The viability of asparagus pollen after storage at low temperatures

Marcellán, Olga; Camadro, Elsa Lucila

doi:10.1016/s0304-4238(96)00949-1

Cited by 21 publications

(9 citation statements)

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“…Não houve diferenças significativas entre os diplóides para as concentrações de 0 e 400 mg L -1 . Dantas et al (2005), mostraram que o ácido bórico não teve efeito positivo para essa característica em experimento com maçã, enquanto Milutinovic et al (1996), obtiveram resultados positivos utilizando ácido bórico em variedades distintas de macieiras, 64,2 e 88,3% de germinação para as cultivares Jonathan e Golden Delicious, respectivamente.…”

Section: Resultsunclassified

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Viabilidade in vitro de grãos de pólen de bananeira sob diferentes concentrações de ácido bórico e sacarose

et al. 2011

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RESUMONeste trabalho, objetivou-se avaliar a germinação do grão de pólen e o comprimento do tubo polínico das bananeiras diplóides M53, 8987-01 e 9197-03, Calcutá, Lidi e 86B79-12. O delineamento utilizado foi inteiramente casualizado com cinco doses de sacarose (0, 5, 10, 15, 20%) e seis concentrações de ácido bórico (0, 100, 200, 300, 400 e 500 Mg L -1 ) com quatro repetições. Foram utilizados grãos de pólen retirados da inflorescência masculina dos diplóides Musa acuminata (AA). O pólen foi distribuído em placas de Petri, contendo o seguinte meio de cultura: 1,27 mM de Ca(NO 3 ) 2 .2H 2 O, 0,87 mM de MgSO 4 .7H 2 O, 0,99 mM de KNO 3 , 0,7% de ágar com pH ajustado para 7,0, variando as concentrações de sacarose e de ácido bórico. As avaliações foram realizadas 24 horas após a distribuição do pólen no meio de cultura. O meio de cultura padrão para germinação de grãos de pólen suplementado com 15% de sacarose proporcionou uma maior percentagem de germinação para os diplóides de bananeira avaliados. A concentração de ácido bórico adicionado ao meio de cultura para a germinação de grãos de pólen de bananeira diplóide é dependente do genótipo. Termos para indexação:Musa acuminata, diplóides, germinação, tubo polínico. ABSTRACTThe objective of this work was to evaluate the germination of pollen grain and pollen tube length of banana diploids (M53, 8987-01 e 9197-03, Calcutta, Lidi and 86B79-12). Five concentrations of sucrose (0, 5, 10, 15 and 20%) and six concentrations of boric acid (0, 100, 200, 300, 400 and 500 mg L -1 ) were used, in a completely randomized experimental design, with four replicates. The pollen was distributed in Petri dishes containing the following culture medium: 1.27 mM Ca(NO3) 2 .2H 2 O, 0.87 mM MgSO 4 .7H 2 O, 0.99 mM KNO 3 , and 0.7% agar; pH adjusted to 7.0, varying the sucrose and boric acid concentrations. The evaluations were performed 24 hours after the distribution of the pollen in the culture medium. The standard culture medium for germination of pollen grains with 15% sucrose provided the highest germination percentage for the banana diploids. The concentration of boric acid added to the culture medium for pollen grain germination of diploid banana is dependent on the genotype.

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Section: Resultsunclassified

“…A germinação in vitro é o método mais utilizado em testes de viabilidade de pólen (Marcellán & Camadro, 1996). Entretanto, o desenvolvimento desse método é influenciado por diferentes fatores, entre eles: os constituintes do meio de cultura, a temperatura e o tempo de incubação.…”

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Viabilidade in vitro de grãos de pólen de bananeira sob diferentes concentrações de ácido bórico e sacarose

et al. 2011

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“…Alexander's stain (Alexander, 1969) makes grains appear green when cytoplasm is absent. Although no correlation with germination (Marcellan and Camadro, 1996) was obtained using this stain but it can distinguish viable from non-viable pollen (Alexander, 1969). An in vitro test for pollen tube growth termed 'pollengermination' (Johri and Vasil, 1961) was made using Brewbaker's medium of different strength on pollen samples.…”

Section: Methodsmentioning

confidence: 92%

Impacts of Canopy Position, Plants’ Age and Pollinators on PrePollination Pollen Viability of Tectona grandis Linn

Bhattacharya¹

2014

IOSRJPBS

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“…In vitro germination of pollen grains has as advantages the most convenient and safe method used to test the viability of these ones (Frazon et al, 2015). For Marcellán & Camadro (1996), this method reveals the true state of the reserves, the condition of the membranes and the use of the pollen grain reserves to the germination. The in vitro germination of pollen grains is influenced by different factors, such as the species, the culture medium, the temperature and the incubation time, the stage of development of the flower when the pollen is collected, and the storage conditions (Stanley & Linskens, 1974;Sharafi, 2011).…”

Section: Introductionmentioning

confidence: 99%

Characterization of cultivars and low-temperature pollen grain storage in amaryllis (Hippeastrum sp.)

2019

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The determination of viability and the preservation of pollen grains are useful tools for breeding of ornamentals, especially when the synchronization of flowering between species or cultivars does not occur naturally. Thus, the objective of this study was to characterize the flowering and to evaluate the preservation and maintenance of the viability of pollen grains in cultivars of amaryllis (Hippeastrum sp.) subjected to three storage temperatures. Anthers of five cultivars of amaryllis were collected from 12 to 24 hours after anthesis. One part of the anthers was immediately used for germination tests using in vitro germination in culture medium and the other part was stored at temperatures of 25, 8 and-20 °C. Pollen grains were germinated in culture medium at 0, 20, 75, 125, 165, 205 and 245 days after storage. All cultivars of amaryllis showed an accelerated loss of viability at 25 °C storage temperature. The 'Bull' cultivar showed higher recalcitrance to cold-storage, while 'Intokazie' and 'Pink Panther' cultivars showed higher longevity and higher percentage of germination along storage. The temperature of-20 °C resulted in the best responses for the maintenance of the integrity and preservation of the viability of pollen grains of different cultivars of amaryllis.

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The viability of asparagus pollen after storage at low temperatures

Cited by 21 publications

References 1 publication

Viabilidade in vitro de grãos de pólen de bananeira sob diferentes concentrações de ácido bórico e sacarose

Viabilidade in vitro de grãos de pólen de bananeira sob diferentes concentrações de ácido bórico e sacarose

Impacts of Canopy Position, Plants’ Age and Pollinators on PrePollination Pollen Viability of Tectona grandis Linn

Characterization of cultivars and low-temperature pollen grain storage in amaryllis (Hippeastrum sp.)

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