The Value of Plasmid Profiles for Strain Identification in Lactic Streptococci and the Relationship between <i>Streptoccocus lactis</i> 712, ML3 and C2

Davies, F. L.; Underwood, H. M.; Gasson, M. J.

doi:10.1111/j.1365-2672.1981.tb01249.x

Cited by 102 publications

(67 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Lactococcus lactis subsp lactis NCDü763 (CNRZ 1260) (also named MU) (Davies et al, 1981)was obtained from the CNRZ culture collection (INRA, Jouy-en-Josas, France). The TIL61 strain is a derivative of NCDü763 overexpressing the PepC aminopeptidase (Neviani et al, 1990).…”

Section: Bacterial Strainsmentioning

confidence: 99%

Phage-induced lysis of Lactococcus lactis during Saint-Paulin cheese ripening and its impact on proteolysis

Kawabata

Vassal

Bars

et al. 1997

Lait

View full text Add to dashboard Cite

Summary -The autolysis of starter bacteria during chee se ripening results in the release of intracellular enzymes, especially peptidases, into the curd and in consequence is expected to accelerate and enhance the development of cheese flavour. In the course of a series of cheese assays with the nonautolytic Lactococcus lactis NC00763 strain, the presence of a homologous bacteriophage was encountered. The phage was present at different levels which allowed us to evaluate the impact of the extent of starter bacteria Iysis on Saint-Paulin-type cheese proteolysis during ripening. We estimated bacterial Iysis by viable cell enumeration and electron microscopy observations over a 2-month ripening period. On the same cheeses, we have estimated proteolysis by measuring soluble nitrogen (SN) and phosphotun~stic acid-soluble nitrogen (PT A-N) and by quantifying free amino acids. For phage levels up to 10 0 pfu/g of cheese, the pH and dry matter values were in the standard range, whereas for a higher phage level incorrect acidification was observed. The ex te nt of starter Iysis increased with the phage level and it was also correlated with the rate of formation of small peptides and free amino acids. After 2 months of ripening, the PTA-N value was three-fold higher and the total free amino acid content was seven-fold higher in the cheese with a phage level of 10 10 pfu/g than in the control cheeses. These results confirm that the early Iysis of starter bacteria allows the release of intracellular peptidases which are active in cheese, and that the extent of starter Iysis can be correlated with the proteolysis rate.Lactococcus lactis / cheese ripening / Iysis / phage / proteolysis Résumé -Lyse de Lactococcus lactis induite par des phages au cours de l'affinage de fromages de type Saint-Paulin et impact sur la protéolyse.L'autolyse des bactéries lactiques du levain résulte en la libération des enzymes intracellulaires, en particulier des peptidases, dans le

show abstract

Section: Bacterial Strainsmentioning

confidence: 99%

Phage-induced lysis of Lactococcus lactis during Saint-Paulin cheese ripening and its impact on proteolysis

Kawabata

Vassal

Bars

et al. 1997

Lait

View full text Add to dashboard Cite

show abstract

“…lactis strains 7 12. C2 and ML3 (Davies et al, 1981). The gene for bacteriophage lysin has been cloned and sequenced (Shearman et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the prolate-headed lactococcal bacteriophage OvML3: location of the lysin gene and its DNA homology with other prolate-headed phages

Shearman

Hertwig

Teuber

et al. 1991

Journal of General Microbiology

Self Cite

View full text Add to dashboard Cite

4vML3 is a virulent prolate-headed bacteriophage that attacks a genetically well-studied strain family including Lactococcus Iactis subsp. Iactis strains 712, C2 and ML3. A restriction map was constructed for &ML3 using a wide variety of restriction endonucleases. The DNA was highly refractory to in uitro restriction; this is a common feature of lytic lactococcal phages. Genome size was estimated as 23 kb, which is similar to the sizes of other phages of the same morphological group. The presence of heat-dissociable DNA fragments was noted in gel electrophoresis of restriction enzyme digests. Cohesive ends were confirmed by pretreating the DNA with T4 ligase, which rendered the composite fragment insensitive to heat. The phage &ML3 genome thus consists of linear and non-permuted double-stranded DNA with complementary cohesive ends. The lysin gene from this phage acts as a specific lysis agent against the lactic streptococci. This gene has been cloned and sequenced previously. Further specific subcloning of EcoRV fragments of &ML3 DNA has located the lysin gene in the central region of the genome, orientated from right to left. This location was confirmed by hybridization of a lysin gene probe to @ML3 DNA. The lysin gene probe showed homology to a number of other prolate-headed phages including Pool. The lysin gene of PO01 was shown to be located in the central region of its genome. However, the isometric phage P107 did not hybridize, in spite of encoding its own lysin gene.

show abstract

“…The nine strains analyzed in this study have various genetic differences involving the presence of plasmid DNA and temperate bacteriophage (13,46) or the location of conjugative sex factor (18,47) and oligopeptide transport systems (74,78). We made the assumption that these different genetic events would produce genome rearrangements large enough to be correlated with most of the RFLPs observed above.…”

Section: Resultsmentioning

confidence: 99%

“…Strains NCDO712, NCDO505, NCDO763, MMS36, NCDO2031, and C2 are known to contain plasmid DNA (13). All these strains contain a 55-kb lactose-protease (Lac-Prt) plasmid and some other cryptic plasmids.…”

Section: Resultsmentioning

confidence: 99%

“…The L. lactis subsp. cremoris strain MG1363 (17) is a plasmid-free derivative of strain NCDO712, the ancestor of lactococcal strains NCDO763, NCDO505, NCDO2031, and C2 (13). Three other strains were also analyzed: MMS36, a recombination-deficient mutant of strain NCDO763 (1); strain LM0230, a plasmid-free derivative of strain NCDO2031 (46); and strain LM2301, a streptomycinresistant mutant of strain LM0230 (75).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Genome Plasticity among Related Lactococcus Strains: Identification of Genetic Events Associated with Macrorestriction Polymorphisms

2000

View full text Add to dashboard Cite

The genomic diversity of nine strains of the Lactococcus lactis subsp. cremoris (NCDO712, NCDO505, NCDO2031, NCDO763, MMS36, C2, LM0230, LM2301, and MG1363) was studied by macrorestriction enzyme analysis using pulsed-field gel electrophoresis. These strains were considered adequate for the investigation of genomic plasticity because they have been described as belonging to the same genetic lineage. Comparison of ApaI and SmaI genome fingerprints of each strain revealed the presence of several macrorestriction fragment length polymorphisms (RFLPs), despite a high degree of similarity of the generated restriction patterns. The physical map of the MG1363 chromosome was used to establish a genome map of the other strains and allocate the RFLPs to five regions. Southern hybridization analysis correlated the polymorphic regions with genetic events such as chromosomal inversion, integration of prophage DNA, and location of the transposon-like structures carrying conjugative factor or oligopeptide transport system.

show abstract

The Value of Plasmid Profiles for Strain Identification in Lactic Streptococci and the Relationship between Streptoccocus lactis 712, ML3 and C2

Cited by 102 publications

References 15 publications

Phage-induced lysis of Lactococcus lactis during Saint-Paulin cheese ripening and its impact on proteolysis

Phage-induced lysis of Lactococcus lactis during Saint-Paulin cheese ripening and its impact on proteolysis

Characterization of the prolate-headed lactococcal bacteriophage OvML3: location of the lysin gene and its DNA homology with other prolate-headed phages

Genome Plasticity among Related Lactococcus Strains: Identification of Genetic Events Associated with Macrorestriction Polymorphisms

Contact Info

Product

Resources

About