The Variability of the Order Burkholderiales Representatives in the Healthcare Units

Abstract

Background and Aim. The order Burkholderiales became more abundant in the healthcare units since the late 1970s; it is especially dangerous for intensive care unit patients and patients with chronic lung diseases. The goal of this investigation was to reveal the real variability of the order Burkholderiales representatives and to estimate their phylogenetic relationships. Methods. 16S rDNA and genes of the Burkholderia cenocepacia complex (Bcc) Multi Locus Sequence Typing (MLST) scheme were used for the bacteria detection. Results. A huge diversity of genome size and organization was revealed in the order Burkholderiales that may prove the adaptability of this taxon's representatives. The following variability of the Burkholderiales in Russian healthcare units has been revealed: Burkholderiaceae (Burkholderia, Pandoraea, and Lautropia), Alcaligenaceae (Achromobacter), and Comamonadaceae (Variovorax). The Burkholderia genus was the most diverse and was represented by 5 species and 16 sequence types (ST). ST709 and 728 were transmissible and often encountered in cystic fibrosis patients and in hospitals. A. xylosoxidans was estimated by 15 genotypes. The strains of first and second ones were the most numerous. Conclusions. Phylogenetic position of the genus Lautropia with smaller genome is ambiguous. The Bcc MLST scheme is applicable for all Burkholderiales representatives for resolving the epidemiological problems.

Antibiotic treatment at delivery shapes the initial oral microbiome in neonates

“…This pattern is often regarded as a signature of dysbiosis and inflammation39. The families Bradyrhizobiaceae, Sphingomonadaceae, Comamonadaceae, Neisseriaceae and Oxalobacteriaceae are commonly isolated from hospitals404142, and harbor possible opportunistic human pathogens. In contrast, the families Streptococcaceae, Gemellaceae and order Lactobacillales were all significantly enriched in neonates whose mothers were not exposed to antibiotics.…”

Express-genotyping of Achromobacter spp. in biological samples

“…в биологиче-ском образце проводили на основе амплификации и секвенирования фрагментов гена gltB с праймера-ми, предложенными для MLST Burkholderia cepacia complex (Bcc) (табл. 1), по методике с нашими мо-дификациями [4]. Последовательности гена исполь-зовали в определении генотипов Achromobacter spp., которые нумеровали в порядке выявления, с обяза-тельной регистрацией в GenBank.…”

“…Ранее мы продемонстрировали высокую дифференцирую-щую способность фрагмента гена gltB, кодирующе-го С-концевой участок белка, в анализе генотипов Achromobacter spp. [4]. В видовой идентификации хорошо зарекомендовал себя ген оксациллиназы bla-OXA, характерный для большинства представи-телей этого рода, отличающихся резистентностью к β-лактамам [5].…”

Burkholderia contaminansBiofilm Regulating Operon and Its Distribution in Bacterial Genomes

“…Analysis of Bcc strain diversity in Russian healthcare units and in CF patients demonstrated 5 species: B. cenocepacia , B. multivorans , B. stabilis , B. contaminans , and B. vietnamiensis [1]. Among these, B. cenocepacia was more abundant, and the Russian epidemic strain ST (sequence type) 709 belonged to this species.…”

“…Among these, B. cenocepacia was more abundant, and the Russian epidemic strain ST (sequence type) 709 belonged to this species. However, B. contaminans ST102 was also isolated from CF and non-CF patients [1]. Moreover, this strain is known to have an intercontinental spread across the world [2].…”