The value of serum survivin level in early diagnosis of cancer

Günaldı, Meral; Işıksaçan, Nilgün; Koçoğlu, Hakan; Okuturlar, Yıldız; Günaldı, Ömür; Topcu, Turkan Ozturk; Karabulut, Mehmet

doi:10.4103/0973-1482.171369

Cited by 27 publications

(12 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There has been also many studies showing that urine BIRC5 level can be used as a diagnostic indicator of bladder cancer ( Weikert et al, 2010 ) and lung cancer ( Wu et al, 2009 ). Recently, serum BIRC5 levels has been found to be positively linked to early diagnosis in majority of human cancers ( Gunaldi et al, 2018 ). Here, we proposed how BIRC5 is involved in RFWD3-mediated CRC progression.…”

Section: Discussionmentioning

confidence: 99%

RFWD3 Participates in the Occurrence and Development of Colorectal Cancer via E2F1 Transcriptional Regulation of BIRC5

Xiao

Fan

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Objectives: Colorectal cancer (CRC) is one of the most common human malignancies. It was reported that the alterations in the DNA damage response (DDR) pathways are emerging as novel targets for treatment across different cancer types including CRC. RFWD3 plays a critical role in replication protein A (RPA)-mediated DNA damage in cancer cells. More importantly, RFWD3 can response to DNA damage by positively regulating p53 stability when the G1 cell cycle checkpoint is activated. However, the functional significance of RFWD3 in CRC has not been reported in the existing documents.Materials and Methods: Here, we revealed high expression of RFWD3 in CRC tissues by IHC analysis and The Cancer Genome Atlas (TCGA) database. Besides, overexpression of RFWD3 in CRC cell lines was also confirmed by qRT-PCR and western blot assay. The Celigo cell counting method and wound-healing/transwell migration assay were applied to evaluate CRC cell proliferation and migration. The tumor growth indicators were quantified in nude mice xenografted with shRFWD3 and shCtrl RKO cells.Results: The results indicated that RFWD3 knockdown restricted CRC development in vitro and in vivo. In exploring the downstream mechanism of RFWD3’s action, we found that RFWD3 could transcriptionally activate BIRC5 by interacting with E2F transcription factor 1 (E2F1). Accordingly, we identified BIRC5 as a downstream gene of RFWD3 regulating CRC. Subsequent loss- and gain- of function experiments demonstrated that upon overexpressing BIRC5 in RKO cells with down-regulated RFWD3, the inhibitory effects of cell proliferation, migration and colony formation could be reversed, while the capacity of cell apoptosis was ameliorated, suggesting that the effects of RFWD3 depletion was mainly due to BIRC5 suppression.Conclusion: Taken together, this study revealed that RFWD3 participates in the occurrence and development of colorectal cancer via E2F1 transcriptional regulation of BIRC5.

show abstract

Section: Discussionmentioning

confidence: 99%

RFWD3 Participates in the Occurrence and Development of Colorectal Cancer via E2F1 Transcriptional Regulation of BIRC5

Xiao

Fan

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…In the human genome, the gene encoding BIRC5 is among one of the highly specific tumor genes (79) that prevent apoptosis by inhibiting the activity of different caspases (80), leading to aggressive tumor behavior and poor clinical outcomes (81). Compared to normal tissues, an increase in BIRC5 expression has been demonstrated in different types of cancers, including pancreatic (82), lung (83), colon and ovarian (84), esophageal and skin (85), colorectal and lymphoma (86) and prostate (87). In particular, in TNBC cells, the highly expressed BIRC5 is considered a marker in the early diagnosis of BC and was found to correlate with the resistance to radiation and chemotherapy (79,81,84).…”

Section: Discussionmentioning

confidence: 99%

“…Compared to normal tissues, an increase in BIRC5 expression has been demonstrated in different types of cancers, including pancreatic (82), lung (83), colon and ovarian (84), esophageal and skin (85), colorectal and lymphoma (86) and prostate (87). In particular, in TNBC cells, the highly expressed BIRC5 is considered a marker in the early diagnosis of BC and was found to correlate with the resistance to radiation and chemotherapy (79,81,84). Additionally, two more apoptosis-related genes, DAPK1 and TP73 , were significantly downregulated in GOSS-treated MM-231 and MM-468 cells, respectively (Figs.…”

Section: Discussionmentioning

confidence: 99%

Effects of gossypol on apoptosis‑related gene expression in racially distinct triple‑negative breast cancer cells

et al. 2019

View full text Add to dashboard Cite

Apoptosis is a gene‐directed mechanism that regulates cell proliferation and maintains homeostasis. However, the uncontrolled apoptotic process can lead to several pathological conditions such as tumorigenesis, and cancer metastasis. In the current study, the apoptotic effect of the natural polyphenol compound gossypol (GOSS) was investigated in the triple negative breast cancer (TNBC) cells. The effect of GOSS was evaluated in two cell lines representing Caucasian Americans and African Americans: MDA‐MB‐231 (MM‐231) and MDA‐MB‐468 (MM‐468), respectively. In both cell lines, a similar response was noticed in both cytotoxicity and proliferation studies. However, MM‐468 cells were 3‐fold more sensitive to the apoptotic effect of the compound that was accompanied by a longer delay in the colony formation. Furthermore, GOSS was found to alter the expression of many apoptosis‐related genes in our mRNA expression investigation. The compound significantly upregulated GADD45A, TNFRSF9, and BNIP3 in MM‐231 cells. Similarly, GADD45A and BNIP3 were upregulated in MM‐468. A significant finding observed in this study is the profound 159‐fold increase in the TNF gene expression that was found in MM‐468 cells. Moreover, the apoptosis‐suppressor gene, BIRC5 was significantly repressed by more than 90% in both cell lines as well as DAPK1 in MM‐231 and TP73 in MM‐468 cells. In conclusion, the data obtained in this study can provide a more understanding of the GOSS‐induced apoptosis effect and enhance the importance of this polyphenol compound as targeted towards BC treatment, particularly in African American women. Support or Funding Information This research was supported by NIH NIMHD Grants G12 MD007582 and P20 MD 006738 This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

show abstract

“…Besides breast cancer, BIRC5 detection has been related with prognosis and diagnosis of different human cancers such as acute lymphoblastic leukemia 29 , prolactinoma 30 , pancreatic cancer 31 and NSCLC 32,33 . Thus, BIRC5 overexpression is associated with adverse outcome in various cancers including breast, lung, colorectal, prostate, and ovarian cancer 34 and is considered to be one of the promising cancer diagnostic biomarker 35–38 . It has been reported that urine BIRC5 levels can serve as a diagnostic marker in case of bladder cancer 39 and quantitative detection of BIRC5 in malignant pleural effusion can be useful in lung cancer diagnosis 40 .…”

Section: Introductionmentioning

confidence: 99%

“…It has been reported that urine BIRC5 levels can serve as a diagnostic marker in case of bladder cancer 39 and quantitative detection of BIRC5 in malignant pleural effusion can be useful in lung cancer diagnosis 40 . Recently, detection of serum BIRC5 levels has been found to be positively correlated with early diagnosis of different human cancers, including breast cancer 38 .…”

Section: Introductionmentioning

confidence: 99%

Surface plasmon resonance immunosensor for label-free detection of BIRC5 biomarker in spontaneously occurring canine mammary tumours

Jena

Shrivastava

Saxena

et al. 2019

Sci Rep

View full text Add to dashboard Cite

We report detection of Baculoviral inhibitor of apoptosis repeat containing-5 (BIRC5) protein biomarker in dog serum by label-free surface plasmon resonance (SPR) immunosensor. Initially, overexpression of BIRC5 in canine mammary tumour (CMT) tissues was confirmed by real-time PCR. Recombinant BIRC5 was produced and protein specific antibodies developed in guinea pig specifically reacted with native protein in immunohistochemistry and immunocytochemistry. SPR immunosensor was developed by fabricating anti-BIRC5 antibodies on gold sensor disc. The equilibrium dissociation constant, (KD = kd/ka) was 12.1 × 10−12 M; which indicates that antibodies are of high affinity with sensitivity in picomolar range. The SPR assay could detect as low as 6.25 pg/ml of BIRC5 protein in a calibration experiment (r2 = 0.9964). On testing real clinical samples, 95% specificity and 73.33% sensitivity were recorded. The average amount of serum BIRC5 in dogs with CMT was 110.02 ± 9.77 pg/ml; whereas, in non-cancerous disease conditions, 44.79 ± 4.28 pg/ml and in healthy dog sera 30.28 ± 2.99 pg/ml protein was detected. The SPR immunosensor for detection of BIRC5 in dog sera is reported for the first time and this may find prognostic and diagnostic applications in management of CMT. In future, ‘on-site’ sensors can be developed using this technique for near-patient testing.

show abstract

The value of serum survivin level in early diagnosis of cancer

Cited by 27 publications

References 18 publications

RFWD3 Participates in the Occurrence and Development of Colorectal Cancer via E2F1 Transcriptional Regulation of BIRC5

RFWD3 Participates in the Occurrence and Development of Colorectal Cancer via E2F1 Transcriptional Regulation of BIRC5

Effects of gossypol on apoptosis‑related gene expression in racially distinct triple‑negative breast cancer cells

Surface plasmon resonance immunosensor for label-free detection of BIRC5 biomarker in spontaneously occurring canine mammary tumours

Contact Info

Product

Resources

About