THE VALUE OF ISOTYPE DETERMINATION OF SERUM ANTIBODIES AGAINST <i>CHLAMYDIA</i> FOR THE DIAGNOSIS OF <i>CHLAMYDIA</i> REACTIVE ARTHRITIS

S, Bas; Cunningham, Tony; Tk, Kvien; Glennås, A; Melby, K; Tl, Vischer

doi:10.1093/rheumatology/35.6.542

Cited by 20 publications

(14 citation statements)

References 23 publications

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“…High titers of IgM antibodies to C.trachomatis have been demonstrated in only 44% of cases of perihepatitis with findings of PID (Fitz-Hugh-Curtis syndrome) by one author [13]. ELISA test of patients suspected to have C.trachomatis infection has recently been used as a diagnostic tool [14][15][16][17][18][19]. Hence we used this test to demonstrate IgM antibodies in cases of melasma.…”

Section: Discussionmentioning

confidence: 99%

IgM Chlamydia Trachomatis Antibodies in Cases of Melasma

Sawhney¹,

Batra

2005

Medical Journal Armed Forces India

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Section: Discussionmentioning

confidence: 99%

IgM Chlamydia Trachomatis Antibodies in Cases of Melasma

Sawhney¹,

Batra

2005

Medical Journal Armed Forces India

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“…Sensitivity, specificity, positive predictive value, negative predictive value, and false-positive and false-negative rates were calculated as described previously (1).…”

Section: Methodsmentioning

confidence: 99%

Chlamydial Serology: Comparative Diagnostic Value of Immunoblotting, Microimmunofluorescence Test, and Immunoassays Using Different Recombinant Proteins as Antigens

Bas

Muzzin²,

Ninet

et al. 2001

J Clin Microbiol

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To improve the reliability of the serodiagnosis of Chlamydia trachomatis infections, an immunoblot analysis, a microimmunofluorescence titration, and different immunoassays using synthetic peptides derived from species-specific epitopes in variable domain IV of the major outer membrane protein or recombinant antigens (heat shock protein 70 [hsp70], hsp60, hsp10, polypeptide encoded by open reading frame 3 of the plasmid [pgp3], macrophage infectivity potentiator, and a fragment of the total lipopolysaccharide) were evaluated. Because cross-reactions between chlamydial species have been reported, the microimmunofluorescence tests were also performed with Chlamydia pneumoniae and Chlamydia psittaci used as antigens, and C. pneumoniaespecific antibodies were also determined by immunoassays. Since the presence of antimicrobial antibodies must be interpreted in light of their prevalence in the general population, responses obtained with serum samples from patients with well-defined infection (i.e., with positive urethral or endocervical C. trachomatis DNA amplification) were compared to those obtained with samples from healthy blood donors. The best sensitivity (86%) with a specificity of 81% was obtained for immunoblotting results, when the number of individuals with >10 immunoglobulin G (IgG) and/or >2 IgM responses to the different C. trachomatis antigens was considered. A 13-kDa antigen was recognized by most of the samples (86% for IgG) from patients with acute urogenital infection but rarely (3%) by those from healthy blood donors (P < 0.0001). The sensitivity and specificity results obtained for serum antibodies to peptides or recombinant antigens were slightly lower than those results obtained for the number of responses to whole C. trachomatis antigens, which were 76 and 77%, respectively, when IgG responses to both recombinant hsp60 and pgp3 were considered.

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“…Sensitivity, specificity, positive predictive value, negative predictive value, false-positive and false-negative rates, and agreement were calculated as described previously (2).…”

Section: Methodsmentioning

confidence: 99%

Chlamydia trachomatis Serology: Diagnostic Value of Outer Membrane Protein 2 Compared with That of Other Antigens

S¹,

Muzzin²,

Vischer³

2001

J Clin Microbiol

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Different immunoassays using recombinant antigens or synthetic peptides were evaluated for the serodiagnosis of Chlamydia trachomatis infections. Antigens used included cysteine-rich outer membrane protein 2 (OMP2), heat shock protein 60, the polypeptide encoded by open reading frame 3 of the plasmid (pgp3), synthetic peptides derived from species-specific epitopes in variable domain IV of the major OMP (MOMP) (Labsystems, Helsinki, Finland), and a fragment of the total lipopolysaccharide (Medac, Hamburg, Germany). Because cross-reactions between chlamydial species have been reported, Chlamydia pneumoniae-specific antibodies were also determined by immunoassays (Labsystems). Responses obtained with serum samples from patients with well-defined diseases (i.e., urethral or endocervical samples from which C. trachomatis DNA was amplified) were compared to those obtained with samples from healthy blood donors. The best sensitivity (79%) associated with the best specificity (82%) was obtained when immunoglobulin G (IgG) responses to both MOMP and pgp3 were considered. The highest sensitivity (89%) was obtained with anti-OMP2 IgG, but the lowest specificity (57%) was obtained with this antibody, due to probable cross-reactivity with C. pneumoniae OMP2.

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THE VALUE OF ISOTYPE DETERMINATION OF SERUM ANTIBODIES AGAINST CHLAMYDIA FOR THE DIAGNOSIS OF CHLAMYDIA REACTIVE ARTHRITIS

Cited by 20 publications

References 23 publications

IgM Chlamydia Trachomatis Antibodies in Cases of Melasma

IgM Chlamydia Trachomatis Antibodies in Cases of Melasma

Chlamydial Serology: Comparative Diagnostic Value of Immunoblotting, Microimmunofluorescence Test, and Immunoassays Using Different Recombinant Proteins as Antigens

Chlamydia trachomatis Serology: Diagnostic Value of Outer Membrane Protein 2 Compared with That of Other Antigens

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