The yields of diacetoxyscirpenol produced byFusarium sambucinum cultures isolated from potato tubers and their toxicity to brine shrimps (Artemia salina)

Abstract

The amount of diacetoxyscirpenol (DAS) produced by 14 isolates ofFusarium sambucinum under laboratory conditions has been examined. The isolates were obtained from potato tubers with dry rot symptoms. Yields of DAS ranged from 20 to 330 mg/kg of wheat grain culture (chemical analysis -TLC). Chemical analysis and biological tests showed very similar results.

Production of volatile sesquiterpenes by Fusarium sambucinum strains with different abilities to synthesize trichothecenes

“…F. sambucinum is known to produce toxic metabolites which can be injurious to human and animal health (10). Among the toxins produced by this fungus, trichothecenes form a major group; diacetoxyscirpenol (DAS) is the major toxin, whereas monoacetoxyscirpenol (MAS), T-2 toxin, and neosolaniol are produced in lesser amounts (4,8,17,19). Trichothecenes are biosynthesized from 3-R mevalonic acid in the isoprenoid pathway.…”

Toxicity of mycotoxins of Fusarium sambucinum for feeding in Galleria mellonella

“…sambucinum to also produce T-2 toxin is well documented (Marasas etal., 1984;Desjardins & P lattner, 1989). This ability, together with the fact that the trichothecene concentrations showing an antifeedant activity may be found in agricultural commodities colonized by F. sambucinum (Perkowski et al, 1989), supports the hypothesis that toxic fungal secondary metabolites are produced as a means of protecting food resources and/or avoiding predation (Janzen, 1977;Wicklow, 1984). As far as we are aware, this is the first time that DAS and NEOS have been tested on G. mellonella and that NEOS has been assayed on any insect.…”