The Very Low Frequency of Epstein-Barr JC and BK Viruses DNA in Colorectal Cancer Tissues in Shiraz, Southwest Iran

Abstract

Viruses including Epstein-Barr virus (EBV), JCV and BKV have been reported to be associated with some cancers. The association of these viruses with colorectal cancers remains controversial. Our objective was to investigate their infections association with adenocarcinoma and adenomatous polyps of the colon. Totally, 210 paraffin-embedded tissue specimens encompassing 70 colorectal adenocarcinoma, 70 colorectal adenomatous and 70 colorectal normal tissues were included. The total DNA was extracted, then qualified samples introduced to polymerase chain reaction (PCR). The EBV, JCV and BKV genome sequences were detected using specific primers by 3 different in-house PCR assays. Out of 210 subjects, 98 cases were female and the rest were male. The mean age of the participants was 52 ± 1.64 years. EBV and JCV DNA was detected just in one (1.42%) out of seventy adenocarcinoma colorectal tissues. All adenomatous polyp and normal colorectal tissues were negative for EBV and JCV DNA sequences. Moreover, all the patients and healthy subjects were negative for BKV DNA sequences. The results suggested that EBV and JCV genomes were not detectable in the colorectal tissue of patients with colorectal cancer in our population. Hence, BKV might not be necessitated for the development of colorectal cancer. The findings merit more investigations.

Study on JV Virus in Patients with Colon Cancer Type Adenocarcinoma

“…In the present study 10% of CRC of patients were positive for JCV DNA and was in agreement with results reported by Mou et al, (2012) in China. Sarvari et al, (2018) have reported low prevalence of 1.42% JCV DNA in patients with CRC, in Shiraz city, Iran, which was lower than our finding. In the present study the rate of JCV DNA was among the male (7.5%) and female (2.5%) patients (p=0.29%).…”

Patient-individual cancer cell lines and tissue analysis delivers no evidence of sequences from DNA viruses in colorectal cancer cells

“…A Chinese study of 137 samples described a rate of infection in CRC of 40.9% [13]. But in contrast, a large American study failed to prove JCV infections in 233 patient samples [29] and a study from Iran described a positive detection rate of only 1.46% (2 of 70 patients) [30]. Our own results are pretty much in line with the latter ndings and also indicate that JCV is unlikely involved in CRC development.…”

Epstein-Barr virus (EBV) status in colorectal cancer: a mini review

“…The authors used PCR to examine 35 colon samples (Fifteen CRCs and twenty adenomas) and failed to detect any positive cases. Similar results were obtained in a study by Sarvari et al 19 ( Table 1).…”

“…Therefore, we believe that studies exploring EBV presence and role in colorectal adenoma-carcinoma sequence are largely missing and may significantly contribute to a better knowledge on the role of EBV in colorectal carcinogenesis. A recent study of Sarvari et al 19 revealed very low EBV positivity in invasive colorectal carcinomas (1.4%) while all corresponding colorectal adenomas (n = 70) as well as adjacent normal colonic mucosa (n = 70) were negative for EBV. Similarly, Mehrabani-Khasraghi et al 21 reported no EBV expression in any carcinomas or adenomas they investigated.…”