1973
DOI: 10.1113/expphysiol.1973.sp002184
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The Velocity of Erythrocytes in a Hepatic Sinusoid, and the Effect of Intraportal Injection of Adrenaline

Abstract: A method for measuring the velocity of erythrocytes in the hepatic sinusoids of the rat is described. The liver is immobilized by the use of a diaphragmatic screen and a cover glass over the anterior edge of the liver which adheres by surface tension. The liver is transilluminated by light transmitted through a flexible optical fibre guide. Erythrocyte velocity is measured by continuous photometry of the magnified microscopic image of a hepatic sinusoid; two adjacent phototransistors generate signals as the im… Show more

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Cited by 7 publications
(2 citation statements)
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“…The animal breathed spontaneously through a tracheotomy tube and the body temperature was maintained by a heating bed. As described by Cheng, Ho and Ma [1973], the anterior margin of the liver was transilluminated for direct microscopic observation through a cover-glass, using an Olympus Vanox microscope with an Oel+W 22 x /0.65 (Leitz) or an FL 40 x /0 75 (Olympus) objective, and the centre-line velocity of the erythrocytes in a hepatic microvessel or sinusoid projected on a screen was measured by a two-slit photometric method [Wayland and Johnson, 1967] with processing of the photometric signals by an on-line cross-correlation computer technique [Intaglietta, Tompkins and Richardson, 1970;Ma, Koo, Kwan and Cheng, 1974]. The diameter of the hepatic microvessels and sinusoids was measured with a caliper from enlarged photomicrographs taken serially with a camera fitted on top of the microscope and connected to an automatic exposuremeter system (Model PM-10-A, Olympus), using Kodak Plus-X film (ASA-125) and a Kodak Wratten green filter (No.…”
Section: Methodsmentioning
confidence: 99%
“…The animal breathed spontaneously through a tracheotomy tube and the body temperature was maintained by a heating bed. As described by Cheng, Ho and Ma [1973], the anterior margin of the liver was transilluminated for direct microscopic observation through a cover-glass, using an Olympus Vanox microscope with an Oel+W 22 x /0.65 (Leitz) or an FL 40 x /0 75 (Olympus) objective, and the centre-line velocity of the erythrocytes in a hepatic microvessel or sinusoid projected on a screen was measured by a two-slit photometric method [Wayland and Johnson, 1967] with processing of the photometric signals by an on-line cross-correlation computer technique [Intaglietta, Tompkins and Richardson, 1970;Ma, Koo, Kwan and Cheng, 1974]. The diameter of the hepatic microvessels and sinusoids was measured with a caliper from enlarged photomicrographs taken serially with a camera fitted on top of the microscope and connected to an automatic exposuremeter system (Model PM-10-A, Olympus), using Kodak Plus-X film (ASA-125) and a Kodak Wratten green filter (No.…”
Section: Methodsmentioning
confidence: 99%
“…and allowed to breathe spontaneously through a tracheal cannula. The anterior margin of the liver was observed microscopically by transillumination as described previously [Cheng, Ho and Ma, 1973;Koo, Liang and Cheng, 1975], and the diameter of the hepatic sinusoids was recorded by serial photomicrography using a motordriven Olympus OM-1 camera. Four to five branching sinusoids [Koo, Liang and Cheng, 1975] were randomly selected from the magnified photomicrographs and their diameters were measured with a pair of calipers.…”
mentioning
confidence: 99%