The variation of the critical micelle concentration of sodium dodecyl sulphate with ionic strength monitored by selective-ion membrane electrodes

Newbery, J. E.

doi:10.1007/bf01474108

Cited by 19 publications

(12 citation statements)

References 7 publications

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“…At 1-mM SDS and 200 μM α-synuclein, the average micelle has 20 molecules of bound α-synuclein. At 5-mM SDS, the average micelle has only 4 molecules of bound α-synuclein (33-35, 39). In agreement with Rivers et al (38), we conclude that the micelle-bound species of α-synuclein is in a more aggregation prone conformation compared to the species present in buffer alone.…”

Section: Discussionmentioning

confidence: 99%

¹⁹F NMR Studies of α-Synuclein Conformation and Fibrillation

Li¹,

Lutz²,

Slade³

et al. 2009

Biochemistry

106

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Fibrils of the intrinsically-disordered protein α-synuclein are hallmarks of Parkinson's disease. The fluorescent dye thioflavin T is often used to characterize fibrillation, but this assay may not provide quantitative information about structure and mechanism. To gain such information, we incorporated the 19F-labeled amino acid, 3-fluorotyrosine, into recombinant human α-synuclein at its endogenous tyrosine residues. Tyrosine 39 is in the positively-charged N-terminal region of this 140-residue protein. The other three, tyrosines, 125, 133, and 136, are near the C-terminus. 19F-nuclear magnetic resonance spectroscopy was used to study several properties of labeled α-synuclein, including its conformation; conformational changes induced by urea, spermine, and sodium dodecyl sulfate (SDS); its interaction with SDS micelles; and the kinetics of fibril formation. The results show that the tyrosines are in disordered regions but that there is some structure near position 39 that is disrupted by urea. SDS binding alters the conformation near position 39, but the C-terminal tyrosines are disordered under all conditions. The NMR data also indicate that SDS-micelle bound α-synuclein and the free protein exchange on the 10-ms time scale. Studies of fibrillation show the utility of 19F-labeled NMR. The data indicate that fibrillation is not accompanied by the formation of large quantities of low molecular-weight intermediates. Although dye-binding and 19F NMR data show that 1-mM SDS and 1-mM spermine accelerate aggregation compared to buffer alone, only the NMR data indicate that the species formed in SDS are smaller than those formed in buffer or buffer plus spermine. We conclude that 19F NMR spectroscopy is useful for obtaining residue-level, quantitative information about the structure, binding, and aggregation of α-synuclein.

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Section: Discussionmentioning

confidence: 99%

¹⁹F NMR Studies of α-Synuclein Conformation and Fibrillation

Li¹,

Lutz²,

Slade³

et al. 2009

Biochemistry

106

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“…The knowledge of the cmc and hence of free energy of micelle formation, equation (16), and the measurement of the enthalpy of micellization, allow to calculate

T . Δ S_{italic micellisation}^{0}

. Figure 3 displays the data taken from the cmc measurement of Newberry [59] and the calorimetry data from Birdi [60] in the case of the most used amphiphile, sodium dodecyl sulfate (SDS).…”

Section: Some Selected Examples Of the Use Of Itc To Investigate The mentioning

confidence: 99%

“…[59] and [60]. ( ):

Δ H_{italic micellisation}^{0}

, ( ):

T . Δ S_{italic micellisation}^{0}

, (▪):

Δ G_{italic micellisation}^{0}

The dashed line corresponds to a process implying no change in energy.…”

Section: Figurementioning

confidence: 99%

Isothermal Microcalorimetry to Investigate Non Specific Interactions in Biophysical Chemistry

Ball

Maechling

2009

IJMS

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Isothermal titration microcalorimetry (ITC) is mostly used to investigate the thermodynamics of “specific” host-guest interactions in biology as well as in supramolecular chemistry. The aim of this review is to demonstrate that ITC can also provide useful information about non-specific interactions, like electrostatic or hydrophobic interactions. More attention will be given in the use of ITC to investigate polyelectrolyte-polyelectrolyte (in particular DNA-polycation), polyelectrolyte-protein as well as protein-lipid interactions. We will emphasize that in most cases these “non specific” interactions, as their definition will indicate, are favoured or even driven by an increase in the entropy of the system. The origin of this entropy increase will be discussed for some particular systems. We will also show that in many cases entropy-enthalpy compensation phenomena occur.

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“…1–5 Particularly sodium dodecyl sulfate (SDS) is used in such varied fields as cleaning applications, lubrication, stabilization of emulsions, preparation of nano- and microparticles, and even as model systems for biological membranes in protein research. 6, 7 The formation of SDS layers also raises important questions about the dynamics of molecular self-assembly on surfaces.…”

Section: Introductionmentioning

confidence: 99%

Sodium Dodecyl Sulfate Adsorption onto Positively Charged Surfaces: Monolayer Formation With Opposing Headgroup Orientations

et al. 2013

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The adsorption and structure of sodium dodecyl sulfate (SDS) layers onto positively charged films have been monitored in situ with vibrational sum-frequency-generation (SFG) spectroscopy and surface plasmon resonance (SPR) sensing. Substrates with different charge densities and polarities used in these studies include CaF2 at different pH values as well as allylamine and heptylamine films deposited onto CaF2 and Au substrates by radio frequency glow discharge deposition. The SDS films were adsorbed from aqueous solutions ranging in concentration from 0.067 to 20 mM. In general the SFG spectra exhibited well resolved CH and OH peaks. However, at SDS concentrations between 1–8 mM the SFG CH and OH intensities decreased close to background levels. Combined data sets from molecular conformation, orientation, and order sensitive SFG with mass sensitive SPR suggest that the observed changes in SFG intensities above 0.2 mM are related to structural arrangements in the SDS layer. A model is proposed where the SFG intensity minimum between 1–8 mM is associated with a monolayer containing two head group orientations, one pointing towards the substrate and one pointing towards the solution phase. The SFG peaks observed at concentrations below 0.2 mM are dominated by the presence of adsorbed contaminants such as fatty alcohols (e.g., dodecanol), which are more surface active than SDS. As SDS solution concentration is increased above 1 mM SDS molecules are incorporated in the surface layer, with dodecanol continuing to be present in the surface layer for solution concentrations up to at least critical micelle concentration.

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The variation of the critical micelle concentration of sodium dodecyl sulphate with ionic strength monitored by selective-ion membrane electrodes

Cited by 19 publications

References 7 publications

¹⁹F NMR Studies of α-Synuclein Conformation and Fibrillation

¹⁹F NMR Studies of α-Synuclein Conformation and Fibrillation

Isothermal Microcalorimetry to Investigate Non Specific Interactions in Biophysical Chemistry

Sodium Dodecyl Sulfate Adsorption onto Positively Charged Surfaces: Monolayer Formation With Opposing Headgroup Orientations

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The variation of the critical micelle concentration of sodium dodecyl sulphate with ionic strength monitored by selective-ion membrane electrodes

Cited by 19 publications

References 7 publications

19F NMR Studies of α-Synuclein Conformation and Fibrillation

19F NMR Studies of α-Synuclein Conformation and Fibrillation

Isothermal Microcalorimetry to Investigate Non Specific Interactions in Biophysical Chemistry

Sodium Dodecyl Sulfate Adsorption onto Positively Charged Surfaces: Monolayer Formation With Opposing Headgroup Orientations

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¹⁹F NMR Studies of α-Synuclein Conformation and Fibrillation

¹⁹F NMR Studies of α-Synuclein Conformation and Fibrillation