The Variations of Bacterial Community Structures in Tailing Soils Suffering from Heavy Metal Contaminations

Gao, Tianpeng; Li, Haijuan; He, Yueqing; Shen, Yuanyuan; Li, Guangwen; Li, Xiangkai; Chen, Yueli; Liu, Yubing; Liu, Changming; Ji, Jing; Xu, Jing; Chang, Guohua

doi:10.1007/s11270-021-05338-2

Cited by 21 publications

(4 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While Actinobacteriota are commonly found in mine tailings (especially metal tolerant Arthrobacter spp.) (Gao et al, 2021; Lopez et al, 2017; Xie et al, 2022), these organisms are usually obligate aerobes associated with the rhizosphere of metal hyper-accumulating plants. Therefore, we would expect to see them in the surface layers of vegetated tailings (e.g., ML34) as opposed to the deeper, ML25 tailings.…”

Section: Resultsmentioning

confidence: 99%

“…Many of the most abundant lineages are taxonomically novel and/or previously not identified within mine tailings (Figure 3). The Actinobacteriota (most abundant in ML25 but present at all locations) ASVs mostly belong to the order Gaiellales (common in deep sea sediments) (Chen et al, 2021), WCHB1-81(microcystin-contaminated lakes) (Dziga et al, 2019), and OPB41 (subsurface environments) (Khomyakova et al, 2022). Other ASVs that were unclassified at the genus/species level include the Acidobacteriaceae (associated with mine tailings but highly diverse (Campbell, 2014), as shown by the fact that the two subgroups identified in this study exhibit very different localization patterns), as well as the Gemmatimonadaceae, Pirellulaceae, Comamonadaceae, Chitinophagaceae, and Caulobacteraceae, which have not previously been associated with mine tailings.…”

Section: ) Most Abundant In Ml34;mentioning

confidence: 99%

See 1 more Smart Citation

Legacy copper/nickel mine tailings potentially harbor novel iron/sulfur cycling microorganisms within highly variable communities

Chen,

Grégoire,

Bain

et al. 2024

Preprint

View full text Add to dashboard Cite

The oxidation of sulfide-bearing mine tailings catalysed by acidophilic iron and sulfur-oxidizing bacteria releases toxic metals and other contaminants into soil and groundwater as acid mine drainage. Understanding the environmental variables that control the community structure and metabolic activity of endogenous microbes living in tailings (especially the abiotic stressors of low pH and high dissolved metal content) is crucial to developing sustainable bioremediation strategies. We determined the microbial community composition along two continuous vertical gradients of Cu/Ni mine tailings at each of two tailings impoundments near Sudbury, Ontario. 16S rRNA amplicon data showed high variability in community diversity and composition between locations, as well as at different depths within each location. A temporal comparison for one tailings location showed low fluctuation in microbial communities across 2 years. Differences in community composition correlated most strongly with pore-water pH, Eh, alkalinity, salinity, and the concentration of several dissolved metals (including iron, but not copper or nickel). The relative abundances of individual genera differed in their degrees of correlation with geochemical factors. Several abundant lineages present at these locations have not previously been associated with mine tailings environments, including novel species predicted to be involved in iron and sulfur cycling.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: ) Most Abundant In Ml34;mentioning

confidence: 99%

Legacy copper/nickel mine tailings potentially harbor novel iron/sulfur cycling microorganisms within highly variable communities

Chen,

Grégoire,

Bain

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…While members of the Actinobacteriota phylum display a diverse range of metabolism, the genera that are commonly found in mine tailings (especially metal tolerant Arthrobacter spp.) ( 42 – 44 ) are usually obligate aerobes associated with the rhizosphere of metal hyper-accumulating plants. Therefore, we would expect to see them in the surface layers of vegetated tailings (e.g., ML34) as opposed to the deeper, ML25 tailings.…”

Section: Resultsmentioning

confidence: 99%

Legacy copper/nickel mine tailings potentially harbor novel iron/sulfur cycling microorganisms within highly variable communities

Chen,

Grégoire,

Bain

et al. 2024

Appl Environ Microbiol

View full text Add to dashboard Cite

The oxidation of sulfide-bearing mine tailings catalyzed by acidophilic iron and sulfur-oxidizing bacteria releases toxic metals and other contaminants into soil and groundwater as acid mine drainage. Understanding the environmental variables that control the community structure and metabolic activity of microbes indigenous to tailings (especially the abiotic stressors of low pH and high dissolved metal content) is crucial to developing sustainable bioremediation strategies. We determined the microbial community composition along two continuous vertical gradients of Cu/Ni mine tailings at each of two tailings impoundments near Sudbury, Ontario. 16S rRNA amplicon data showed high variability in community diversity and composition between locations, as well as at different depths within each location. A temporal comparison for one tailings location showed low fluctuation in microbial communities across 2 years. Differences in community composition correlated most strongly with pore-water pH, Eh, alkalinity, salinity, and the concentration of several dissolved metals (including iron, but not copper or nickel). The relative abundances of individual genera differed in their degrees of correlation with geochemical factors. Several abundant lineages present at these locations have not previously been associated with mine tailings environments, including novel species predicted to be involved in iron and sulfur cycling. IMPORTANCE Mine tailings represent a significant threat to North American freshwater, with legacy tailings areas generating acid mine drainage (AMD) that contaminates rivers, lakes, and aquifers. Microbial activity accelerates AMD formation through oxidative metabolic processes but may also ameliorate acidic tailings by promoting secondary mineral precipitation and immobilizing dissolved metals. Tailings exhibit high geochemical variation within and between mine sites and may harbor many novel extremophiles adapted to high concentrations of toxic metals. Characterizing the unique microbiomes associated with tailing environments is key to identifying consortia that may be used as the foundation for innovative mine-waste bioremediation strategies. We provide an in-depth analysis of microbial diversity at four copper/nickel mine tailings impoundments, describe how communities (and individual lineages) differ based on geochemical gradients, predict organisms involved in AMD transformations, and identify taxonomically novel groups present that have not previously been observed in mine tailings.

show abstract

“…The pH had a significant positive impact on the bacterial genera of lntrasporangiaceae , Conexibacter , Limnobacter , Bacteroidetes , and Blastocatellaceae ( p < 0.01), but had a significant negative effect on Microtrichales and Saccharimonadales ( p < 0.05 or 0.01). Previous reports showed that pH was strongly positively correlated with the abundance of Conexibacter [ 45 , 46 ], Limnobacter [ 47 ], and Bacteroidetes [ 48 ], while pH was strongly negatively correlated with Saccharimonadales . Similarly, TN displayed a significant positive correlation with the genera Comamonadaceae and Anaerolineaceae .…”

Section: Resultsmentioning

confidence: 99%

Characterization of Microbial Communities in Wastewater Treatment Plants Containing Heavy Metals Located in Chemical Industrial Zones

Zeng

Wang

Song

et al. 2022

IJERPH

View full text Add to dashboard Cite

Water pollution caused by heavy metals (HMs) poses a serious risk to human health and the environment and can increase the risk of diabetes, cancer, and hypertension in particular. In this study, two full-scale wastewater treatment plants (WWTPs) in industrial zones in southern China were selected to analyze the microbial community structure, diversity, similarity, and differentiation in the anoxic/oxic (AO) and anoxic/oxic membrane bioreactor (AO-MBR) units under the stress of HMs. High-throughput sequencing showed that microbial diversity and abundance were higher in the AO process than in the AO-MBR process. In the two WWTPs, the common dominant phyla were Proteobacteria and Bacteroidetes, while the common dominant genera were Gemmatimonadaceae, Anaerolineaceae, Saprospiraceae, and Terrimonas. Manganese (Mn) and zinc (Zn) positively correlated with Saccharimonadales, Nakamurella, Micrococcales, and Microtrichales, whereas copper (Cu) and iron (Fe) positively correlated with Longilinea and Ferruginibacter. Additionally, the relative abundances of Chloroflexi, Patescibacteria, and Firmicutes differed significantly (p < 0.05) between the two processes. These results may provide comprehensive outlooks on the characterization of microbial communities in WWTPs, which could also help to reduce the potential environmental risks of the effluent from WWTPs located in industrial zones.

show abstract

The Variations of Bacterial Community Structures in Tailing Soils Suffering from Heavy Metal Contaminations

Cited by 21 publications

References 61 publications

Legacy copper/nickel mine tailings potentially harbor novel iron/sulfur cycling microorganisms within highly variable communities

Legacy copper/nickel mine tailings potentially harbor novel iron/sulfur cycling microorganisms within highly variable communities

Legacy copper/nickel mine tailings potentially harbor novel iron/sulfur cycling microorganisms within highly variable communities

Characterization of Microbial Communities in Wastewater Treatment Plants Containing Heavy Metals Located in Chemical Industrial Zones

Contact Info

Product

Resources

About