Eighty-five accessions of Buffalo gourd, Cucurbita foetidissima HBK., were assayed for variation in seven fruit and seed characteristics: fruit diameter (s = 65 mm); seed weight per 100 seeds (s = 3.8 g); seed weight per fruit (~ = 8.4 g); seed number per fruit (~ = 225); percentage embryo in seed (~ = 67.3%); percent crude fat of whole seed (K = 32.9%); and percent crude protein of defatted embryo (~ = 69.5%). The variation in amino acid distribution (12) accessions) and in oil composition (15 accessions) was obtained. Relationships among traits were explored. Agronomic improvement through breeding was also discussed.