The value of intraoperative frozen section examination of sentinel lymph nodes in breast cancer

Vrande, Simon L.B. Van de; Klinkenbijl, Jean H. G.

doi:10.1016/s1359-6349(08)70673-6

Cited by 7 publications

(9 citation statements)

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“…Additionally, it is impossible to intraoperatively examine the entire LN using frozen sections, which may lead to false-negative results. Indeed, the sensitivity of the intraoperative frozen section for finding nodal metastases within SNs during surgery has been reported to be 47% in melanoma patients and 74% in breast cancer patients [23]. The sensitivity of touch imprint cytology is comparable to that of frozen section examination [24,25].…”

Section: Prace Oryginalnementioning

confidence: 99%

Badanie metodą jednostopniowej amplifikacji kwasu nukleinowego węzłów chłonnych w raku brodawkowatym tarczycy — porównanie z badaniem histopatologicznym i badaniem PCR w czasie rzeczywistym

Kaczka

Fendler

Borowiec

et al. 2015

Endokrynologia Polska

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First one-step nucleic acid amplification testing in papillary thyroid cancer lymph nodes -a comparison with histopathology and real-time PCRBadanie metodą jednostopniowej amplifikacji kwasu nukleinowego węzłów chłonnych w raku brodawkowatym tarczycy -porównanie z badaniem histopatologicznym i badaniem PCR w czasie rzeczywistym AbstractIntroduction: The significance of lymph node metastases and the optimal extent of lymphadenectomy remain matters of controversy in papillary thyroid cancer. This study was designed to assess the feasibility and reliability of OSNA and real-time PCR for CK19 and TG mRNA in papillary thyroid cancer lymph nodes evaluation compared to standard histopathology. Material and methods: Each of 92 randomised lymph nodes from 32 papillary thyroid cancer patients were divided into representative parts and assessed using the three studied methods. Results: Eighteen (19.6%) lymph nodes from ten (31.3%) patients were positive according to histopathology. When the cut-off value distinguishing metastatic from non-metastatic lymph nodes in the OSNA assay was set at 250 copies per microlitre, the results were positive in 16 (17.4%) lymph nodes from 11 (34.4%) patients. Twenty three (25%) lymph nodes were tested positive in real-time PCR for TG mRNA. Real-time PCR for CK19 mRNA was positive in 18 (19.6%) lymph nodes from 13 (40.6%) patients. No statistically significant differences were noted between the diagnostic accuracy of either molecular method compared to the histopathological examination (p = 0.81). Overall, 20 positive molecular biology results were noted in patients with negative histopathology results. Conversely, in 18 lymph nodes, despite a metastasis finding in histopathology, at least one molecular test yielded a negative result. Conclusions: It was revealed that OSNA is a reliable technique for the evaluation of lymph node metastases in papillary thyroid cancer. This method was shown to have equivalent accuracy to histopathology and real-time PCR. (Endokrynol Pol 2014; 65 (6): 422-430)

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Section: Prace Oryginalnementioning

confidence: 99%

Badanie metodą jednostopniowej amplifikacji kwasu nukleinowego węzłów chłonnych w raku brodawkowatym tarczycy — porównanie z badaniem histopatologicznym i badaniem PCR w czasie rzeczywistym

Kaczka

Fendler

Borowiec

et al. 2015

Endokrynologia Polska

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“…Frozen section histology is the main alternative to touch imprint cytology, is employed in centers in other parts of the world, and achieves 67 to 75% sensivity and 100% specificity, 7,8 dependent on whether the cancer is lobular or ductal. Clearly, ESS needs to be able to compete with these techniques if it is to be of interest to clinicians.…”

Section: Discussionmentioning

confidence: 99%

“…Currently, intraoperative diagnosis is carried out using either touch imprint cytology ͑TIC͒ 6 the microscopic examination of cells obtained by pressing the cut surface of the node onto a glass microscope slide, or frozen section histopathology, 7,8 where the node is snap-frozen and cut within minutes of excision. Both of these techniques require tissue preparation and a skilled, specialized pathologist to be available in real time to provide analysis.…”

Section: Introductionmentioning

confidence: 99%

Scanning elastic scattering spectroscopy detects metastatic breast cancer in sentinel lymph nodes

Austwick

2010

J. Biomed. Opt

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Abstract.A novel method for rapidly detecting metastatic breast cancer within excised sentinel lymph node͑s͒ of the axilla is presented. Elastic scattering spectroscopy ͑ESS͒ is a point-contact technique that collects broadband optical spectra sensitive to absorption and scattering within the tissue. A statistical discrimination algorithm was generated from a training set of nearly 3000 clinical spectra and used to test clinical spectra collected from an independent set of nodes. Freshly excised nodes were bivalved and mounted under a fiber-optic plate. Stepper motors rasterscanned a fiber-optic probe over the plate to interrogate the node's cut surface, creating a 20ϫ 20 grid of spectra. These spectra were analyzed to create a map of cancer risk across the node surface. Rules were developed to convert these maps to a prediction for the presence of cancer in the node. Using these analyses, a leave-one-out crossvalidation to optimize discrimination parameters on 128 scanned nodes gave a sensitivity of 69% for detection of clinically relevant metastases ͑71% for macrometastases͒ and a specificity of 96%, comparable to literature results for touch imprint cytology, a standard technique for intraoperative diagnosis. ESS has the advantage of not requiring a pathologist to review the tissue sample.

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“…Intraoperative sentinel lymph node (SLN) biopsy is a good predictor of the axillary nodal status and is now a standard method in the assessment of patients with early breast cancer [3][4][5][6]. Despite the general acceptance of the SLN model, protocols for intra-and postoperative processing of SLN differ widely [7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…The accuracy of IFS and the rate of false negative intraoperative results depend on the intraoperative work-up of the SLN and the size of the metastasis. Some studies have demonstrated a good accuracy of IFS for macro-metastases, but not for small metastases or isolated tumor cells [3,6].…”

Section: Introductionmentioning

confidence: 99%

Pathological Processing Techniques and Final Diagnosis of Breast Cancer Sentinel Lymph Nodes

et al. 2010

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The value of intraoperative frozen section examination of sentinel lymph nodes in breast cancer

Cited by 7 publications

References 0 publications

Badanie metodą jednostopniowej amplifikacji kwasu nukleinowego węzłów chłonnych w raku brodawkowatym tarczycy — porównanie z badaniem histopatologicznym i badaniem PCR w czasie rzeczywistym

Badanie metodą jednostopniowej amplifikacji kwasu nukleinowego węzłów chłonnych w raku brodawkowatym tarczycy — porównanie z badaniem histopatologicznym i badaniem PCR w czasie rzeczywistym

Scanning elastic scattering spectroscopy detects metastatic breast cancer in sentinel lymph nodes

Pathological Processing Techniques and Final Diagnosis of Breast Cancer Sentinel Lymph Nodes

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