The utility of flow cytometric platelet forward scatter as an alternative to mean platelet volume

Connor, David; Rabbolini, David J.; Fixter, Kate; Donikian, Dea; Kondo, Mayuku; Chan, Onki; Jarvis, Suzanne; Chen, Walter; Brighton, Timothy; Chen, Vivien; Ward, Christopher; Joseph, Joanne

doi:10.1080/09537104.2022.2052035

Cited by 4 publications

(2 citation statements)

References 20 publications

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“…This result is similar to those obtained through absolute platelet counts carried out at the site of sample collection ( Table 3 ). We then compared the mean platelet volume as estimated by the forward scatter analysis using flow cytometry ( 34 ). Figure 1C shows a distribution and rug plot comparing subject counts and FSC mean value distributions.…”

Section: Resultsmentioning

confidence: 99%

Platelets’ morphology, metabolic profile, exocytosis, and heterotypic aggregation with leukocytes in relation to severity and mortality of COVID-19-patients

Yasseen

Elkhodiry²,

El-Messiery³

et al. 2022

Front. Immunol.

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Roles of platelets during infections surpass the classical thrombus function and are now known to modulate innate immune cells. Leukocyte-platelet aggregations and activation-induced secretome are among factors recently gaining interest but little is known about their interplay with severity and mortality during the course of SARS-Cov-2 infection. The aim of the present work is to follow platelets’ bioenergetics, redox balance, and calcium homeostasis as regulators of leukocyte-platelet interactions in a cohort of COVID-19 patients with variable clinical severity and mortality outcomes. We investigated COVID-19 infection-related changes in platelet counts, activation, morphology (by flow cytometry and electron microscopy), bioenergetics (by Seahorse analyzer), mitochondria function (by high resolution respirometry), intracellular calcium (by flow cytometry), reactive oxygen species (ROS, by flow cytometry), and leukocyte-platelet aggregates (by flow cytometry) in non-intensive care unit (ICU) hospitalized COVID-19 patients (Non-ICU, n=15), ICU-survivors of severe COVID-19 (ICU-S, n=35), non-survivors of severe COVID-19 (ICU-NS, n=60) relative to control subjects (n=31). Additionally, molecular studies were carried out to follow gene and protein expressions of mitochondrial electron transport chain complexes (ETC) in representative samples of isolated platelets from the studied groups. Our results revealed that COVID-19 infection leads to global metabolic depression especially in severe patients despite the lack of significant impacts on levels of mitochondrial ETC genes and proteins. We also report that severe patients’ platelets exhibit hyperpolarized mitochondria and significantly lowered intracellular calcium, concomitantly with increased aggregations with neutrophil. These changes were associated with increased populations of giant platelets and morphological transformations usually correlated with platelets activation and inflammatory signatures, but with impaired exocytosis. Our data suggest that hyperactive platelets with impaired exocytosis may be integral parts in the pathophysiology dictating severity and mortality in COVID-19 patients.

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Section: Resultsmentioning

confidence: 99%

Platelets’ morphology, metabolic profile, exocytosis, and heterotypic aggregation with leukocytes in relation to severity and mortality of COVID-19-patients

Yasseen

Elkhodiry²,

El-Messiery³

et al. 2022

Front. Immunol.

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“…Variants associated with DIAPH1-related macrothrombocytopenia result in the constant activation of the DIAPH1 protein, leading to disruption of cytoskeletal and microtubule function, thereby impairing proplatelet formation [ 1 ]. Patients with rare causative variants in DIAPH1 have moderate macrothrombocytopenia without bleeding complications, but often mild neutropenia and severe sensorineural deafness can also be observed [ 8 ]. Our pediatric patient exhibited moderate thrombocytopenia without associated bleeding complications, along with hearing loss necessitating cochlear transplantation.…”

Section: Discussionmentioning

confidence: 99%

A Novel Variant in the DIAPH1 Gene Causing Macrothrombocytopenia and Non-syndromic Hearing Loss in a Pediatric Saudi Girl

Alasmari,

Alpakra,

Hassanien

et al. 2024

Cureus

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Macrothrompocytopenia (MTP) is a rare group of hereditary disorders that lead to impaired hemostasis. Macrothrompocytopenia mostly results from genetic mutations in genes implicated in megakaryocyte differentiation and function. Diaphanous-related formin 1 (DIAPH1) is a protein-coding gene. Dominant gain-of-function DIAPH1 variants cause macrothrombocytopenia and sensorineural deafness (autosomal dominant non-syndromic hearing loss 1 (DFNA1)), while homozygous loss of DIAPH1 results in seizures, cortical blindness, and microcephaly syndrome (SCBMS). This rare genetic disease is characterized by progressive and severe hearing loss with onset in the first decade of life, is associated with mild thrombocytopenia, and has no significant bleeding tendency. This case report presents the clinical findings of a 14-year-old Saudi pediatric girl. We investigated the potential association of DIAPH1 as a novel candidate gene linked to dominant MTP and autosomal dominant non-syndromic hearing loss (ADNSHL), which was evaluated through audiometry. Notably, a novel variant, c.3633_3636del, was identified in the DIAPH1 gene. To date, only a small number of mutations in this gene have been reported as the cause of MTP and ADNSHL.

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Laboratory Markers of Platelet Production and Turnover

Бодрова

Shustova

Хаспекова

et al. 2023

Biochemistry Moscow

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The utility of flow cytometric platelet forward scatter as an alternative to mean platelet volume

Cited by 4 publications

References 20 publications

Platelets’ morphology, metabolic profile, exocytosis, and heterotypic aggregation with leukocytes in relation to severity and mortality of COVID-19-patients

Platelets’ morphology, metabolic profile, exocytosis, and heterotypic aggregation with leukocytes in relation to severity and mortality of COVID-19-patients

A Novel Variant in the DIAPH1 Gene Causing Macrothrombocytopenia and Non-syndromic Hearing Loss in a Pediatric Saudi Girl

Laboratory Markers of Platelet Production and Turnover

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