This experiment was designed to investigate the effect of using tuna meat as a supplement to increase testosterone hormone level in men. The experiment based on 15 non married men aged (21 – 27) divided into two groups, group of ten men who ate 100 g of tuna daily for 15 days, the other group consists of 5 men were on a control diet . Testosterone levels of all men were examined before and after by blood tests using I-chroma test procedure. The results after 15 days showed significant variant in the T hormone. For example, the treated group results before eating tuna was ( 3.22 , 2.67 , 7.38 , 2.29 , 2.91 , 2.82 , 2.52 , 2.99 , 1 , 2.54 ) ng/ml and after eating tuna is ( 2.64 , 1.94 , 7.13 , 3.47 , 6.62 , 3.68 , 3.9 , 5.32 , 2.7 , 3.5 ) ng/ml respectively. While the control group were, before: ( 4.1 , 1.06 , 3.97 , 2.15 , 1.86 ) ng/ml and after: ( 1.5 , 2 , 2.3 , 3.34 ) ng/ml respectively. The percentage of hormonal variation before and after experiment in treated group were ( -18% , -27% , -3% ) decrease for samples no. ( 1, 2, 3 ) respectively and for samples no (4, 5, 6, 7, 8, 9, 10 ) were ( 51%, 127%, 30%, 54%, 77%, 170%, 37% ) increase. While the control group have decreased levels in samples no. ( 1, 3 ) with percentage of (-14%, -49% ) respectively, and increased levels in samples number ( 2, 4, 5 ) with percentage of ( 41%, 7%, 79% ). as we notice, not much difference in control group, but treatment group has certain increase in seven samples and decrease in only three samples, because of several factors that the samples was exhibited to leads to decrease the hormone in these samples after treatment. The study concluded that , continuous oral consumption of 100g as supplement of Tuna meat for at least 15 days can lead to increase and/or regulate blood levels of testosterone hormone in men.