The Yin–Yang of Dendrite Morphology: Unity of Actin and Microtubules

Georges, Penelope C.; Hadzimichalis, Norell M.; Sweet, Eric S.; Firestein, Bonnie L.

doi:10.1007/s12035-008-8046-8

Cited by 68 publications

(54 citation statements)

References 173 publications

(177 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Neurite outgrowth requires coordinated remodeling of the actin and microtubule cytoskeleton (25). BMP signaling induces dendrite formation in primary neurons, and we showed previously that BMP7 modulates the actin cytoskeleton (32).…”

Section: Resultsmentioning

confidence: 79%

See 1 more Smart Citation

Microtubule Stabilization by Bone Morphogenetic Protein Receptor-Mediated Scaffolding of c-Jun N-Terminal Kinase Promotes Dendrite Formation

Podkowa

Zhao

Chow

et al. 2010

Molecular and Cellular Biology

View full text Add to dashboard Cite

Neuronal outgrowth occurs via coordinated remodeling of the cytoskeleton involving both actin and microtubules. Microtubule stabilization drives the extending neurite, yet little is known of the molecular mechanisms whereby extracellular cues regulate microtubule dynamics. Bone morphogenetic proteins (BMPs) play an important role in neuronal differentiation and morphogenesis, and BMP7 in particular induces the formation of dendrites. Here, we show that BMP7 induces stabilization of microtubules in both a MAP2-dependent neuronal cell culture model and in dendrites of primary cortical neurons. BMP7 rapidly activates c-Jun N-terminal kinases (JNKs), known regulators of microtubule dynamics, and we show that JNKs associate with the carboxy terminus of the BMP receptor, BMPRII. Activation and binding of JNKs to BMPRII is required for BMP7-induced microtubule stabilization and for BMP7-mediated dendrite formation in primary cortical neurons. These data indicate that BMPRII acts as a scaffold to localize and coordinate cytoskeletal remodeling and thereby provides an efficient means for extracellular cues, such as BMPs, to control neuronal dendritogenesis.Neurons are highly polarized cells comprised of a single long axon, which transmits signals, and multiple shorter dendrites, which are specialized to receive signals. This polarized property of neurons allows for the establishment of appropriate connections within the central nervous system and ensures unidirectional signal propagation (15, 52). Dendrite formation is critical for normal mammalian brain function, including cognition and memory formation, and dendritic abnormalities closely correlate with mental retardation and underlie the pathology of a number of central nervous system disorders, including Down's, Rett, and Fragile X syndromes and lissencephalies (28,30). Although the importance of dendrite morphology is easily appreciated, the signaling mechanisms by which dendrites develop have only recently begun to be identified (2, 4, 5).Neurite outgrowth occurs as a result of coordinated remodeling of the neuronal cytoskeleton involving actin and microtubules (25). In general, neurite outgrowth occurs as a result of local actin instability in the growth cone and coordinated microtubule stabilization that allows microtubules to protrude their dynamic ends more distally (52). Extracellular growth factors have the ability to regulate the cytoskeleton by activating signaling pathways that change the activity, localization, and stability of cytoskeletal regulators (16). However, the molecular mechanisms by which extracellular factors modulate microtubule stability during dendrite formation remain unclear. c-Jun N-terminal kinase (JNK) is a member of the mitogen-activated protein kinase family with a well-documented role in the regulation of gene transcription, cell death, and survival (9, 50). In the nervous system, JNKs have essential functions in the brain during development, neurite formation, regeneration, and memory formation (27,47). Moreover, accumulating evide...

show abstract

Section: Resultsmentioning

confidence: 79%

“…Neurite outgrowth occurs as a result of coordinated remodeling of the neuronal cytoskeleton involving actin and microtubules (25). In general, neurite outgrowth occurs as a result of local actin instability in the growth cone and coordinated microtubule stabilization that allows microtubules to protrude their dynamic ends more distally (52).…”

mentioning

confidence: 99%

Microtubule Stabilization by Bone Morphogenetic Protein Receptor-Mediated Scaffolding of c-Jun N-Terminal Kinase Promotes Dendrite Formation

Podkowa

Zhao

Chow

et al. 2010

Molecular and Cellular Biology

View full text Add to dashboard Cite

show abstract

“…4, 5) a typical indicator of neural cell viability and adhesion [24,25]. Actin is a structural protein that makes up the cytoskeleton and is important for neurite outgrowth [26]. We stained cellular actin with TRITC-phalloidin and imaged with confocal microscopy.…”

Section: Stimulation Of Neuroblastoma Cellsmentioning

confidence: 99%

Nano-fiber scaffold electrodes based on PEDOT for cell stimulation

Bolin

Svennersten

Wang

et al. 2009

Sensors and Actuators B: Chemical

119

View full text Add to dashboard Cite

Electronically conductive and electrochemically active 3D-scaffolds based on electrospun poly(ethylene terephthalate) (PET) nano-fibers are reported. Vapour phase polymerization was employed to achieve an uniform and conformal coating of poly(3,4-ethylenedioxythiophone) doped with tosylate (PEDOT:tosylate) on the nano-fibers. The PEDOT coatings had a large impact on the wettability, turning the hydrophobic PET fibers super-hydrophilic. SH-SY5Y neuroblastoma cells were grown on the PEDOT coated fibers.The SH-SY5Y cells adhered well and showed healthy morphology. These electrically active scaffolds were used to induce Ca 2+ signalling in SH-SY5Y neuroblastoma cells.PEDOT:tosylate coated nano-fibers represents a class of 3D host environments that combines excellent adhesion and proliferation for neuronal cells with the possibility to regulate their signalling.

show abstract

“…Dieses besteht zum größten Teil aus Actin und Tubulin, erhält die Zellform aufrecht und ist für die Fortbewegung unentbehrlich [20]. Speziell interessant ist das Zusammenspiel der Actin-Filamente mit den Mikrotubuli bei der Ausbildung von Wachstumskegeln und Dendriten neuronaler Zellen [21]. Mit bisherigen lichtmikroskopischen Verfahren war eine Trennung beider Strukturen in den feinen Dendriten kaum möglich.…”

Section: Die Rekonstruktion Des Hochauflösungsbildesunclassified

Strukturierte Beleuchtung in der Hochauflösungsmikroskopie

et al. 2010

View full text Add to dashboard Cite

Wer als Zellbiologe hätte sich nicht schon immer gewünscht, mit seinem Lichtmikroskop feinste Details in der Zelle aufzulösen? Denn die Struktur einer biologischen Einheit sagt viel über ihre Funktionsweise und Dynamik aus. Den Strich durch die Rechnung macht nicht die biologische Probe selbst, sondern ein physikalisches Gesetz, welches Ernst Abbe bereits 1873 formulierte [1]. Es besagt, dass die Auflösung eines Weitfeld Lichtmikroskops durch die Beugung fundamental begrenzt ist. Es gibt aber mittlerweile erfolgreiche Ansätze, das Mikroskop und die mikroskopische Abbildung so zu modifizieren, dass eine Auflösung jenseits des Abbeschen Gesetzes möglich wird. Eines dieser Verfahren ist die strukturierte Beleuchtungsmikroskopie (SIM; engl. „Structured Illumination Microscopy”︁).

show abstract

The Yin–Yang of Dendrite Morphology: Unity of Actin and Microtubules

Cited by 68 publications

References 173 publications

Microtubule Stabilization by Bone Morphogenetic Protein Receptor-Mediated Scaffolding of c-Jun N-Terminal Kinase Promotes Dendrite Formation

Microtubule Stabilization by Bone Morphogenetic Protein Receptor-Mediated Scaffolding of c-Jun N-Terminal Kinase Promotes Dendrite Formation

Nano-fiber scaffold electrodes based on PEDOT for cell stimulation

Strukturierte Beleuchtung in der Hochauflösungsmikroskopie

Contact Info

Product

Resources

About