The Viability of Cryopreserved Onlay Cranial Bone Allografts

Sanz, Julian; Elejabeitia, Javier; Bazán, Antonio; García-Tutor, Emilio; Paloma, V.

doi:10.1097/00000637-199604000-00008

Cited by 3 publications

(2 citation statements)

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“…Unfortunately it still took a decade before the first frozen bone banks were created [13]. Later frozen bone allografts have extensively been used in the field of craniofacial reconstructive surgery [6,7,14,23,37,41]. The use of this material for performing cranioplasties has not been previously reported.…”

Section: Discussionmentioning

confidence: 99%

“…The rate of osteogenesis extending from the bone ends must exceed the rate of fibrous tissue ingrowth [9]. Allografts do finally get incorporated into the host bone so that is impossible to distinguish them from an autograft implanted under the same conditions [9,14,36,41]. We have seen that this incorporation takes time and although is adequate, is not as perfect as normal calvarial bone.…”

Section: Discussionmentioning

confidence: 99%

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Use of frozen cranial vault bone allografts in the repair of extensive cranial bone defects

et al. 1997

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In cranioplasty complexity is proportional to the size of the detect, particularly if greater than 50 cm2. If the patient's own bone flap is not available, allogenic frozen bone graft can be used instead. Between June 1990 and June 1995 twenty cranioplasties with allogenic frozen bone grafts were performed. Age of patients ranged between 23 and 63 years (average 38.4 years). Male/female ratio was 2:1.7. Size of craniectomy ranged between 65 and 150 cm2 (average 83.3 cm2). Follow-up ranged between 10 and 58 months (average 41 months). Donors were tested to rule out transmissible diseases, infections, sepsis and/or cancer. Bone grafts were removed under aseptic conditions, microbiological cultures were taken, wrapped in a gauze soaked with Gentamicin sulphate and Bacitracin, sealed in three sterilised vinyl plastic bags, and stored in a deep freezer for a minimum of 30 days (range 36-93 days, average 67 days), at a temperature of -80 degrees C. Grafts were placed in the defect after a step was carved on its borders to facilitate the contact between host and graft. Vancomycin 1 g. IV/12 hours and Ceftriaxone 1 g. IV/12 hours were administered for five days. Grafts were covered by means of scalp flaps. Only one required a musculocutaneous free flap. None was exposed, extruded or had to be removed. Plain skull X-ray studies showed progressive remodelling of the grafts. Partial resorption was observed in two (2/20, 10%) and loss of thickness in another 3/20 (15%), but with no changes in the contour. Biopsies were taken in 3/20 (15%) cases at a second surgical procedure. Areas of osteoclastic resorptive activity mixed with others of osteoblastic bone apposition, showed replacement with new bone. We conclude that cranial vault frozen allografts are a good alternative to autologous bone when the latter is absent or not present in sufficient amount.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Use of frozen cranial vault bone allografts in the repair of extensive cranial bone defects

et al. 1997

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Radiologic and Histologic Assessment of Diced Cartilage Grafts for Cranial Bone Defects of Rabbits: An Experimental Study

et al. 2005

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This study was performed to experimentally evaluate the viability and coverage of diced cartilage grafts for cranial defects. Biparietal bone defects were prepared in each of 20 rabbits. Otogenous bone grafts were fixed to one side, whereas cartilage grafts taken from the right ear, diced, and wrapped with oxidized regenerated cellulose (Surgicel) were placed on other side. Parenchymal impression, contour, and ossification of all grafts in the 16 rabbits surviving after 8 weeks were evaluated with computed tomography and magnetic resonance imaging. The existence of ossification was examined pathologically. Parenchymal impression was significantly more frequent in bone grafts than in cartilage grafts (p < 0.05). This difference probably is attributable to the application technique. There was no significant radiographic difference in other parameters between the two groups. No significant difference in ossification was found. In conclusion, ossification was seen radiologically and pathologically in diced cartilage grafts. Because of autogenicity, ease of shaping, absence of postoperative warping, reduced resorption rates, and inherent viability and ossification properties, the authors suggest that diced cartilage grafts may be an alternative material for cranial defects, especially for frontal cranial reconstruction in which deformities may cause aesthetic and functional disabilities. The authors believe that further long-term studies also are needed.

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Cryopreservation with dimethyl sulfoxide sustains partially the biological function of osteochondral tissue

Egli

Sckell

Fraitzl

et al. 2003

Bone

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The Viability of Cryopreserved Onlay Cranial Bone Allografts

Cited by 3 publications

References 0 publications

Use of frozen cranial vault bone allografts in the repair of extensive cranial bone defects

Use of frozen cranial vault bone allografts in the repair of extensive cranial bone defects

Radiologic and Histologic Assessment of Diced Cartilage Grafts for Cranial Bone Defects of Rabbits: An Experimental Study

Cryopreservation with dimethyl sulfoxide sustains partially the biological function of osteochondral tissue

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