The Usefulness of a Duplex RT-qPCR during the Recent Yellow Fever Brazilian Epidemic: Surveillance of Vaccine Adverse Events, Epizootics and Vectors

Queiroz, Alice Louize Nunes; Barros, Rafael S; Silva, Sandro P.; Rodrigues, Daniela Sueli Guerreiro; Cruz, Ana Cecília Ribeiro; Santos, Flávia Barreto dos; Vasconcelos, Pedro F. C.; Tesh, Robert B.; Nunes, Bruno Tardelli Diniz; Medeiros, Daniele B. A.

doi:10.3390/pathogens10060693

Cited by 6 publications

(2 citation statements)

References 59 publications

(83 reference statements)

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“…The YFV molecular sequence obtained was closer to West African sequences ( Fig. 5 ), than to American sequences previously reported [ 42 ]. This phenomenon could be associated with the continuous introduction of African YFV into the America [ 43 ].…”

Section: Discussionsupporting

confidence: 63%

Multidisciplinary approach for surveillance and risk identification of yellow fever and other arboviruses in Colombia

et al. 2022

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Section: Discussionsupporting

confidence: 63%

Multidisciplinary approach for surveillance and risk identification of yellow fever and other arboviruses in Colombia

et al. 2022

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“…Data regarding PCR sensitivities are scarce. A Brazilian study conducted after the last outbreak from 2016 to 2018 indicated detection rates of 8.9% in serum and 50% in blood samples during the viremic phase [98]. Urine sample sensitivity was evaluated as 25% [99].…”

Section: Diagnosismentioning

confidence: 99%

Ocular Manifestations of Flavivirus Infections

Zina,

Hoarau,

Labetoulle

et al. 2023

Pathogens

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Flaviviruses are a group of positive-sense, single-stranded RNA viruses predominantly transmitted by arthropods (mainly mosquitoes) that cause severe endemic infections and epidemics on a global scale. They represent a major cause of systemic morbidity and death and are expanding worldwide. Among this group, dengue fever, the West Nile virus, yellow fever, Japanese Encephalitis, and, recently, the Zika virus have been linked to a spectrum of ocular manifestations. These manifestations encompass subconjunctival hemorrhages and conjunctivitis, anterior and posterior uveitis (inclusive of vitritis, chorioretinitis, and retinal vasculitis), maculopathy, retinal hemorrhages, and optic neuritis. Clinical diagnosis of these infectious diseases is primarily based on epidemiological data, history, systemic symptoms and signs, and the pattern of ocular involvement. Diagnosis confirmation relies on laboratory testing, including RT-PCR and serological testing. Ocular involvement typically follows a self-limited course but can result in irreversible visual impairment. Effective treatments of flavivirus infections are currently unavailable. Prevention remains the mainstay for arthropod vector and zoonotic disease control. Effective vaccines are available only for the yellow fever virus, dengue virus, and Japanese Encephalitis virus. This review comprehensively summarizes the current knowledge regarding the ophthalmic manifestations of the foremost flavivirus-associated human diseases.

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17DD-yellow-fever-vaccine

2021

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17DD-yellow-fever-vaccine Various toxicities: 2 case reportsIn a study of 872 patients conducted between 2016 and 2018, a 30-year-old man and a 67-year-old woman were described, who developed viscerotropic disease, haematemesis, melena, epistaxis, gum bleeding, abdominal pain, Faget signs and renal excretion disturbance following vaccination with 17DD-yellow-fever-vaccine in Brazil.The patients were vaccinated with 17DD-yellow-fever-vaccine [dosages and routes not stated]. Subsequently, they were found positive for yellow fever virus WT genome using the duplex RT-qPCR 17DD/African strain primer/probe. The findings suggested 17DD-yellow-fever-vaccine associated viscerotropic disease. Three and two days following the vaccination, the patient developed signs and symptoms that were similar to a classic haemorrhagic yellow fever comprising haematemesis, melena, epistaxis, gum bleeding, abdominal pain, Faget signs (high temperature with slow pulse rate) and renal excretion disturbances secondary to the vaccination. Both the patients recovered despite the symptoms.Queiroz ALN, et al. The usefulness of a duplex rt-qpcr during the recent yellow fever brazilian epidemic: Surveillance of vaccine adverse events, epizootics and vectors.

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The Usefulness of a Duplex RT-qPCR during the Recent Yellow Fever Brazilian Epidemic: Surveillance of Vaccine Adverse Events, Epizootics and Vectors

Cited by 6 publications

References 59 publications

Multidisciplinary approach for surveillance and risk identification of yellow fever and other arboviruses in Colombia

Multidisciplinary approach for surveillance and risk identification of yellow fever and other arboviruses in Colombia

Ocular Manifestations of Flavivirus Infections

17DD-yellow-fever-vaccine

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