The vasopressin gene non-canonical Hogness box: effect on protein binding and promoter function

Ho, Mei Yin; Murphy, David

doi:10.1016/s0303-7207(01)00677-3

Cited by 6 publications

(5 citation statements)

References 38 publications

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“…They report osmotic regulatory sites in the AVP gene that are located in the 5′ flanking sequence between −1500 and −532 bp upstream of the TSS [56], [57], and are clearly unrelated to our observations in the core promoter domain. The only evidence that the non-canonical TATA box could be involved in regulating the AVP gene has again been shown in heterologous cell lines [58], [59] and not in MCNs. Therefore, whether the non-canonical TATA box is involved in the osmotic regulation of the OXT and AVP genes remains completely speculative.…”

Section: Discussionmentioning

confidence: 99%

Cell-Type Specific Oxytocin Gene Expression from AAV Delivered Promoter Deletion Constructs into the Rat Supraoptic Nucleus in vivo

Fields¹,

Ponzio²,

Kawasaki³

et al. 2012

PLoS ONE

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The magnocellular neurons (MCNs) in the hypothalamus selectively express either oxytocin (OXT) or vasopressin (AVP) neuropeptide genes, a property that defines their phenotypes. Here we examine the molecular basis of this selectivity in the OXT MCNs by stereotaxic microinjections of adeno-associated virus (AAV) vectors that contain various OXT gene promoter deletion constructs using EGFP as the reporter into the rat supraoptic nucleus (SON). Two weeks following injection of the AAVs, immunohistochemical assays of EGFP expression from these constructs were done to determine whether the EGFP reporter co-localizes with either the OXT- or AVP-immunoreactivity in the MCNs. The results show that the key elements in the OT gene promoter that regulate the cell-type specific expression the SON are located −216 to −100 bp upstream of the transcription start site. We hypothesize that within this 116 bp domain a repressor exists that inhibits expression specifically in AVP MCNs, thereby leading to the cell-type specific expression of the OXT gene only in the OXT MCNs.

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Section: Discussionmentioning

confidence: 99%

Cell-Type Specific Oxytocin Gene Expression from AAV Delivered Promoter Deletion Constructs into the Rat Supraoptic Nucleus in vivo

Fields¹,

Ponzio²,

Kawasaki³

et al. 2012

PLoS ONE

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“…The activation mechanism is likely to not only involve these co-operative interactions between E-boxes and the basal transcription machinery, but also interactions with other transcription factors. There are a number of potential transcription factor-binding sites in this proximal promoter region, including a GC-rich region described previously in the rat vasopressin promoter spanning k94 to k60 [11] and implicated as a repressor of the bovine vasopressin promoter [55]. Other predicted sites include LBP-1 (also known as upstream binding protein 1) and MAZ (Myc-associated zinc finger protein), both mapping to the region between the 5h ends of pAVP119 and pAVP105 (k77 to k63), and YY1.…”

Section: Discussionmentioning

confidence: 99%

“…A ' TATA ' box (CATAAATA) was originally predicted within the human vasopressin promoter [54], but has not been unequivocally shown to act as a non-canonical TATA motif. However, it was recently shown that this ' CATA ' box, although weaker than a canonical ' TATA ' box in HEK-293 cells, interacts more efficiently with an upstream enhancer and differentially binds complexes in supraoptic nucleus extracts [55]. This ' CATA ' box is present at the 5h end of the minimal pAVP65 (k23 to j42) reporter construct.…”

Section: Discussionmentioning

confidence: 99%

Upstream stimulatory factor activates the vasopressin promoter via multiple motifs, including a non-canonical E-box

Coulson

Edgson

Marshall-Jones

et al. 2003

Biochemical Journal

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We have described previously a complex E-box enhancer (-147) of the vasopressin promoter in small-cell lung cancer (SCLC) extracts [Coulson, Fiskerstrand, Woll and Quinn, (1999) Biochem. J. 344, 961-970]. Upstream stimulatory factor (USF) heterodimers were one of the complexes binding to this site in vitro. We now report that USF overexpression in non-SCLC (NSCLC) cells can functionally activate vasopressin promoter-driven reporters that are otherwise inactive in this type of lung cancer cell. Site-directed mutagenesis and electrophoretic mobility-shift analysis demonstrate that although the -147 E-box contributes, none of the previously predicted E-boxes (-147, -135, -34) wholly account for this USF-mediated activation in NSCLC. 5' Deletion showed the key promoter region as -52 to +42; however, USF-2 binding was not reliant on the -34 E-box, but on a novel adjacent CACGGG non-canonical E-box at -42 (motif E). This mediated USF binding in both SCLC and USF-2-transfected NSCLC cells. Mutation of motif E or the non-canonical TATA box abolished activity, implying both are required for transcriptional initiation on overexpression of USF-2. Co-transfected dominant negative USF confirmed that binding was required through motif E for function, but that the classical activation domain of USF was not essential. USF-2 bound motif E with 10-fold lower affinity than the -147 E-box. In NSCLC, endogenous USF-2 expression is low, and this basal level appears to be insufficient to activate transcription of arginine vasopressin (AVP). In summary, we have demonstrated a novel mechanism for USF activation, which contributes to differential vasopressin expression in lung cancer.

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“…Moreover, the luciferase activity of the 5′-flanking region containing the mutant TATA box in the promoter region was lower than that of the 5′-flanking region containing the normal TATA box. It was previously reported that the TATA box in the vasopressin and β-globin gene have a deviant CATAAAA box (Xu et al, 1991; Ho and Murphy, 2002). These mutant TATA boxes have also been shown to elicit relatively weak promoter activity (Xu et al, 1991; Ho and Murphy, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…It was previously reported that the TATA box in the vasopressin and β-globin gene have a deviant CATAAAA box (Xu et al, 1991; Ho and Murphy, 2002). These mutant TATA boxes have also been shown to elicit relatively weak promoter activity (Xu et al, 1991; Ho and Murphy, 2002). The results of the present study indicate that the TATA box in the promoter region plays an important role in expression of the β-casein gene.…”

Section: Discussionmentioning

confidence: 99%

Cloning and Molecular Characterization of Porcine β-casein Gene (CNS2)

Lee¹,

Kim²,

Moon³

et al. 2012

Asian Australas. J. Anim. Sci

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:The production of therapeutic proteins from transgenic animals is one of the most important successes of animal biotechnology. Milk is presently the most mature system for production of therapeutic proteins from a transgenic animal. Specifically, -casein is a major component of cow, goat and sheep milk, and its promoter has been used to regulate the expression of transgenic genes in the mammary gland of transgenic animals. Here, we cloned the porcine -casein gene and analyzed the transcriptional activity of the promoter and intron 1 region of the porcine -casein gene. Sequence inspection of the 5'-flanking region revealed potential DNA elements including SRY, CdxA, AML-a, GATA-3, GATA-1 and C/EBP . In addition, the first intron of the porcine -casein gene contained the transcriptional enhancers Oct-1, SRY, YY1, C/EBP , and AP-1, as well as the retroviral TATA box. We estimated the transcriptional activity for the 5'-proximal region with or without intron 1 of the porcine -casein gene in HC11 cells stimulated with lactogenic hormones. High transcriptional activity was obtained for the 5'-proximal region with intron 1 of the porcine -casein gene. The -casein gene containing the mutant TATA box (CATAAAA) was also cloned from another individual pig. Promoter activity of the luciferase vector containing the mutant TATA box was weaker than the same vector containing the normal TATA box. Taken together, these findings suggest that the transcription of porcine -casein gene is regulated by lactogenic hormone via intron 1 and promoter containing a mutant TATA box (CATAAAA) has poor porcine -casein gene activity.

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The vasopressin gene non-canonical Hogness box: effect on protein binding and promoter function

Cited by 6 publications

References 38 publications

Cell-Type Specific Oxytocin Gene Expression from AAV Delivered Promoter Deletion Constructs into the Rat Supraoptic Nucleus in vivo

Cell-Type Specific Oxytocin Gene Expression from AAV Delivered Promoter Deletion Constructs into the Rat Supraoptic Nucleus in vivo

Upstream stimulatory factor activates the vasopressin promoter via multiple motifs, including a non-canonical E-box

Cloning and Molecular Characterization of Porcine β-casein Gene (CNS2)

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