The xCELLigence system for real-time and label-free analysis of neuronal and dermal cell response to Equine Herpesvirus type 1 infection

Golke, Anna; Cymerys, Joanna; Słońska, Anna; Dzieciątkowski, Tomasz; Chmielewska, Anna; Tucholska, Anna; Banbura, M.

doi:10.2478/v10181-011-0126-4

Cited by 13 publications

(15 citation statements)

References 4 publications

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“…The impedance increase correlates to increasing numbers of migrated cells. 16, 17 P69 displayed a flat line in cell index of migration; in contrast, M12 exhibited a strong migration curve tending to upward in 24 h (Figure 1a). This suggests that P69 has a very low motility capacity while M12 endows with the high motility ability.…”

Section: Resultsmentioning

confidence: 97%

MiRNA-296-3p-ICAM-1 axis promotes metastasis of prostate cancer by possible enhancing survival of natural killer cell-resistant circulating tumour cells

et al. 2013

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Natural killer (NK) cells are important in host to eliminate circulating tumour cells (CTCs) in turn preventing the development of tumour cells into metastasis but the mechanisms are very poorly defined. Here we find that the expression level of miR-296-3p is much lower in the non-metastatic human prostate cancer (PCa) cell line P69 than that in the highly metastatic cell line M12, which is derived from P69. We demonstrate that miR-296-3p directly targets and inhibits the expression of intercellular adhesion molecule 1 (ICAM-1) in the malignant M12. The data from clinical tissue microarrays also show that miR-296-3p is frequently upregulated and ICAM-1 is reversely downregulated in PCa. Interestingly, ectopic expression of miR-296-3p in P69 increases the tolerance to NK cells whereas knockdown of miR-296-3p in M12 reduces the resistance to NK cells, which both phenotypes can be rescued by re-expression or silencing of ICAM-1 in P69 and M12, respectively. These results are also manifested in vivo by the decrease in the incidence of pulmonary tumour metastasis exhibited by knockdown of miR-296-3p in M12 when injected into athymic nude mice via tail vein, and consistently down-expression of ICAM-1 reverses this to increase extravasation of CTCs into lungs. Above results suggest that this newly identified miR-296-3p-ICAM-1 axis has a pivotal role in mediating PCa metastasis by possible enhancing survival of NK cell-resistant CTC. Our findings provide novel potential targets for PCa therapy and prognosis.

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Section: Resultsmentioning

confidence: 97%

MiRNA-296-3p-ICAM-1 axis promotes metastasis of prostate cancer by possible enhancing survival of natural killer cell-resistant circulating tumour cells

et al. 2013

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“…Second, it is a label-free technique, so the potential interference of biomarker such as fluorescent or dyes can be avoided. The preclusion of label allows for assessment of [33] Gloke et al (2012) Herpesvirus type 1 Equine dermal cell line and primary murine neuronal cell line [31] Fender et al (2012) Human adenovirus type 3 A549 cell line [43] CHO: Chinese-hamster overy; HBMEC: Human brain microvascular endothelial cell line; HEK: Human embryonic kidney.…”

Section: Advantages Of Rtcamentioning

confidence: 99%

“…The RTCA assay has been utilized in a number of assays relating to functional assessment of microbial and virus infections including bacterial toxin detection, analyzing bacterial and host cell interaction, virus-induced CPE quantification and neutralizing antibody detection [29][30][31]33,[36][37][38][39][40][41][42][43]. The kinetic responses of cells to the microbial and virus infections allow for quantitative and functional assessment not possible with conventional assays.…”

Section: Applications Of Rtca In Infectious Diseasesmentioning

confidence: 99%

“…The RTCA assay has been applied in a number of cell-based assays. These include cytotoxicity, cell adhesion and spreading, cell proliferation, cell invasion, cell migration, cell apoptosis, RNA interference, functional monitoring of receptormediated signaling, stem cell-derived cardiomyocyte beating, bacterial toxin detection, analyzing bacterial and host cell interaction, virus-induced cytopathic effect (CPE) quantification, neutralizing antibody detection, parasite infective activity assessment, as well as functional detection and monitoring of host immune system [29][30][31][32][33][34][35][36][37][38][39][40][41][42][43].…”

Section: Abstract: Clostridium Difficile • Detection • Quantificationmentioning

confidence: 99%

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Real-time cellular analysis for quantitative detection of functionalClostridium difficiletoxin in stool

Huang

Jin³

et al. 2014

Expert Review of Molecular Diagnostics

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Rapid and accurate diagnosis and monitoring of Clostridium difficile infection (CDI) is critical for patient care and infection control. We will briefly review current laboratory techniques for the diagnosis of CDI and identify aspects needing improvement. We will also introduce a real-time cellular analysis (RTCA) assay developed for the diagnosis and monitoring of CDI using electronic impedance to assess the cell status. The RTCA assay uses impedance measurement to detect minute physiological changes in cells cultured on gold microelectrodes embedded in glass substrates in the bottom of microtiter wells. This assay has been adapted for quantitative detection of C. difficile functional toxin directly from stool specimens. Compared to conventional techniques and molecular assays, the RTCA assay provides a valuable tool for the diagnosis of CDI as well as for the assessment of clinical severity and for monitoring therapeutic efficacies.

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“…CPE is a basis for studies using cell cultures. A novel method for the real-time assay of in vitro cells is real-time cell analysis (RTCA) (4,5,9,10,11,14), also applied to diagnosis of viral diseases (2,3,12,13), and it creates a possibility for improvement in that area. The aim of this experiment was to evaluate the applicability of RTCA in CPE detection in SVDV-infected cell cultures.…”

Section: Introductionmentioning

confidence: 99%

Real-time replication of swine vesicular disease virus (SVDV) in cell culture systems in vitro

Paprocka

Kęsy

2015

Bulletin of the Veterinary Institute in Pulawy

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A swine vesicular disease virus (SVDV) replication assay in IB-RS-2, SK-6, and PK-15 cell cultures was performed using the xCELLigence system. The cell status was monitored by impedance measurement, expressed as cell index (CI). Proliferation of particular cells was examined at the beginning of the study. The cells exhibited the ability to form a monolayer, and the CI values increased with the cell culture growth. After about 23 h and while still in the growth phase, the cells were infected with decimal virus dilutions (10 -1 -10 -6 ) containing from 100 000 to 1 median tissue culture infectious doses (TCID50). SVDV replication in cell cultures induced a change in cell index; together with the occurrence of cytopathic effect (CPE), the CI values declined. A significant correlation between the concentration of the virus used and CPE occurrence was found. The results also enabled determination of cell sensitivity to SVDV infection. The highest sensitivity was exhibited by IB-RS-2, followed by SK-6. To conclude, the xCELLigence System was used effectively and evaluated as being an efficient tool for CPE detection and SVDV replication analysis in cell cultures. Compared to the standard method , it enabled a more precise assessment of viral replication based on the quantitative CI measurement, providing additional current information.

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The xCELLigence system for real-time and label-free analysis of neuronal and dermal cell response to Equine Herpesvirus type 1 infection

Cited by 13 publications

References 4 publications

MiRNA-296-3p-ICAM-1 axis promotes metastasis of prostate cancer by possible enhancing survival of natural killer cell-resistant circulating tumour cells

MiRNA-296-3p-ICAM-1 axis promotes metastasis of prostate cancer by possible enhancing survival of natural killer cell-resistant circulating tumour cells

Real-time cellular analysis for quantitative detection of functionalClostridium difficiletoxin in stool

Real-time replication of swine vesicular disease virus (SVDV) in cell culture systems in vitro

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