The utility of Ki-67 expression in the differential diagnosis of prostatic intraepithelial neoplasia and ductal adenocarcinoma

Rioux‐Leclercq, Nathalie; Leray, Emmanuelle; Patard, Jean Jacques; Lobel, Bernard; Guilhot, François; Jouan, Florence; Bellaud, Pascale; Epstein, Jonathan I.

doi:10.1016/j.humpath.2005.01.021

Cited by 16 publications

(8 citation statements)

References 30 publications

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“…Another difference in immunophenotype is in Ki-67 labeling, in which the Ki-67 defined proliferation index for ductal adenocarcinoma is high, with a mean of 33%, compared with usual acinar adenocarcinoma, at a mean of 8%. 25 In a small series, ductal adenocarcinomas displayed more frequent cytokeratin 20 expression. 16 Immunohistochemical staining for other proteins, including estrogen receptor, androgen receptor, p53, bcl-2, and CEA, has been performed in a few ductal adenocarcinoma cases, 7,14,18,19,30 but it is not established whether there is a difference in expression compared with acinar adenocarcinoma.…”

Section: Discussionmentioning

confidence: 90%

“…Limited scale data do exist on comparative protein expression in ductal versus acinar adenocarcinoma, 7,12,[14][15][16][17][18][19][20][21][22][23][24][25] but comparative global gene expression profiling has not been performed. The aim of this study was to examine the gene expression profiles of ductal versus acinar adenocarcinoma to assess the molecular relatedness of ductal and acinar adenocarcinomas and to identify potential molecular differences that may explain the different histopathological features and disparate clinical behavior of ductal adenocarcinoma of the prostate.…”

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confidence: 99%

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Gene expression profiles of ductal versus acinar adenocarcinoma of the prostate

et al. 2009

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Ductal adenocarcinoma is an uncommon variant of prostatic adenocarcinoma with a generally more aggressive clinical course than usual acinar adenocarcinoma. However, the molecular distinction between ductal and acinar adenocarcinomas is not well characterized. The aim of this investigation was to evaluate the relatedness of ductal versus acinar prostatic adenocarcinoma by comparative gene expression profiling. Archived, deidentified, snap frozen tumor tissue from 5 ductal adenocarcinomas, 3 mixed ductal-acinar adenocarcinomas, and 11 acinar adenocarcinomas cases were analyzed. All cases of acinar and ductal adenocarcinomas were matched by Gleason grade. RNA from whole tissue sections of the 5 ductal and 11 acinar adenocarcinomas cases were subjected to gene expression profiling on Affymetrix U133Plus2 microarrays. Independently, lasercapture microdissection was also performed on the three mixed ductal-acinar cases and five pure acinar cases to isolate homogeneous populations of ductal and acinar carcinoma cells from the same tumor. Seven of these laser-capture microdissected samples (three ductal and four acinar cell populations) were similarly analyzed on U133Plus2 arrays. Analysis of data from whole sections of ductal and acinar carcinomas identified only 25 gene transcripts whose expression was significantly and at least two-fold different between ductal and acinar adenocarcinomas. A similar analysis of microdissected cell populations identified 10 transcripts, including the prolactin receptor, with more significant differences in expression of 5-to 27-fold between ductal and acinar adenocarcinomas cells. Overexpression of prolactin receptor protein in ductal versus acinar adenocarcinoma was confirmed by immunohistochemistry in an independent set of tumors. We conclude that ductal and acinar adenocarcinomas of the prostate are strikingly similar at the level of gene expression. However, several of the genes identified in this study, including the prolactin receptor, represent targets for further investigations on the molecular basis for histomorphological and clinical behavioral differences between acinar and ductal adenocarcinomas.

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Section: Discussionmentioning

confidence: 90%

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confidence: 99%

Gene expression profiles of ductal versus acinar adenocarcinoma of the prostate

et al. 2009

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“…Other patterns, including the tall columnar epithelial pattern and a ‘PIN-like ductal adenocarcinoma’ pattern, have also been described [2,9]. The differential diagnosis includes prostatic urethral polyps, hyperplastic benign prostate glands, high-grade PIN (prostatic intraepithelial neoplasia), metastatic colorectal adenocarcinoma and papillary urothelial carcinoma [10]. The first three entities do not exfoliate easily and are not usually found in urine specimens.…”

Section: Discussionmentioning

confidence: 99%

Urinary Cytology of Prostatic Duct Adenocarcinoma – A Clinicopathologic Analysis

Sathiyamoorthy

Ali²

2013

Acta Cytologica

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Objective: To review atypical/positive urine cytology specimens from patients with confirmed prostatic duct adenocarcinoma (PDA), describe the cytomorphologic features and discuss the differential diagnosis. Study Design: A retrospective review of the cytopathology archives of The Johns Hopkins Hospital revealed 14 cases of abnormal urinary cytology in patients with histologically confirmed PDAs. Cytologic material was reviewed; cytomorphologic characteristics were analyzed and correlated with histopathologic features wherever possible. Results: There were 7 patients ranging in age from 52 to 80 years (mean age: 68 years). The clinical presentation included; hematuria, bladder obstruction and perineal mass. The cytomorphologic characteristics were: large crowded sheets and three-dimensional fragments (some with papillary architecture) of columnar epithelium with scant amphophilic cytoplasm, basally placed round to oval nuclei with coarse chromatin and absent or inconspicuous nucleoli. Histologic findings showed papillary and cribriform architecture of columnar cells with crowding, nuclear stratification and overlapping with or without nucleoli. Conclusion: PDAs usually present at an advanced clinical stage and may exfoliate owing to their location in the central prostatic urethra. Differentiating PDA from acinar prostatic adenocarcinoma, urothelial carcinoma and metastatic adenocarcinoma is important due to the differences in patient management. Since prostate-specific antigen values are variable, urine cytology may provide a valuable tool for the early detection of these aggressive lesions.

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“…Ki67 is a nuclear marker used for assessing the proliferative index in a neoplastic cell population (all stages in the cell cycle except G 0 ). The establishment of the growth fraction of neoplasms by quantitatively identifying Ki67-positive cells by immunohistochemistry is useful for the diagnosis (differential or not) of a great number of neoplastic diseases such as lymphoproliferative disorders, breast cancer and other carcinomas [26][27][28][29][30][31]. The staining pattern of proliferating cells has been also demonstrated to be important for the determination of the nuances and pitfalls that may exist in the interpretation of the Ki67 pattern [26].…”

Section: Discussionmentioning

confidence: 99%

JPEG2000 for automated quantification of immunohistochemically stained cell nuclei: a comparative study with standard JPEG format

et al. 2010

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The Joint Photographic Experts Group (JPEG) standard format is one of the most widely used in image compression technologies. More recently, JPEG2000 format has emerged as a state-of-the-art technology that provides substantial improvements in picture quality at higher compression ratios. However, there has been no attempt to date to determine which of the two compression formats produces less variability in the automated evaluation of immunohistochemically stained digital images in agreement with their compression rates and complexity degrees. The evaluation of Ki67 and FOXP3 immunohistochemical nuclear markers was performed in a total of 329 digital images: 47 were captured in uncompressed Tagged Image File Format (TIFF), 141 were converted to three JPEG compressed formats (47 each with 1:3, 1:23 and 1:46 compression) and 141 were converted to three JPEG2000 compressed formats (47 each with 1:3, 1:23 and 1:46 compression). The count differences between images in TIFF versus JPEG formats were compared with those obtained between images in TIFF versus JPEG2000 formats at the three levels of compression. It was found that, using JPEG2000 compression, the results of the stained nuclei count are close enough to the results obtained with uncompressed images, especially in highly complex images at minimum and medium compression. Otherwise, in images of low complexity, JPEG and JPEG2000 had similar count efficiency to that of the original TIFF images at all compression levels. These data suggest that JPEG2000 could give rise to an efficient means of storage, reducing file size and storage capacity, without compromise on the immunohistochemical analytical quality.

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The utility of Ki-67 expression in the differential diagnosis of prostatic intraepithelial neoplasia and ductal adenocarcinoma

Cited by 16 publications

References 30 publications

Gene expression profiles of ductal versus acinar adenocarcinoma of the prostate

Gene expression profiles of ductal versus acinar adenocarcinoma of the prostate

Urinary Cytology of Prostatic Duct Adenocarcinoma – A Clinicopathologic Analysis

JPEG2000 for automated quantification of immunohistochemically stained cell nuclei: a comparative study with standard JPEG format

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