type. While a transient inflammatory initial state can be helpful during an infection, persistent inflammation interferes with proper healing and subsequent regeneration. The functional orientation of the immune response, mirrored by monocyte polarization, during interaction with different biomaterials has not yet been sufficiently explored. In implant manufacturing, thermoplastic polyurethane (TPU) represents the state-of-the-art material. The constantly growing areas of application and the associated necessary adaptations make the optimization of these materials indispensable. In the present study, modified liquid silicone rubber (LSR) were compared with two of the most commonly used TPUs, in terms of monocyte adhesion and M1/M2 polarization in vitro. Human monocytes isolated from venous blood were evaluated for their ability to adhere to various biomaterials, their gene expression profile, and their cytokine release. Based on the results, the different polymers exhibit different potential to bias monocytes with respect to early pro-inflammatory cytokine production and gene transcription. Furthermore, none of our test materials showed a clear trend towards M1 or M2 polarization. However, we were able to evaluate the inflammatory potential of the materials, with the classic TPUs appearing to be the most unreactive compared to the silicone-based materials.

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The Use of Zwitterionic Methylmethacrylat Coated Silicone Inhibits Bacterial Adhesion and Biofilm Formation of Staphylococcus aureus

Cited by 9 publications

References 59 publications

Evaluation of a Murine Model for Testing Antimicrobial Implant Materials in the Blood Circulation System

Evaluation of a Murine Model for Testing Antimicrobial Implant Materials in the Blood Circulation System

Bacterial Adhesion and Biofilm Formation of Enterococcus faecalis on Zwitterionic Methylmethacrylat and Polysulfones

In Vitro Study of the Interaction of Innate Immune Cells with Liquid Silicone Rubber Coated with Zwitterionic Methyl Methacrylate and Thermoplastic Polyurethanes

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