The Water-Soluble Polysaccharides of Dermatophytes: Iii. A Galactomannan From Trichophyton Interdigitale

Abstract

The water-soluble polysaccharide preparation from Trichophyton interdigitale was fractionated to give two distinct galactomannans and a glucan. A galactomannan isolated via its insoluble copper complex had [ a ]~ +75" (water) and was composed of D-galactose (12Yq) and D-mannose (88Y0). On periodate oxidation, the galactomannan consumed 1.73 mole perlodate and released 0.67 mole formic acid and 0.12 mole formaldehyde per anhydrohexose unit. Hydrolysis of the methylated galactomannan gave 2,3,5,6-tetra-0-methyl-D-galactose (1 part), 2,3,4,6-tetra-O-methyl-~-n1annose (1 part), 2,3,4-tri-0-methyl-D-mannose (4 parts), and 3,4-di-0-methyl-D-mannose (2 parts). Mild acid hydrolysis of the galactomannan removed all the galactose residues, leaving a mannan having [ a ]~ +84O (water) whose structure was analyzed b y periodate oxidation and methylation techniques.Previous papers in this series have described the structural features of polysaccharides isolated from Trichophyton granulosum (I) and Microsporum quinckeanum (2). The watersoluble polysaccharides of derinatophytes form a part of the antigenic materials of these organisins (3) and as part of a prograin directed towards a study of the immunological properties of a group of related dermatophytes, the water-soluble polysaccharides isolated from Triclzophyton interdigitale Priestly, 1917, have now been investigated. This paper records the structural analysis of a galactomannan isolated via its insoluble copper coinplex from the crude polysaccharide preparation obtained on extraction of T. interdigitale \\-ith 3y0 sodium hydroxide solution.The polysaccharides were extracted in about By0 yield from the powdered cells of T. interdigitale in the way described previously for T. granulosum (I). The product was a light-broxvn powder containing 0.6y0 nitrogen and 5.1y0 ash and on hydrolysis gave mannose, glucose, and galactose in the approximate ratio of 5: 1: 1. i\iIoving boundary electrophoresis of the polysaccharides in 0.05 M sodium tetraborate solution showed it to be coinposed of a t least three components having the mobilities 8.43 X 8.19 X and 3.83 X lop5 cin2/V s. The preparation was fractionated according to the scheme outlined in Fig. 1.The polysaccharide obtained via its insoluble copper complex (galactomannan I) on moving boundary electrophoresis in 0.05 M sodium tetraborate gave a single sharp symmetrical peak (Fig. 2) having the same mobility as the fastest moving component in the original polysaccharide mixture. The non-copper complexing polysaccharides were recovered by ethanol precipitation froin the deionized Fehling solution and were then resolved by chromatography on diethylaminoethyl (DEAE) cellulose (4). A glucan, which had [oIDe1 -16' (water) and on moving boundary electrophoresis in 0.05 M sodium

The Chemistry of Polysaccharides of Fungi and Lichens

“…The first detailed investigation concerned the mycelia of various dermatophytes: Trichophyton granulosum, Trichophyton interdigitale, Trichophyton rubrum, Trichophyton schönleinii, and Microsporum quickeanum (233)(234)(235)(236)(237)(238). Poly saccharides containing D-galactose residues in both furanose and pyranose ring forms, and for the latter both a-D and β-Ό configurations, have been encountered.…”

Immunological Studies on Dermatophytes II. Serological Reactivities of Mannans Prepared from Galactomannans I and II of Microsporum quinckeanum, Trichophyton granulosum, Trichophyton interdigitale, Trichophyton rubrum , and Trichophyton schoenleinii

“…The isolation of the polysaccharides and the elucidation of their gross chemical structures have been investigated (1-6). Figure 1 illustrates the preparation of the mannans from the galactomannans by partial acid hydrolysis (6).…”

“…Complement-fixation analysis of the serological reactivities of mannans I with antiserum absorbed with galactomannan II and mannans II with antiserum absorbed with galactomannan I of M. quinckeanum. mannan II, T. interdigitale (4); mannan II, T. rubrum (5); and mannan II, T. schoenleinii (6). Concentration ofpolysaccharides is 200 ,g/ml.…”

Diversity of Antigenic Extracts from the Dermatophyte Trichophyton rubrum./Vergleichende Untersuchung von Antigenextrakten des Dermatophyten Trichophyton rubrum

“…Just like ethylene glycol, extraction with phenol-water (12) and trichloroacetic acid (2) gave glycopeptides. Besides these glycopeptides, polysaccharides (galactomannans and glucan) were found (1,3,4).…”