The Yersinia pseudotuberculosis YplA phospholipase differs in its activity, regulation and secretion from that of the Yersinia enterocolitica YplA

Meysick, Karen C.; Seidman, Jessica C.; Falconio, Jason

doi:10.1016/j.micpath.2009.04.008

Cited by 3 publications

(3 citation statements)

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“…The associated protein PlaS specializes in modulating or regulating PlaA activity. PlaS inhibits PlaA by interacting directly with PlaA, and it facilitates the protection of the cell interior [5,43,44,47]. Our results were consistent with this.…”

Section: Discussionsupporting

confidence: 91%

“…The PlaA used in this study is originally secreted by the type III secretion system (T3SS), similar to S. marcescens PhlA [40] and Yersinia YplA [41]. These PLA1s are secreted through the flagella-related T3SS, which usually mediates the bacterial flagellar formation in E. coli and Serratia species [40,[42][43][44][45][46]. PlaA, PhlA, and YplA have an N-terminal signal peptide that is about 19-23 residues of hydrophobic amino acids [47] and have been predicted to be secreted by the T3SS [48].…”

Section: Discussionmentioning

confidence: 99%

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High-Level Production of Bacteriotoxic Phospholipase A1 in Bacterial Host Pseudomonas fluorescens via ABC Transporter-Mediated Secretion and Inducible Expression

Park

Eom

et al. 2020

Microorganisms

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Bacterial phospholipase A1 (PLA1) is used in various industrial fields because it can catalyze the hydrolysis, esterification, and transesterification of phospholipids to their functional derivatives. It also has a role in the degumming process of crude plant oils. However, bacterial expression of the foreign PLA1-encoding gene was generally hampered because intracellularly expressed PLA1 is inherently toxic and damages the phospholipid membrane. In this study, we report that secretion-based production of recombinant PlaA, a bacterial PLA1 gene, or co-expression of PlaS, an accessory gene, minimizes this harmful effect. We were able to achieve high-level PlaA production via secretion-based protein production. Here, TliD/TliE/TliF, an ABC transporter complex of Pseudomonas fluorescens SIK-W1, was used to secrete recombinant proteins to the extracellular medium. In order to control the protein expression with induction, a new strain of P. fluorescens, which had the lac operon repressor gene lacI, was constructed and named ZYAI strain. The bacteriotoxic PlaA protein was successfully produced in a bacterial host, with help from ABC transporter-mediated secretion, induction-controlled protein expression, and fermentation. The final protein product is capable of degumming oil efficiently, signifying its application potential.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

High-Level Production of Bacteriotoxic Phospholipase A1 in Bacterial Host Pseudomonas fluorescens via ABC Transporter-Mediated Secretion and Inducible Expression

Park

Eom

et al. 2020

Microorganisms

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“…The action of Pla protein enables the pathogen to spread away from the primary infection site and is important for the development of septicemic plague in mouse models [44]. An accessory protein, yplB plays an important role in regulating the action of yplA protein of the pathogen [45]. The proposed method predicts several interactions between the yplB protein and host membrane proteins.…”

Section: 224mentioning

confidence: 99%

Prediction of protein–protein interactions between human host and a pathogen and its application to three pathogenic bacteria

Oruganty

Srinivasan

2011

International Journal of Biological Macromolecules

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Yersinia enterocolitica

Robins-Browne

2014

Food Microbiology

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Projekterne er gennemført som led i Fødevarestyrelsens kontrol, og DTU Fødevareinstituttet har vaeret ansvarlige for det faglige design samt dataanalyse og vurdering Reviewet har efter aftale med Fødevarestyrelsen primaert fokus på kød og daekker områderne: Salmonella, Campylobacter, VTEC, Yersinia enterocolitica, Hygiejneparametre og Antibiotikaresistens. Da Salmonella forekomsten i slagtekyllinger er taet på nul, er det udeladt af reviewet. Afsnittene har hver deres forfatter er skrevet med hver deres stil, men ud fra en faelles skabelon: 3. CKL review VTEC-verotoksin producerende Escherichia coli 4. Yersinia enterocolitica Projekt 28. Salmonella og Yerinia enterocolitica O3 I udskaeringer i dansk og importeret I fersk kølet svinekød i detail.

show abstract

The Yersinia pseudotuberculosis YplA phospholipase differs in its activity, regulation and secretion from that of the Yersinia enterocolitica YplA

Cited by 3 publications

References 50 publications

High-Level Production of Bacteriotoxic Phospholipase A1 in Bacterial Host Pseudomonas fluorescens via ABC Transporter-Mediated Secretion and Inducible Expression

High-Level Production of Bacteriotoxic Phospholipase A1 in Bacterial Host Pseudomonas fluorescens via ABC Transporter-Mediated Secretion and Inducible Expression

Prediction of protein–protein interactions between human host and a pathogen and its application to three pathogenic bacteria

Yersinia enterocolitica

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