The vitrification method is significantly better for thawing of slow-freezing embryos

Kojima, Eigo; Fukunaga, N.; Kitasaka, Hiroya; Ohno, Hiroshi; Asada, Yoshimasa

doi:10.1016/j.fertnstert.2012.07.458

Cited by 5 publications

(1 citation statement)

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“…Since the rapid warming procedure proposed in this study is viable for oocytes, it is also potentially applicable to other less fragile reproductive cells frozen via slow freezing, such as zygotes or embryos at any stage of cleavage (from 2 cells to blastocyst). A recent prospective study performed on 359 slow-frozen pronuclear embryos in Japan confirmed the positive effect on embryo development of the rapid warming protocol: the blastocyst formation rate was significantly increased when the embryos were warmed with vitrification warming media rather than with conventional rapid thawing (Kojima et al, 2012). Furthermore, since in any protocol or commercial kit, vitrification warming solutions have a concentration of 1 mol/l (first warming step) and 0.5 mol/l (second warming step) of extracellular cryoprotectants, these solutions can be used for the warming of any reproductive cell irrespective of the freezing protocols, thus streamlining laboratory activity and potentially reducing costs.…”

Section: Discussionmentioning

confidence: 88%

Rapid warming increases survival of slow-frozen sibling oocytes: a step towards a single warming procedure irrespective of the freezing protocol?

Parmegiani

Tatone

Cognigni

et al. 2014

Reproductive BioMedicine Online

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Nowadays, human oocytes/embryos are cryopreserved via slow freezing or vitrification. The aim of this study was to evaluate a rapid warming protocol for slow-frozen human oocytes based on the standard warming procedure for vitrification. This was a prospective study on 216 sibling oocytes randomized for either conventional rapid thawing or rapid warming with vitrification warming solution. The primary endpoint was morphological assessment of survival at 2h. Surviving oocytes were divided into two subgroups: (i) parthenogenetically activated; and (ii) fixed and observed for spindle/chromosome configuration. Secondary endpoints were parthenogenetic development and spindle/metaphase configuration. Survival rate with rapid warming was higher (92/102, 90.2%) than with rapid thawing (85/114, 74.6%; P=0.005), and after 3d of culture the rapidly warmed parthenotes had more blastomeres compared with those rapidly thawed (P=0.042). Meiotic spindle and chromosomal configuration were not significantly influenced by rapid warming or rapid thawing. The finding of this study allows IVF centres to increase the efficiency of oocyte slow freezing, enabling survival rates comparable to vitrification protocols, and potentially to optimize costs by using the same warming protocol for both slow-frozen and vitrified reproductive cells.

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Section: Discussionmentioning

confidence: 88%

Rapid warming increases survival of slow-frozen sibling oocytes: a step towards a single warming procedure irrespective of the freezing protocol?

Parmegiani

Tatone

Cognigni

et al. 2014

Reproductive BioMedicine Online

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Appendix F: Quinn’s Advantage Embryo Freeze Kit

Beilby

Quinn²

2017

Methods in Molecular Biology

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Despite a large focus on the use of vitrification to cryopreserve embryos in recent years, there are still arguments for the use of slow freezing for the cleavage-stage embryo. Having said this, there are lessons to be learned from the process of vitrification that could be applied to slow freezing to improve post-thaw survival and ultimately clinical pregnancy rates. Specifically, increasing the concentration of sucrose in the freezing solution from 0.1 to 0.2 M and subsequently increasing the sucrose concentrations in thawing solutions could prove beneficial. The use of vitrification warming solutions in the thawing of slow-frozen embryos may also be an option that not only improves survival but also streamlines product purchasing and protocols within the laboratory.

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Cryopreservation of Gametes and Embryos

2019

In-Vitro Fertilization

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The vitrification method is significantly better for thawing of slow-freezing embryos

Cited by 5 publications

References 0 publications

Rapid warming increases survival of slow-frozen sibling oocytes: a step towards a single warming procedure irrespective of the freezing protocol?

Rapid warming increases survival of slow-frozen sibling oocytes: a step towards a single warming procedure irrespective of the freezing protocol?

Appendix F: Quinn’s Advantage Embryo Freeze Kit

Cryopreservation of Gametes and Embryos

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