The utility of functional interaction and cluster analysis in CNS proteomics

Deighton, Ruth F.; Short, Duncan M.; McGregor, Richard; Gow, Alan J.; Whittle, Ian R.; McCulloch, James

doi:10.1016/j.jneumeth.2009.03.013

Cited by 9 publications

(15 citation statements)

References 28 publications

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“…Changes in HSPB6 expression have been found in several other cancers. In gliomas, HSPB6 was reproducibly downregulated in tissue specimens when compared to normal brain tissues [88]; in primary lung cell adenocarcinomas HSPB6 was substantially downregulated [89]. Mean anti-HSPB6 antibody concentrations were negatively correlated with ovarian cancer malignancy.…”

Section: Role Of Hspb6 In Cancermentioning

confidence: 96%

Study of HSPB6: Insights into the Properties of the Multifunctional Protective Agent

Xiao

Zhou

et al. 2017

Cell Physiol Biochem

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HSPB6(Heat shock protein B6), is also referred to as P20/HSP20. Unlike other many other members of sHSP(small Heat shock protein) family, which tend to form high-molecular-mass oligomers, in solution, human HSPB6 only forms dimers. However, it still exhibits chaperon-like activity comparable with that of HSPB5. It is expressed ubiquitously, with high and constitutive expression in muscular tissues. sHSPs characteristically function as molecular chaperones and HSPB6 also has a molecular chaperone activity. HSPB6 is up-regulated in response to diverse cellular stress or damage and protect cells from otherwise lethal conditions. HSPB6 is widely recognized as a principle mediator of cardioprotective signaling and recent studies have unraveled the protective role of HSPB6 in disease or injury to the central nervous system. Moreover, accumulating evidence has implicated HSPB6 as a key mediator of diverse vital physiological processes, such as smooth muscle relaxation, platelet aggregation. The versatility of HSPB6 can be explained by its direct involvement in regulating different client proteins and its ability to form heterooligomer with other sHSPs, which seems to be dependent on HSPB6 phosphorylation. This review focuses on the properties including expression and regulation pattern, phosphorylation, chaperon activity, multiple cellular targets of HSPB6, as well as its possible role in physical and pathological conditions.

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Section: Role Of Hspb6 In Cancermentioning

confidence: 96%

Study of HSPB6: Insights into the Properties of the Multifunctional Protective Agent

Xiao

Zhou

et al. 2017

Cell Physiol Biochem

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“…Generated IPA networks were ranked by statistical 'score' based upon the inclusion of as many proteins from the inputted list as possible. For more details on IPA network scores and features, please refer to references [11,12] or Ingenuity Systems Ltd online help manual (https://analysis. ingenuity.com/pa/info/help/help.htm#ipa-help.htm).…”

Section: Systematic Review Of Meningioma Proteomicsmentioning

confidence: 99%

Proteomic data in meningiomas: post-proteomic analysis can reveal novel pathophysiological pathways

et al. 2011

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Meningiomas account for approximately 20% of adult primary intracranial tumours. WHO I meningiomas are the most common and are generally benign, but can progress, recur or transform to WHO II or WHO III grades over many years. A systematic review of multiple independent shotgun proteomic analyses of meningioma was performed to provide insight into underlying disease pathways. Shotgun proteomics has been conducted in seven meningioma related studies but there is considerable variation in aims, methodology, statistical power and the use of control tissue between these studies. Fifteen proteins which are different between WHO I and WHO II meningiomas and nine proteins which are different between WHO II and WHO III meningiomas have been described but without a view of their biological significance. Network analysis of proteins different between WHO I and WHO II meningiomas provided a coherent hypothesis for the involvement of these proteins in meningioma. Western blot analyses of meningioma tissue provided a measure of support for a core component in the network (involving VDAC2, APOA1 and HNF4α) but highlighted intrinsic difficulty of proteomic and biochemical analysis of meningiomas (as a consequence of gross alterations in tissue composition). Systematic review of shotgun proteomics and network analysis provides insight into meningioma pathophysiology despite the many barriers and difficulties that are inherent to this type of study.

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“…Both the inspection of physical properties of genes, transcripts and proteins as well as the analysis of gene ontology and the functional interaction and cluster analysis in neuroproteomics are required to produce interpretation of proteomic data and further expand our knowledge about the complexity of the brain [96]. There have been a variety of bioinformatics tools used for this purpose, for example, the key proteomics databases PRIDE [97] and BrainProfilDB [98], which aimed to provide a database system for integrating large sets of high-throughput functional genomic data of the Human Brain Proteome Project (HBPP).…”

Section: Comparative Analysis and Integration Of Transcriptomic And Pmentioning

confidence: 99%

Proteomic Studies on the Development of the Central Nervous System and Beyond

Zhang

2010

Neurochem Res

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Neuroproteomics has become a 'symbol' or even a 'sign' for neuroscientists in the post-genomic era. During the last several decades, a number of proteomic approaches have been used widely to decipher the complexity of the brain, including the study of embryonic stages of human or non-human animal brain development. The use of proteomic techniques has allowed for great scientific advancements, including the quantitative analysis of proteomic data using 2D-DIGE, ICAT and iTRAQ. In addition, proteomic studies of the brain have expanded into fields such as subproteomics, synaptoproteomics, neural plasma membrane proteomics and even mitochondrial proteomics. The rapid progress that has been made in this field will not only increase the knowledge based on the neuroproteomics of the developing brain but also help to increase the understanding of human neurological diseases. This paper will focus on proteomic studies in the central nervous system and especially those conducted on the development of the brain in order to summarize the advances in this rapidly developing field.

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The utility of functional interaction and cluster analysis in CNS proteomics

Cited by 9 publications

References 28 publications

Study of HSPB6: Insights into the Properties of the Multifunctional Protective Agent

Study of HSPB6: Insights into the Properties of the Multifunctional Protective Agent

Proteomic data in meningiomas: post-proteomic analysis can reveal novel pathophysiological pathways

Proteomic Studies on the Development of the Central Nervous System and Beyond

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