The vacuolar serine protease, a cross-reactive allergen from Cladosporium herbarum

Pöll, V.; Denk, Ursula; Shen, Horng-Der; Panzani, R; Dissertori, Oliver; Lackner, Peter; Hemmer, Wolfgang; Mari, Adriano; Crameri, Reto; Lottspeich, Friedrich; Rid, Raphaela; Richter, Klaus; Breitenbach, Michael; Simon-Nobbe, Birgit

doi:10.1016/j.molimm.2008.11.017

Cited by 35 publications

(21 citation statements)

References 52 publications

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“…Furthermore, IgE binding of rPer a 10 was completely inhibited when pooled patients' sera were preabsorbed with 500 g of self-protein in immunoblot inhibition, and there was no inhibition in C. lunata serine protease, which showed clear bands. Serine proteases are reported from many sources, and most of them are cross-reactive allergens (22)(23)(24).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Recombinant Per a 10 from Periplaneta americana

Govindaraj

Gaur

Arora

2013

Clin Vaccine Immunol

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bCockroach allergen is a major risk factor for IgE-mediated allergic response and asthma in sensitized individuals. Serine proteases have been identified from various sources and characterized as major allergens. The present study was aimed to express and characterize recombinant allergen Per a 10 (rPer a 10) from Periplaneta americana. rPer a 10 was expressed in Escherichia coli and purified in soluble form, yielding 0.75 mg/liter of culture. Homology of the Per a 10 protein sequence exhibited 27 to 38% similarity to the mite serine protease and 41 to 52% similarity to other insect trypsins. The purified rPer a 10 protein resolved at 28 kDa on SDS-PAGE and was recognized by cockroach-hypersensitive patients' sera by immunoblotting and enzymelinked immunosorbent assay (ELISA). In competitive ELISA, rPer a 10 required 96 ng of purified protein for 50% inhibition of IgE binding, whereas 34 ng of native protein (nPer a 10) was required for the same inhibition. rPer a 10 and nPer a 10 induced basophil histamine release in the range of 47 to 64% and 60 to 85%, respectively, when sensitized with cockroach-hypersensitive patients' sera. In conclusion, Per a 10 was subcloned, and the protein was purified to homogeneity. rPer a 10 showed reduced IgE binding and histamine release and showed no proteolytic activity. These data suggest that rPer a 10 has potential for immunotherapy.

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Section: Discussionmentioning

confidence: 99%

Characterization of Recombinant Per a 10 from Periplaneta americana

Govindaraj

Gaur

Arora

2013

Clin Vaccine Immunol

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“…However, recombinant serine protease lacked proteolytic activity. Most of the subtilisin like serine proteases are produced as precursor molecules where signal peptide helps in membrane translocation whereas prosequence functions as self-inhibitor (Pöll et al, 2009). It will be a challenge to activate Cur l 4 to have enzymatic activity by removing the prosequence.…”

Section: Discussionmentioning

confidence: 99%

“…A study showed that serine protease levels in the environment were linked to incidence of asthma (Montealegre et al, 2004). Alkaline and vacuolar serine proteases were major allergens of fungi such as Aspergillus, Cladosporium, Penicillium, Rhodotorula and Trichophyton (Pöll et al, 2009). Proteolytic activity increases bronchial epithelial permeability by degrading proteins of desmosomes, tight and adherens junctions and thereby facilitating antigen access to the immune system (Robinson et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Proteolytic activity increases bronchial epithelial permeability by degrading proteins of desmosomes, tight and adherens junctions and thereby facilitating antigen access to the immune system (Robinson et al, 2001). Fungal serine proteases such as Asp fl 1, Cla c 9, Cla h 9, Pen ch 13, Pen ch 18 and Pen o 18 were cloned and expressed as recombinant proteins (Yu et al, 1999;Shen et al, 2001Shen et al, , 2003Chou et al, 2002Chou et al, , 2008Pöll et al, 2009). These homogeneous preparations can be modified by reducing allergenic activity to foster advantageous immunologic properties like abrogating IgE binding epitopes or tagging novel sequences for obtaining better immunotherapeutic response.…”

Section: Introductionmentioning

confidence: 99%

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Molecular and immunological characterization of subtilisin like serine protease, a major allergen of Curvularia lunata

et al. 2011

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“…Another example is the cross-reaction of Curvularia lunata major allergen Cur 13 with several fungi and grasses [48]. To summarize, a large number of recombinant mold allergens have similarities to one or several other fungal enzymes [49][50][51]. Fungal allergens have been recently characterized by specifying whether or not the respective cross-reactive allergen may be found within one fungal phylum, all fungal phyla, or even non-fungal species [52].…”

Section: Discussionmentioning

confidence: 99%

Cutaneous yeast microflora in patients with atopic dermatitis

Zinkevičienė¹,

Vaiciulioniene²,

Baranauskiene³

et al. 2011

Open Medicine

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AbstractThe skin of persons with atopic dermatitis (AD) is very susceptible to cutaneous infection, and some yeast species may also aggravate AD. The total yeast population of an AD patient’s skin and its relation with individual age and body part remains poorly characterized. The aim of this study was to clarify the differences in cutaneous yeast flora by age and body parts of AD patients.By swabbing affected body parts (hands, legs, face, neck or trunk), 241 samples were collected from patients with AD (132 children and 109 adults), and as controls, 40 samples were taken from healthy individuals (20 children, 20 adults).In all, 89 (36.9%) of samples were positive; the yeast isolated belonged to three genera: Candida (27.4%), Malassezia (6.6%), and Rhodotorula (2.9%). Cutaneous colonization with yeasts was two-fold higher in the adults than in children (P<0.0001). The distribution of the yeast species was dependent on the body part sampled: Malassezia predominated in the face, neck, and trunk regions (P=0.0047); Candida more frequently colonized hands and legs (P=0.0029).Our study showed that cutaneous yeast flora and distribution of yeast species depends significantly on the age of the AD patient and the body part affected by atopic dermatitis.

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The vacuolar serine protease, a cross-reactive allergen from Cladosporium herbarum

Cited by 35 publications

References 52 publications

Characterization of Recombinant Per a 10 from Periplaneta americana

Characterization of Recombinant Per a 10 from Periplaneta americana

Molecular and immunological characterization of subtilisin like serine protease, a major allergen of Curvularia lunata

Cutaneous yeast microflora in patients with atopic dermatitis

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